About: Assessment of Virally Vectored Autoimmunity as a Biocontrol Strategy for Cane Toads

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About: Assessment of Virally Vectored Autoimmunity as a Biocontrol Strategy for Cane Toads Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Assessment of Virally Vectored Autoimmunity as a Biocontrol Strategy for Cane Toads
Creator	Shanmuganathan, Thayalini Venables, Daryl Pallister, Jackie Hyatt, Alex Boyle, Donna Halliday, Damien Hardy, Christopher Robinson, Anthony Siddon, Nicole Voysey, Rhonda
Source	PMC
abstract	BACKGROUND: The cane toad, Bufo (Chaunus) marinus, is one of the most notorious vertebrate pests introduced into Australia over the last 200 years and, so far, efforts to identify a naturally occurring B. marinus-specific pathogen for use as a biological control agent have been unsuccessful. We explored an alternative approach that entailed genetically modifying a pathogen with broad host specificity so that it no longer caused disease, but carried a gene to disrupt the cane toad life cycle in a species specific manner. METHODOLOGY/PRINCIPAL FINDINGS: The adult beta globin gene was selected as the model gene for proof of concept of autoimmunity as a biocontrol method for cane toads. A previous report showed injection of bullfrog tadpoles with adult beta globin resulted in an alteration in the form of beta globin expressed in metamorphs as well as reduced survival. In B. marinus we established for the first time that the switch from tadpole to adult globin exists. The effect of injecting B. marinus tadpoles with purified recombinant adult globin protein was then assessed using behavioural (swim speed in tadpoles and jump length in metamorphs), developmental (time to metamorphosis, weight and length at various developmental stages, protein profile of adult globin) and genetic (adult globin mRNA levels) measures. However, we were unable to detect any differences between treated and control animals. Further, globin delivery using Bohle iridovirus, an Australian ranavirus isolate belonging to the Iridovirus family, did not reduce the survival of metamorphs or alter the form of beta globin expressed in metamorphs. CONCLUSIONS/SIGNIFICANCE: While we were able to show for the first time that the switch from tadpole to adult globin does occur in B. marinus, we were not able to induce autoimmunity and disrupt metamorphosis. The short development time of B. marinus tadpoles may preclude this approach.
has issue date	2011-01-25(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0014576
bibo:pmid	21283623
has license	cc0
sha1sum (hex)	8ba5919b2b4c229c9c1685852cf19a1d727ac220
schema:url	https://doi.org/10.1371/journal.pone.0014576
resource representing a document's title	Assessment of Virally Vectored Autoimmunity as a Biocontrol Strategy for Cane Toads
has PubMed Central identifier	PMC3026784
has PubMed identifier	21283623
schema:publication	PLoS One
resource representing a document's body	covid:8ba5919b2b4c229c9c1685852cf19a1d727ac220#body_text
is schema:about of	named entity 'BIOCONTROL' named entity 'DISEASE' named entity 'DISRUPT' named entity 'VERTEBRATE' named entity 'INTRODUCED INTO' named entity 'PESTS' named entity 'CARRIED' named entity 'CANE' named entity 'AUTOIMMUNITY' named entity 'VECTORED' named entity 'ONE OF' named entity 'SPECIES' named entity 'STRATEGY' named entity 'ASSESSMENT' named entity 'TOADS' named entity 'BUFO' named entity 'UNSUCCESSFUL' named entity 'ALTERNATIVE' named entity 'BIOLOGICAL CONTROL' named entity 'MODIFYING' named entity 'SPECIFIC' named entity 'AUSTRALIA' named entity 'FAR' named entity 'LIFE CYCLE' named entity 'USE' named entity 'NATURALLY OCCURRING' named entity 'BROAD' named entity 'CAUSED' named entity 'HOST SPECIFICITY' named entity 'BUT' named entity 'TO IDENTIFY' named entity 'HAVE' named entity 'APPROACH' named entity 'BACKGROUND' named entity '200' named entity 'AGENT' named entity 'CANE TOAD' named entity 'PATHOGEN' named entity 'YEARS' named entity 'A GENE' covid:arg/8ba5919b2b4c229c9c1685852cf19a1d727ac220 named entity 'Bufo' named entity 'manner' named entity 'pathogen' named entity 'alternative' named entity 'Cane Toads' named entity 'vertebrate' named entity 'biological control agent' named entity 'gene' named entity 'Virally' named entity 'protein' named entity 'Australian National Health and Medical Research Council' named entity 'mice' named entity 'metamorphosis' named entity 'cane toad' named entity 'protein' named entity 'metamorphosis' named entity 'morphological' named entity 'Native species' named entity 'amphibian' named entity 'CSIRO' named entity 'tadpoles' named entity 'antigen' named entity 'tadpole' named entity 'protein' named entity 'Cell mediated immunity' named entity 'E. coli' named entity 'Australia' named entity 'virus replication' named entity 'metamorphs' named entity 'virus' named entity 'NaCl'

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