About: Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens

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About: Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens
Creator	Jackwood, Mark Boynton, Tye Callison, Scott Hilt, Deborah Robison, Robert Sample, Brenda Swayne, David
Source	Elsevier; Medline; PMC
abstract	It is important to rapidly differentiate infectious bronchitis virus (IBV) from disease agents like highly pathogenic avian influenza virus and exotic Newcastle disease virus, which can be extremely similar in the early stages of their pathogenesis. In this study, we report the development and testing of a real-time RT-PCR assay using a Taqman(®)-labeled probe for early and rapid detection of IBV. The assay amplifies a 143-bp product in the 5′-UTR of the IBV genome and has a limit of detection and quantification of 100 template copies per reaction. All 15 strains of IBV tested as well as two Turkey coronavirus strains were amplified, whereas none of the other pathogens examined, tested positive. Evaluation of the assay was completed with 1329 tracheal swab samples. A total of 680 samples collected from IBV antibody negative birds were negative for IBV by the real-time RT-PCR assay. We tested 229 tracheal swabs submitted to two different diagnostic laboratories and found 79.04% of the tracheal swabs positive for IBV by real-time RT-PCR, whereas only 27.51% of the samples were positive by virus isolation, which is the reference standard test. We also collected a total of 120 tracheal swabs at six different time points from birds experimentally infected with different dosages of IBV and found that, independent of the dose given, the viral load in the trachea plateau at 5 days post-inoculation. In addition, an inverse relationship between the dose of virus given and the viral load at 14 days post-inoculation was observed. Finally, we tested 300 total tracheal swab samples, from a flock of commercial broilers spray vaccinated for IBV in the field. The percentage of birds infected with the IBV vaccine at 3, 7, and 14 days post-vaccination was 58%, 65%, and 83%, respectively, indicating that only slightly more than half the birds were initially infected then the vaccine was subsequently transmitted to other birds in the flock. This observation is significant because coronaviruses, which have a high mutation rate, can revert to pathogenicity when bird-to-bird transmission occurs. The real-time RT-PCR test described herein can be used to rapidly distinguish IBV from other respiratory pathogens, which is important for control of this highly infectious virus. The test was extremely sensitive and specific, and can be used to quantitate viral genomic RNA in clinical samples.
has issue date	2006-08-28(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2006.07.018
bibo:pmid	16934878
has license	no-cc
sha1sum (hex)	8d4b026fa5fc8924284bdfb3922b9cb27b1038a5
schema:url	https://doi.org/10.1016/j.jviromet.2006.07.018
resource representing a document's title	Development and evaluation of a real-time Taqman RT-PCR assay for the detection of infectious bronchitis virus from infected chickens
has PubMed Central identifier	PMC7112890
has PubMed identifier	16934878
schema:publication	J Virol Methods
resource representing a document's body	covid:8d4b026fa5fc8924284bdfb3922b9cb27b1038a5#body_text
is schema:about of	named entity 'DETECTION' named entity 'tested' named entity 'IBV' named entity 'RT-PCR' named entity 'test' named entity 'days' named entity 'RT-PCR ASSAY' named entity 'OBSERVATION' named entity 'TRANSMITTED' named entity 'CONTROL' named entity '143' named entity 'VIRAL' named entity 'SIGNIFICANT' named entity 'TESTING' named entity 'UTR' named entity 'LABORATORIES' named entity 'DESCRIBED' named entity 'COMPLETED' named entity 'TAQMAN' named entity 'RAPIDLY' named entity 'VACCINATION' named entity 'CORONAVIRUSES' named entity 'REAL-TIME RT-PCR' named entity 'BIRDS' named entity 'MUTATION RATE' named entity 'EARLY STAGES' named entity 'MORE THAN' named entity 'REVERT' named entity 'GENOME' named entity 'SLIGHTLY' named entity 'HIGH' named entity 'REACTION' named entity 'NEGATIVE' named entity 'VACCINATED' named entity 'TIME' named entity 'SENSITIVE' named entity 'VIRUS' named entity 'LIMIT OF DETECTION' named entity 'TRACHEAL' named entity '300' named entity 'GENOMIC' named entity 'ADDITION' named entity 'EVALUATION' named entity 'PATHOGENICITY' named entity 'POSITIVE' named entity 'RESPIRATORY PATHOGENS' named entity 'SWABS' named entity 'PRODUCT' named entity 'VACCINE' named entity 'COMMERCIAL' named entity 'INDEPENDENT OF' named entity 'CLINICAL' named entity 'INFECTED' named entity 'REAL-TIME' named entity 'DEVELOPMENT' named entity 'OBSERVED' named entity 'REPORT' named entity 'GIVEN' named entity 'TEMPLATE' named entity 'HAVE' named entity 'CHICKENS' named entity 'USING' named entity 'RELATIONSHIP' named entity 'PATHOGENESIS' named entity 'THEIR' named entity 'DEVELOPMENT' named entity 'PLATEAU' named entity 'SPECIFIC' named entity 'HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS' named entity 'DIAGNOSTIC' named entity 'ASSAY' named entity 'RNA' named entity 'DOSE' named entity 'FOUND' named entity 'SIMILAR'

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