About: MT-PCR panel detection of canine parvovirus (CPV-2): Vaccine and wild-type CPV-2 can be difficult to differentiate in canine diagnostic fecal samples

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About: MT-PCR panel detection of canine parvovirus (CPV-2): Vaccine and wild-type CPV-2 can be difficult to differentiate in canine diagnostic fecal samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	MT-PCR panel detection of canine parvovirus (CPV-2): Vaccine and wild-type CPV-2 can be difficult to differentiate in canine diagnostic fecal samples
Creator	Krockenberger, Mark Ly, Anna Muscatello, Gary Rysnik-Steck, Benjamin Silva, Carolina Zhang, Joshua Norris, Jacqueline Higgins, Damien Meggiolaro, Maira Slapeta, Jan
Source	Elsevier; Medline; PMC
abstract	Abstract Canine parvovirus (CPV-2) remains an important cause of devastating enteritis in young dogs. It can be successfully prevented with live attenuated CPV-2 vaccines when given at the appropriate age and in the absence of maternal antibody interference. Rapid diagnosis of parvoviral enteritis in young dogs is essential to ensuring suitable barrier nursing protocols within veterinary hospitals. The current diagnostic trend is to use multiplexed PCR panels to detect an array of pathogens commonly responsible for diarrhea in dogs. The multiplexed PCR assays do not distinguish wild from vaccine CPV-2. They are highly sensitive and detect even a low level of virus shedding, such as those caused by the CPV-2 vaccine. The aim of this study was to identify the CPV-2 subtypes detected in diagnostic specimens and rule out occult shedding of CPV-2 vaccine strains. For a total of 21 samples that tested positive for CPV-2 in a small animal fecal pathogens diagnostic multiplexed tandem PCR (MT-PCR) panel during 2014–2016 we partially characterized the VP2 gene of CPV-2. Vaccine CPV-2 strain, wild type CPV-2a subtypes and vaccine-like CPV-2b subtypes were detected. High copy number was indicative of wild-type CPV-2a presence, but presence of vaccine-like CPV-2b had a variable copy number in fecal samples. A yardstick approach to a copy number or Ct-value to discriminate vaccine strain from a wild type virus of CPV-2 can be, in some cases, potentially misleading. Therefore, discriminating vaccine strain from a wild type subtype of CPV-2 remains ambitious.
has issue date	2017-06-30(xsd:dateTime)
bibo:doi	10.1016/j.mcp.2017.02.007
bibo:pmid	28254505
has license	els-covid
sha1sum (hex)	8dc402c1d9094683f65a385a921fc1c511e4fef1
schema:url	https://doi.org/10.1016/j.mcp.2017.02.007
resource representing a document's title	MT-PCR panel detection of canine parvovirus (CPV-2): Vaccine and wild-type CPV-2 can be difficult to differentiate in canine diagnostic fecal samples
has PubMed Central identifier	PMC7125668
has PubMed identifier	28254505
schema:publication	Molecular and Cellular Probes
resource representing a document's body	covid:8dc402c1d9094683f65a385a921fc1c511e4fef1#body_text
is schema:about of	named entity 'MATERNAL ANTIBODY' named entity 'SAMPLES' named entity 'CURRENT' named entity 'TO DETECT' named entity 'PARTIALLY' named entity 'ENTERITIS' named entity 'BARRIER NURSING' named entity 'DIAGNOSTIC' named entity 'FECAL' named entity 'IMPORTANT' named entity 'CHARACTERIZED' named entity 'TANDEM' named entity 'VETERINARY HOSPITALS' named entity 'LOW LEVEL' named entity 'AGE' named entity 'CPV' named entity 'WILD' named entity 'SMALL ANIMAL' named entity 'TOTAL' named entity 'SHEDDING' named entity 'samples' named entity 'CPV' named entity 'level' named entity 'cases' named entity 'copy number' named entity 'protocols' named entity 'detect' named entity 'CPV' named entity 'wild-type' named entity 'samples' named entity 'parvovirus' named entity 'CANINE PARVOVIRUS' named entity 'CPV' covid:arg/8dc402c1d9094683f65a385a921fc1c511e4fef1 named entity 'VACCINE STRAIN' named entity 'VACCINES' named entity 'OCCULT' named entity 'VALUE' named entity 'USE' named entity 'PCR' named entity 'WILD-TYPE' named entity 'TESTED' named entity 'INTERFERENCE' named entity 'PANEL' named entity 'HIGH' named entity 'ARRAY' named entity 'RAPID DIAGNOSIS' named entity 'GENE' named entity 'APPROACH' named entity 'DOGS' named entity 'GIVEN' named entity 'DETECT' named entity 'LIKE' named entity 'RESPONSIBLE' named entity 'SUBTYPE' named entity 'DISCRIMINATE' named entity 'CAUSE' named entity 'CANINE PARVOVIRUS' named entity 'ESSENTIAL' named entity 'WILD-TYPE' named entity 'SUITABLE' named entity 'VACCINE' named entity 'DIAGNOSTIC' named entity 'DETECTION' named entity 'FECAL' named entity 'DIFFICULT' named entity 'PANEL' named entity 'CANINE' named entity 'DO NOT' named entity 'NURSING PROTOCOLS' named entity 'DIARRHEA' named entity 'VARIABLE'

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