About: A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3

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About: A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3
Creator	Xia, Xianzhu He, Hongbin Hou, Peili Wang, Hongmei Zhao, Guimin
Source	Elsevier; Medline; PMC
abstract	Abstract Bovine respirovirus 3 also known as Bovine parainfluenza virus type 3 (BPIV3) is one of the most important viral respiratory agents of both young and adult cattle. Rapid diagnosis could contribute greatly in containing epidemics and thus avoid economic losses. However, the lack of robust isothermal visual method poses difficulty. In this study, a novel isothermal assay for detecting BPIV3 was established. The method includes a lateral flow dipstick (LFD) assay combined with reverse transcription recombinase polymerase amplification (RT-RPA). First, the analytical sensitivity and specificity of BPIV3 LFD RT-RPA were tested. The LFD RT-RPA assay has a detection limit of up to 100 copies per reaction in 30 min at 38 °C. Then the performance of LFD RT-RPA was evaluated using 95 clinical samples. Compared to qPCR, the LFD RT-RPA assay showed a clinical sensitivity of 94.74%, a clinical specificity of 96.05% and 0.8734 kappa coefficient. These results have demonstrated the efficiency and effectiveness of the method to be developed into a point of care protocol for the diagnosis of BPIV3.
has issue date	2018-10-31(xsd:dateTime)
bibo:doi	10.1016/j.mcp.2018.08.004
bibo:pmid	30138696
has license	els-covid
sha1sum (hex)	89a41b5b9459997c5db0d3ae3bc8418a3945f535
schema:url	https://doi.org/10.1016/j.mcp.2018.08.004
resource representing a document's title	A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3
has PubMed Central identifier	PMC7126874
has PubMed identifier	30138696
schema:publication	Molecular and Cellular Probes
resource representing a document's body	covid:89a41b5b9459997c5db0d3ae3bc8418a3945f535#body_text
is schema:about of	named entity 'assay' named entity 'assay' named entity 'visual' named entity 'effectiveness' named entity 'robust' named entity 'visual' named entity 'RECOMBINASE' named entity 'BOVINE RESPIROVIRUS 3' named entity 'COMBINED' named entity 'VISUAL' named entity 'ANALYTICAL' named entity 'HAVE' named entity 'DETECTION' named entity 'REVERSE TRANSCRIPTION' named entity 'FLOW' named entity 'SPECIFICITY' named entity 'RPA' named entity 'DEMONSTRATED' named entity 'POINT OF CARE' named entity 'RAPID DIAGNOSIS' named entity 'CLINICAL' named entity 'KAPPA' named entity 'METHOD' named entity 'ROBUST' named entity 'LACK' named entity 'DIPSTICK' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'COEFFICIENT' named entity 'RESULTS' named entity 'VIRAL' named entity 'AVOID' named entity 'NOVEL' named entity 'GREATLY' named entity 'COMPARED' named entity 'CARE PROTOCOL' named entity 'CONTAINING' named entity 'EVALUATED' named entity 'BOVINE RESPIROVIRUS 3' named entity 'SAMPLES' named entity 'AMPLIFICATION' named entity 'POLYMERASE' named entity '100' named entity 'ONE OF' named entity 'CATTLE' named entity 'STUDY' named entity 'LATERAL' named entity 'RAPID' named entity 'DIPSTICK' named entity 'RESPIRATORY' named entity 'REACTION' named entity 'EFFECTIVENESS' named entity '30 MIN' named entity 'YOUNG' named entity 'LFD' named entity 'CONTRIBUTE ' named entity 'PERFORMANCE' named entity 'EFFICIENCY' named entity 'ASSAY' named entity 'POLYMERASE' named entity 'COMBINED' named entity 'CLINICAL SENSITIVITY' named entity 'QPCR' named entity 'USING' named entity 'ADULT' named entity 'DIFFICULTY' named entity 'LATERAL' named entity 'KNOWN' named entity 'ESTABLISHED' named entity 'A POINT' named entity 'TESTED' named entity 'REVERSE TRANSCRIPTION' named entity 'THESE' named entity '8734' named entity 'AMPLIFICATION' named entity 'EPIDEMICS' named entity 'FLOW' named entity 'UP TO'

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