About: Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis

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About: Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis
Creator	Gruffydd-Jones, Tim Gunn-Moore, Danielle Harbour, Dave
topic	covid:8ad08ac16e4ce76f327fabe33ba5600825c411a4#this
Source	Elsevier; Medline; PMC
abstract	A reverse transcriptase-polymerase chain reaction (RT-PCR) assay for the detection of the feline coronavirus (FCoV) genome and a co-cultivation method for the isolation of field strains of FCoV are described. Using the RT-PCR assay to assess blood samples from cats with feline infectious peritonitis (FIP) (n=47) and healthy cats from households with endemic FCoV (n=69) it was shown that approximately 80% of the cats were viraemic, irrespective of their health status. It was also shown that, over a 12-month period, a similar percentage of healthy cats remained viraemic, and that the presence of viraemia did not appear to predispose the cats to the development of FIP. The co-cultivation system proved to be a suitable method for the culture of field strains of FCoV from blood samples, so long as the cultures were maintained for at least 4 weeks. Using this system, followed by the RT-PCR, viraemia was detected as frequently as by RT-PCR on RNA extracted directly from peripheral blood mononuclear cells.
has issue date	1998-07-01(xsd:dateTime)
bibo:doi	10.1016/s0378-1135(98)00210-7
bibo:pmid	9791867
has license	no-cc
sha1sum (hex)	8ad08ac16e4ce76f327fabe33ba5600825c411a4
schema:url	https://doi.org/10.1016/s0378-1135%2898%2900210-7
resource representing a document's title	Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis
has PubMed Central identifier	PMC7117229
has PubMed identifier	9791867
schema:publication	Vet Microbiol
resource representing a document's body	covid:8ad08ac16e4ce76f327fabe33ba5600825c411a4#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/8ad08ac16e4ce76f327fabe33ba5600825c411a4
is schema:about of	named entity 'RT-PCR' named entity 'strains' named entity 'assay' named entity 'field' named entity 'cats' named entity 'HEALTHY' named entity 'REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION' named entity 'FELINE' named entity 'ISOLATION' named entity 'CULTIVATION' named entity 'RT-PCR ASSAY' named entity 'SIMILAR' named entity 'FIP' named entity 'EXTRACTED' named entity 'RNA' named entity 'HEALTHY' named entity 'DETECTION' named entity 'SUITABLE' named entity 'FELINE CORONAVIRUS' named entity 'PCR' named entity '80%' named entity 'FOLLOWED BY' named entity 'USING' named entity 'GENOME' named entity 'RT-PCR' named entity 'FELINE INFECTIOUS PERITONITIS' named entity 'APPROXIMATELY' named entity 'APPEAR' named entity 'BLOOD SAMPLES' named entity 'CLINICAL' named entity 'CULTURE' named entity 'REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION' named entity 'DID' named entity 'A 12' named entity 'CATS' named entity 'CORONAVIRUSES' named entity 'FREQUENTLY' named entity 'DIRECTLY' named entity 'FIELD' named entity 'FELINE INFECTIOUS PERITONITIS' named entity 'CATS' named entity 'DETECTION' named entity 'CULTURE' named entity 'PERIPHERAL BLOOD MONONUCLEAR CELLS' named entity 'BLOOD SAMPLES' named entity 'MONTH' named entity 'METHOD' named entity 'ASSAY' named entity 'PERCENTAGE' named entity '4 WEEKS' named entity 'STRAINS' named entity 'HEALTH STATUS' named entity 'PRESENCE OF' named entity 'DESCRIBED' named entity 'LONG' named entity 'VIRAEMIA' named entity 'HOUSEHOLDS' named entity 'THEIR' named entity 'ENDEMIC' named entity 'DEVELOPMENT' named entity 'MAINTAINED' named entity 'PERIOD' named entity 'SYSTEM' named entity 'ASSESS' named entity 'DETECTED' covid:arg/8ad08ac16e4ce76f327fabe33ba5600825c411a4 named entity 'long' named entity 'isolation' named entity 'chain reaction' named entity 'viraemia' named entity 'RT-PCR' named entity 'n69' named entity 'feline' named entity 'FCoV' named entity 'blood samples' named entity 'endemic' named entity 'RT-PCR'

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