About: Large-scale, in-house production of viral transport media to support SARS-CoV-2 PCR testing in a multi-hospital healthcare network during the COVID-19 pandemic

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Large-scale, in-house production of viral transport media to support SARS-CoV-2 PCR testing in a multi-hospital healthcare network during the COVID-19 pandemic Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Large-scale, in-house production of viral transport media to support SARS-CoV-2 PCR testing in a multi-hospital healthcare network during the COVID-19 pandemic
Creator	Smith, Kenneth Kirby, James Cheng, Annie Chopelas, Amber Dubois-Coyne, Sarah Mezghani, Rodriguez, Shade Talay, Mustafa
Source	MedRxiv; Medline; PMC
abstract	The COVID-19 pandemic has severely disrupted worldwide supplies of viral transport media (VTM) due to widespread demand for SARS-CoV-2 RT-PCR testing. In response to this ongoing shortage, we began production of VTM in-house in support of diagnostic testing in our hospital network. As our diagnostic laboratory was not equipped for reagent production, we took advantage of space and personnel that became available due to closure of the research division of our medical center. We utilized a formulation of VTM described by the CDC that was simple to produce, did not require filtration for sterilization, and used reagents that were available from commercial suppliers. Performance of VTM was evaluated by several quality assurance measures. Based on Ct values of spiking experiments, we found that our VTM supported highly consistent amplification of the SARS-CoV-2 target (coefficient of variation = 2.95%) using the Abbott RealTime SARS-CoV-2 EUA assay on the Abbott m2000 platform. VTM was also found to be compatible with multiple swab types and, based on accelerated stability studies, able to maintain functionality for at least four months at room temperature. We further discuss how we met logistical challenges associated with large-scale VTM production in a crisis setting including use of staged, assembly line for VTM transport tube production.
has issue date	2020-05-01(xsd:dateTime)
bibo:doi	10.1101/2020.04.29.20085514
bibo:pmid	32511593
has license	no-cc
sha1sum (hex)	8e3e98dc83293e790158123148e2d4ae5723aebb
schema:url	https://doi.org/10.1101/2020.04.29.20085514
resource representing a document's title	Large-scale, in-house production of viral transport media to support SARS-CoV-2 PCR testing in a multi-hospital healthcare network during the COVID-19 pandemic
has PubMed Central identifier	PMC7276020
has PubMed identifier	32511593
schema:publication	medRxiv
resource representing a document's body	covid:8e3e98dc83293e790158123148e2d4ae5723aebb#body_text
is schema:about of	named entity 'values' named entity 'hospital network' named entity 'experiments' named entity 'ongoing' named entity 'EUA' named entity 'supported' named entity 'viral' named entity 'TESTING' named entity 'HOSPITAL' named entity 'LABORATORY' named entity 'EUA' named entity 'VALUES' named entity 'MULTIPLE' named entity 'TUBE' named entity 'HIGHLY' named entity 'DEMAND' named entity 'TARGET' named entity 'STABILITY' named entity 'VTM' named entity 'MEASURES' named entity 'ADVANTAGE' named entity 'STUDIES' named entity 'filtration' named entity 'Abbott' named entity 'setting' named entity 'evaluated' named entity 'reagent' named entity 'coefficient' named entity 'room temperature' named entity 'medical center' named entity 'platform' named entity 'SARS-CoV-2' named entity 'reproducibility' named entity 'virus' named entity 'tissue culture' named entity 'medRxiv' named entity 'preprint' named entity 'SARS-CoV-2' named entity 'preprint' named entity 'preprint' named entity 'VTM' named entity 'rock climbing' named entity 'molecular microbiology' named entity 'preprint' named entity 'preprint' named entity 'May 1' named entity 'preprint' named entity 'antimicrobial agents' named entity 'NAAT' named entity 'VTM' named entity 'VTM' named entity 'internal control' named entity 'quality assurance' named entity 'VTM' named entity 'preprint' named entity 'acidic pH' named entity 'nasopharyngeal' named entity 'preprint' named entity 'buffered medium' named entity 'preprint' named entity 'medRxiv' named entity 'VTM' named entity 'Alternative media' named entity 'phenol red' named entity 'VTM' named entity 'production' named entity 'support' named entity 'diagnostic testing' named entity 'consistent' named entity 'multiple' named entity 'SARS-CoV-2' named entity 'testing' named entity 'sterilization' named entity 'diagnostic testing'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software