About: Construction of an infectious bronchitis virus vaccine strain carrying chimeric S1 gene of a virulent isolate and its pathogenicity analysis

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About: Construction of an infectious bronchitis virus vaccine strain carrying chimeric S1 gene of a virulent isolate and its pathogenicity analysis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Construction of an infectious bronchitis virus vaccine strain carrying chimeric S1 gene of a virulent isolate and its pathogenicity analysis
Creator	Zhou, Jiyong Yan, Yan Liao, Min Kumar Ojha, Nishant Shi, Tingting Cao, Shangshang Lv, Chenfei Peng, Xing Zhu, Pengpeng
Source	Medline; PMC
abstract	ABSTRACT: Infectious bronchitis virus (IBV) is a member of genus gamma-coronavirus in the family Coronaviridae, causing serious economic losses to the poultry industry. Reverse genetics is a common technique to study the biological characteristics of viruses. So far, there is no BAC reverse genetic system available for rescue of IBV infectious clone. In the present study, a new strategy for the construction of IBV infectious cDNA clone was established. The full-length genomic cDNA of IBV vaccine strain H120 was constructed in pBAC vector from four IBV fragment subcloning vectors by homologous recombination, which contained the CMV promoter at the 5′ end and the hepatitis D virus ribozyme (HDVR) sequence and bovine growth hormone polyadenylation (BGH) sequence after the polyA tail at the 3′ end of the full-length cDNA. Subsequently, using the same technique, another plasmid pBAC-H120/SCS1 was also constructed, in which S1 gene from IBV H120 strain was replaced with that of a virulent SC021202 strain. Recombinant virus rH120 and rH120/SCS1 were rescued by transfecting the plasmids into BHK cells and passaged in embryonated chicken eggs. Finally, the pathogenicity of both the recombinant virus strains rH120 and rH120/SCS1 was evaluated in SPF chickens. The results showed that the chimeric rH120/SCS1 strain was not pathogenic compared with the wild-type IBV SC021202 strain and the chickens inoculated with rH120/SCS1 could resist challenge infection by IBV SC021202. Taken together, our results indicate that BAC reverse genetic system could be used to rescue IBV in vitro and IBV S1 protein alone might not be the key factor for IBV pathogenicity. KEY POINTS: • BAC vector was used to construct IBV full-length cDNA by homologous recombination. • Based on four subcloning vectors, a recombinant chimeric IBV H120/SCS1 was constructed and rescued. • Pathogenicity of H120/SCS1 was similar to that of H120, but different to that of SC021202.
has issue date	2020-08-19(xsd:dateTime)
bibo:doi	10.1007/s00253-020-10834-2
bibo:pmid	32813067
has license	no-cc
sha1sum (hex)	8e8d6a18fc2e7a1abf016a4821d5b1820ea6a741
schema:url	https://doi.org/10.1007/s00253-020-10834-2
resource representing a document's title	Construction of an infectious bronchitis virus vaccine strain carrying chimeric S1 gene of a virulent isolate and its pathogenicity analysis
has PubMed Central identifier	PMC7434845
has PubMed identifier	32813067
schema:publication	Appl Microbiol Biotechnol
resource representing a document's body	covid:8e8d6a18fc2e7a1abf016a4821d5b1820ea6a741#body_text
is schema:about of	named entity 'wild-type' named entity 'rescued' named entity 'IBV' named entity 'construction' named entity 'IBV' named entity 'SPF' named entity 'IBV' named entity 'embryonated' named entity 'SEQUENCE' named entity 'STUDY' named entity 'FRAGMENT' named entity 'POULTRY INDUSTRY' named entity 'INFECTIOUS' named entity 'VACCINE STRAIN' named entity 'BIOLOGICAL' named entity 'PLASMID' named entity 'CMV PROMOTER' named entity 'PROTEIN ' named entity 'NEW' named entity 'SUBCLONING' named entity 'RESCUE' named entity 'RESULTS' named entity 'POLYADENYLATION' named entity 'OUR' named entity 'STRAIN' named entity 'cDNA' named entity 'vaccine' named entity 'contained' named entity 'rescue' named entity 'CMV promoter' named entity 'genus' named entity 'resist' named entity 'pathogenic' named entity 'fragment' named entity 'poultry industry' named entity 'Infectious bronchitis virus' named entity 'factor' named entity 'strain' named entity '3′ end' named entity 'vector' named entity 'cDNA' named entity 'ribozyme' named entity 'poultry industry' named entity 'IBV' named entity 'gene' named entity 'inoculated' named entity 'IBV' named entity 'genus' named entity 'IBV' named entity 'pathogenicity' named entity 'gene' named entity 'strategy' named entity 'IBV' named entity 'optical density' named entity 'subcloning' named entity 'virus' named entity 'chimeric vaccine' named entity 'restriction enzymes' named entity 'IBV' named entity 'Scobey' named entity 'immunoprotection' named entity 'control group' named entity 'oligonucleotide' named entity 'pathogenicity' named entity 'poultry industry' named entity '5′UTR' named entity 'primers' named entity 'control group' named entity 'oropharyngeal' named entity 'DMEM' named entity 'recombinant protein' named entity 'genome' named entity 'IBV'

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