About: Screening efficient siRNAs in vitro as the candidate genes for chicken anti-avian influenza virus H5N1 breeding

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About: Screening efficient siRNAs in vitro as the candidate genes for chicken anti-avian influenza virus H5N1 breeding Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Screening efficient siRNAs in vitro as the candidate genes for chicken anti-avian influenza virus H5N1 breeding
Creator	Wan, J Liu, W Wang, J Zhang, P
Source	PMC
abstract	The frequent disease outbreaks caused by avian influenza virus (AIV) not only affect the poultry industry but also pose a threat to human safety. To address the problem, RNA interference (RNAi) has recently been widely used as a potential antiviral approach. Transgenesis, in combination with RNAi to specifically inhibit AIV gene expression, has been proposed to make chickens resistant to avian influenza. For the transgenic breeding, screening the efficient siRNAs in vitro as the candidate genes is one of the most important tasks. Here, we combined an online search tool and a series of bioinformatics programs with a set of rules for designing the siRNAs targeting different mRNA regions of AIV H5N1 subtype. By this method we chose five rational siRNAs, constructed five U6 promoter-driven shRNA expression plasmids contained the siRNA genes, and used these to produce stably transfected Madin-Darby canine kidney (MDCK) cells. Data from virus titration, IFA, PUI-stained flow cytometry, real-time quantitative RT-PCR and DAS-ELISA analyses showed that all five stably transfected cell lines were effectively resistant to viral replication when exposed to 100 CCID50 of AIV, and we finally chose the most effective plasmids (pSi-604i and pSi-1597i) as the candidates for making the transgenic chickens. These findings provide baseline information for breeding transgenic chickens resistant to AIV in combination with RNAi.
has issue date	2010-03-04(xsd:dateTime)
bibo:doi	10.1134/s0026893310010061
has license	no-cc
sha1sum (hex)	8a9e26bb5e249e78683657b20d65ddec56646aa4
schema:url	https://doi.org/10.1134/s0026893310010061
resource representing a document's title	Screening efficient siRNAs in vitro as the candidate genes for chicken anti-avian influenza virus H5N1 breeding
has PubMed Central identifier	PMC7089267
schema:publication	Mol Biol
resource representing a document's body	covid:8a9e26bb5e249e78683657b20d65ddec56646aa4#body_text
is schema:about of	named entity 'transgenic' named entity 'breeding' named entity 'programs' named entity 'transgenic' named entity 'Genes' named entity 'CANDIDATE GENES' named entity 'PSI' named entity 'PROBLEM' named entity 'TO PRODUCE' named entity 'SHRNA' named entity 'METHOD' named entity 'EFFECTIVE' named entity 'ONE OF' named entity 'AFFECT' named entity 'BASELINE' named entity 'CONTAINED' named entity 'PROMOTER' named entity 'CHOSE' named entity 'ANTIVIRAL' named entity 'PROPOSED' named entity 'QUANTITATIVE' named entity 'EXPOSED TO' named entity 'REGIONS' named entity 'SERIES' named entity 'AIV' named entity 'SIRNA' named entity 'PROGRAMS' named entity 'SAFETY' named entity 'INFORMATION' named entity 'FINDINGS' named entity 'EFFICIENT' named entity 'VIRAL REPLICATION' named entity '100' named entity 'PROVIDE' named entity 'CAUSED BY' named entity 'POTENTIAL' named entity 'THREAT' named entity 'CONSTRUCTED' named entity 'FLOW CYTOMETRY' named entity 'BUT' named entity 'TARGETING' named entity 'POSE' named entity 'POULTRY INDUSTRY' named entity 'GENES' named entity 'AVIAN INFLUENZA VIRUS' named entity 'RNAI' named entity 'MAKE' named entity 'RULES' named entity 'RESISTANT TO' named entity 'RECENTLY' named entity 'GENE EXPRESSION' named entity 'CHICKENS' named entity 'SEARCH TOOL' named entity 'CANINE' named entity 'REAL TIME' named entity 'ADDRESS' named entity 'MRNA' named entity 'HUMAN' named entity 'IFA' named entity 'HERE' named entity 'CCID' named entity 'COMBINED' named entity 'CHICKEN' named entity 'CELL' named entity 'IN VITRO' named entity 'MOLECULAR BIOLOGY' named entity 'SCREENING' named entity 'H5N1' named entity 'BREEDING' named entity 'AVIAN INFLUENZA VIRUS' named entity 'CANDIDATE GENES' named entity 'CELL LINES' named entity 'SCREENING' named entity 'FREQUENT' named entity 'PLASMIDS' named entity 'ONLINE SEARCH' named entity 'THESE' named entity 'RNA INTERFERENCE' named entity 'H5N1 SUBTYPE' named entity 'AVIAN INFLUENZA'

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