About: A CRISPR-Cas12a-based specific enhancer for more sensitive detection of SARS-CoV-2 infection

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About: A CRISPR-Cas12a-based specific enhancer for more sensitive detection of SARS-CoV-2 infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A CRISPR-Cas12a-based specific enhancer for more sensitive detection of SARS-CoV-2 infection
Creator	Han, Mei Wang, Li Chen, Wei Liu, Yang Wang, Yunshan Song, Fei Yu, Lei Wang, Jin Wei, Dong Wu, Wenjuan Zhang, Xinxin Gu, Dayong Sun, Yangyang Zhang, Renli Zhao, Guoping Peng, Bo Chen, Shuo Zhu, Yongqiang Liu, Yuchen Huang, Ma, Xiaoling Yang, Guoliang Hou, Tieying Zhao, Huailong Weng, Jianping Zhao, Qianqian Zhou, Yunying Cai, Zhiming Gao, Qiuping Guo, Zhongliang Hu, Xuejiao Huang, Weiren Li, Dechang Li, Wujiao Liu, Lanzheng Nie, Guohui Niu, Liman Wang, Lijun Wen, Donghua Zhang, Xiuming Zheng, Huajun Zhu, Dongyi
Source	MedRxiv
abstract	High Ct-values falling in the grey zone are frequently encountered in SARS-CoV-2 detection by real-time reverse transcription PCR (rRT-PCR) and have brought urgent challenges in diagnosis of samples with low viral load. Based on the single-stranded DNA reporter trans-cleavage activity by Cas12a upon target DNA recognition, we create a Specific Enhancer for detection of PCR-amplified Nucleic Acids (SENA) to confirm SARS-CoV-2 detection through specifically targeting its rRT-PCR amplicons. SENA is highly sensitive, with its limit of detection being at least 2 copies/reaction lower than that of the corresponding rRT-PCR, and highly specific, which identifies both false-negative and false-positive cases in clinic applications. SENA provides effective confirmation for nucleic acid amplification-based molecular diagnosis, and may immediately eliminate the uncertainty problems of rRT-PCR in SARS-CoV-2 clinic detection.
has issue date	2020-06-05(xsd:dateTime)
bibo:doi	10.1101/2020.06.02.20119735
has license	medrxiv
sha1sum (hex)	8c52c61c99757cbcd5a9184cbbe4a0bb654a372f
schema:url	https://doi.org/10.1101/2020.06.02.20119735
resource representing a document's title	A CRISPR-Cas12a-based specific enhancer for more sensitive detection of SARS-CoV-2 infection
resource representing a document's body	covid:8c52c61c99757cbcd5a9184cbbe4a0bb654a372f#body_text
is schema:about of	named entity 'create' named entity 'activity' named entity 'problems' named entity 'molecular diagnosis' named entity 'false-positive' named entity 'SENA' named entity 'samples' named entity 'Acids' named entity 'confirmation' named entity 'falling' named entity 'amplified' named entity 'effective' named entity 'eliminate' named entity 'China' named entity 'rRT-PCR' named entity 'amplicons' named entity 'Cas12a' named entity 'false-negative' named entity 'SARS-CoV-2' named entity 'China' named entity 'SARS-CoV-2' named entity 'titration' named entity 'RNA' named entity 'rRT-PCR' named entity 'nucleic acids' named entity 'preprint' named entity 'causal effects' named entity 'RT-qPCR' named entity 'rRT-PCR' named entity 'preprint' named entity 'RT-qPCR' named entity 'preprint' named entity 'Shanghai' named entity 'rRT-PCR' named entity 'preprint' named entity 'pharyngeal' named entity 'limit of detection' named entity 'June 5' named entity 'amplicons' named entity 'COVID' named entity 'first-order reaction' named entity 'false-negative' named entity 'denaturation' named entity 'medRxiv'

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