About: Development of real‐time fluorescent reverse transcription loop‐mediated isothermal amplification assays for rhinovirus detection

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About: Development of real‐time fluorescent reverse transcription loop‐mediated isothermal amplification assays for rhinovirus detection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of real‐time fluorescent reverse transcription loop‐mediated isothermal amplification assays for rhinovirus detection
Creator	Saito, Shinji Takayama, Ikuyo Odagiri, Takato Takahashi, Hitoshi Nakauchi, Mina Kageyama, \| Kunihiro Oba, \| Semba, Shohei Shiho Nagata, \| Kaida, \| Kubo, \|
Source	Medline; PMC
abstract	Human rhinoviruses (RVs) belong to the genus Enterovirus of the family Picornaviridae, and are classified into RV‐A, ‐B, and ‐C species. Two assays were developed to detect RVs by a real‐time fluorescent reverse transcription loop‐mediated isothermal amplification method: one was designed based on the 5′‐untranslated regions (UTRs) of RV‐A and ‐B, and the other was designed based on the 5′‐UTR of RV‐C. The competence of both assays for the diagnosis of RV infection was tested using isolated viruses and compared with real‐time reverse transcription polymerase chain reaction assays on clinical specimens. Neither assay demonstrated cross‐reactivity with other tested enteroviruses, and they detected 19 out of 21 tested RV‐As and seven out of eight tested RV‐Cs. The specificity of the assays was 100% for the detection of RVs and their sensitivity for RV‐A and RV‐C was 86.3% and 77.3%, respectively, on clinical specimens by the combined use of both assays. Considering that both developed assays were highly specific and detected the majority of recently circulating RVs, they are helpful for the diagnosis of RV infection. Consequently, the results generated by these assays will enhance the surveillance of respiratory illness and the study of the roles of RVs associated with clinical features and disease severity.
has issue date	2019-02-22(xsd:dateTime)
bibo:doi	10.1002/jmv.25427
bibo:pmid	30735248
has license	no-cc
sha1sum (hex)	8e9f24ce4a14bc9a0f4300ea62e1557d1bd0e2a5
schema:url	https://doi.org/10.1002/jmv.25427
resource representing a document's title	Development of real‐time fluorescent reverse transcription loop‐mediated isothermal amplification assays for rhinovirus detection
has PubMed Central identifier	PMC7166982
has PubMed identifier	30735248
schema:publication	J Med Virol
resource representing a document's body	covid:8e9f24ce4a14bc9a0f4300ea62e1557d1bd0e2a5#body_text
is schema:about of	named entity 'untranslated regions' named entity 'family' named entity 'UTRs' named entity 'diagnosis' named entity 'majority' named entity 'developed' named entity 'rhinovirus' named entity 'Development' named entity 'SPECIES' named entity 'GENERATED' named entity 'HIGHLY' named entity 'DEMONSTRATED' named entity 'RECENTLY' named entity 'CONSIDERING' named entity 'SENSITIVITY' named entity 'ASSOCIATED WITH' named entity 'METHOD' named entity 'NEITHER' named entity 'DETECTION' named entity 'ISOLATED' named entity 'RHINOVIRUSES' named entity 'assays' named entity 'Picornaviridae' named entity 'specimens' named entity 'enteroviruses' named entity 'cross-reactivity' named entity 'detection' named entity 'tested' named entity 'RVs' named entity 'real-time' named entity 'genus' named entity 'infection' named entity 'assays' named entity 'infection' named entity 'Human' named entity 'fluorescent' named entity 'rhinovirus' named entity '5′-untranslated' named entity 'infection' named entity 'RV-C' named entity 'reverse transcription loop-mediated isothermal amplification' named entity 'RVs' named entity 'cross-reactivity' named entity 'Picornaviridae' named entity 'rhinovirus' named entity 'DNA Polymerase' named entity 'viral genome' named entity 'strand invasion' named entity 'primers' named entity 'RV-C' named entity 'Thermo Fisher' named entity 'cross-reactivity' named entity 'nucleic acid amplification' named entity 'real-time RT-PCR' named entity 'NASBA' named entity 'serially diluted' named entity 'fluorescent' named entity 'High-Fidelity' named entity 'copy number' named entity 'annealing' named entity 'enteroviruses' named entity 'RVs' named entity 'Promega' named entity 'Thermo Fisher Scientific' named entity 'RV-C' named entity 'RNA' named entity 'cross-reactivity' named entity 'real-time RT-PCR' named entity 'Reverse Transcriptase' named entity 'RNA' named entity 'NASBA'

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