About: Calreticulin as a hydrophilic chimeric molecular adjuvant enhances IgG responses to the spike protein of severe acute respiratory syndrome coronavirus

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About: Calreticulin as a hydrophilic chimeric molecular adjuvant enhances IgG responses to the spike protein of severe acute respiratory syndrome coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Calreticulin as a hydrophilic chimeric molecular adjuvant enhances IgG responses to the spike protein of severe acute respiratory syndrome coronavirus
Creator	Gao, Xiao-Ming Hong, Chao Li, Yue Bao, Wanrong Qiu, Xiang
Source	Medline; PMC
abstract	Fragment 450–650 of the spike (S) protein (S450–650) of severe acute respiratory syndrome‐associated coronavirus (SARS‐CoV) contains epitopes capable of being recognized by convalescent sera of SARS patients. Vaccination of mice with recombinant S450–650 (rS450–650) can induce Abs against SARS‐CoV, although the titer is relatively low. In the present study, a fusion protein linking a fragment (residues 39–272) of murine calreticulin (CRT) to S450–650 in a prokaryotic expression system was created. Compared with target antigen alone, the recombinant fusion product (rS450–650‐CRT) has much improved hydrophilicity and immunogenicity. The S450–650‐specific IgG Abs of BALB/c mice subcutaneously immunized with rS450–650‐CRT were in substantially higher titer (approximately fivefold more). Furthermore, the fusion protein, but not rS450–650 alone, was able to elicit S450–650‐specific IgG responses in T cell deficient nude mice. Given that rCRT/39–272 can drive the maturation of bone‐marrow‐derived dendritic cells, directly activate macrophages and B cells, and also elicit helper T cell responses in vivo, we propose that fragment 39–272 of CRT is an effective molecular adjuvant capable of enhancing target Ag‐specific humoral responses in both a T cell‐dependent and independent manner. Fusion protein rS450–650‐CRT is a potential candidate vaccine against SARS‐CoV infection.
has issue date	2012-07-26(xsd:dateTime)
bibo:doi	10.1111/j.1348-0421.2012.00467.x
bibo:pmid	22530918
has license	no-cc
sha1sum (hex)	8f335dc43c1482aaa7f589e53345f2288019c963
schema:url	https://doi.org/10.1111/j.1348-0421.2012.00467.x
resource representing a document's title	Calreticulin as a hydrophilic chimeric molecular adjuvant enhances IgG responses to the spike protein of severe acute respiratory syndrome coronavirus
has PubMed Central identifier	PMC7168421
has PubMed identifier	22530918
schema:publication	Microbiol Immunol
resource representing a document's body	covid:8f335dc43c1482aaa7f589e53345f2288019c963#body_text
is schema:about of	named entity 'linking' named entity 'patients' named entity 'adjuvant' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'IMMUNIZED' named entity 'INDUCE' named entity 'SARS' named entity 'SPECIFIC' named entity 'PROTEIN ' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'ADJUVANT' named entity 'SPIKE PROTEIN' named entity 'antigen' named entity 'fusion protein' named entity 'CRT' named entity 'mice' named entity 'fusion protein' named entity 'acute' named entity 'improved' named entity 'Calreticulin' named entity 'fusion protein' named entity 'antigen' named entity 'fusion protein' named entity 'mice' named entity 'SARS-CoV' named entity 'spike (S) protein' named entity 'epitopes' named entity 'S450' named entity 'calreticulin' named entity 'spike protein' named entity '28 days' named entity 'murine' named entity 'EGFP' named entity 'CSF' named entity 'macrophages' named entity 'CRT' named entity 'IgG' named entity 'DCs' named entity 'mice' named entity 'IgG' named entity 'APCs' named entity 'nitrilotriacetic acid' named entity 'peripheral blood mononuclear cells' named entity 'CRT' named entity 'Birmingham, AL' named entity 'CRT' named entity 'mice' named entity 'influenza viruses' named entity 'CRT' named entity 'optical density' named entity 'amino acids' named entity 'adherent cells' named entity 'mice' named entity 'indirect immunofluorescence' named entity 'CRT' named entity 'antigen' named entity 'binding domain' named entity 'tumor antigen' named entity 'calcium' named entity 'IgG2a' named entity 'recombinant fusion protein' named entity 'St Louis' named entity 'Novagen' named entity 'immunization' named entity 'optical density' named entity 'antigens' named entity 'CRT' named entity 'virus' named entity 'staining' named entity 'tumor antigen' named entity 'mice' named entity 'fusion protein' named entity 'DNA sequencing' named entity '7.9' named entity 'CRT' named entity 'BALB/c' named entity 'S450'

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