About: Molecular Detection of Streptococcus pyogenes by Strand Invasion Based Amplification Assay

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular Detection of Streptococcus pyogenes by Strand Invasion Based Amplification Assay
Creator	Eboigbodin, Kevin Elf, Sonja Auvinen, Pauliina Hirvonen, Sanna Olli, Jenni
Source	PMC
abstract	INTRODUCTION: Streptococcus pyogenes (group A Streptococcus, GAS) is responsible for a variety of highly communicable infections, accounting for 5–15 and 20–30% of sore throat hospital visits in adults and children, respectively. Prompt diagnosis of GAS can improve the quality of patient care and minimize the unnecessary use of antibiotics. OBJECTIVE: Our objective was to develop an alternative nucleic acid amplification method for the diagnosis of GAS. METHOD: We developed and evaluated a strand invasion based amplification (SIBA) assay to rapidly and specifically detect GAS. The performance of the developed GAS SIBA assay was compared with an established GAS polymerase chain reaction (PCR) assay. RESULTS: The GAS SIBA assay detected small amounts (ten copies) of S. pyogenes DNA within 13 min. The rapid detection time was achieved in part by optimization of magnesium concentration and reaction temperature. The sensitivity and specificity of the GAS SIBA assay for detection of S. pyogenes from clinical specimens were both 100%, and clinical specimens were detected within ~ 8 min of starting the reaction. CONCLUSION: Because the GAS SIBA assay is performed at low and constant temperature, it can be used both in centralized laboratories and for point-of-care testing. Furthermore, given its short detection time and strong analytical performance, the GAS SIBA assay could help to improve patient care and minimize unnecessary prescription of antibiotics.
has issue date	2018-07-03(xsd:dateTime)
bibo:doi	10.1007/s40291-018-0346-8
bibo:pmid	29967988
has license	no-cc
sha1sum (hex)	8affb5947f8f36f87d44577882eeb89f120084df
schema:url	https://doi.org/10.1007/s40291-018-0346-8
resource representing a document's title	Molecular Detection of Streptococcus pyogenes by Strand Invasion Based Amplification Assay
has PubMed Central identifier	PMC7099210
has PubMed identifier	29967988
schema:publication	Mol Diagn Ther
resource representing a document's body	covid:8affb5947f8f36f87d44577882eeb89f120084df#body_text
is schema:about of	named entity 'polymerase' named entity 'patient' named entity 'GAS' named entity 'optimization' named entity 'Detection' named entity 'STREPTOCOCCUS PYOGENES' named entity 'ACCOUNTING' named entity 'USED' named entity 'SHORT' named entity 'COPIES' named entity 'QUALITY' named entity 'STREPTOCOCCUS' named entity 'POLYMERASE CHAIN REACTION' named entity 'CHILDREN' named entity 'STRAND INVASION' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'HELP' named entity 'DIAGNOSIS' covid:arg/8affb5947f8f36f87d44577882eeb89f120084df named entity 'STREPTOCOCCUS PYOGENES' named entity 'OUR' named entity 'HOSPITAL VISITS' named entity 'CENTRALIZED' named entity 'PERFORMED' named entity 'OBJECTIVE' named entity 'HIGHLY' named entity 'INTRODUCTION' named entity 'BASED' named entity 'MOLECULAR' named entity 'STRAND INVASION' named entity 'DETECTION' named entity 'ASSAY' named entity 'AMPLIFICATION' named entity 'PROMPT' named entity 'RAPID' named entity 'NUCLEIC ACID AMPLIFICATION METHOD' named entity 'LOW' named entity 'AMPLIFICATION' named entity 'VARIETY' named entity 'BASED' named entity 'POINT-OF-CARE TESTING' named entity 'SMALL' named entity '100%' named entity 'CLINICAL' named entity 'DETECTION' named entity 'RAPIDLY' named entity 'ANTIBIOTICS' named entity 'RESULTS' named entity 'AMOUNTS' named entity 'COMPARED' named entity 'STRONG' named entity 'METHOD' named entity 'SPECIMENS' named entity 'LABORATORIES' named entity 'CONSTANT' named entity 'ANALYTICAL' named entity 'OPTIMIZATION' named entity 'PATIENT CARE' named entity 'PERFORMANCE' named entity 'EVALUATED' named entity 'USE OF' named entity 'PRESCRIPTION OF' named entity 'TEMPERATURE' named entity '30%' named entity 'DNA' named entity 'GIVEN' named entity 'TIME' named entity 'STARTING' named entity 'SIBA' named entity 'DETECT' named entity 'ADULTS' named entity 'RESPONSIBLE' named entity 'SORE THROAT' named entity 'REACTION TEMPERATURE' named entity 'DETECTED' named entity 'ESTABLISHED' named entity 'REACTION'

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