About: A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents

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About: A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents
Creator	Smith, Ina Smith, Greg Warrilow, David Bletchly, Cheryl Harrower, Bruce
Source	Elsevier; Medline; PMC
abstract	Medical and veterinary diagnostic and public health laboratories world-wide are increasingly being called upon to introduce molecular diagnostic tests for both endemic and exotic diseases. This demand has accelerated following increasing terrorism fears. Ironically these same concerns have lead to tightening of both import and export controls preventing many laboratories, particularly those outside of the United States, from gaining access to positive control material. This in turn has prevented many laboratories from introducing much needed molecular diagnostic tests. We describe here a generic approach for preparing synthetic DNA or RNA control material for use in either TaqMan or conventional PCR assays. The production of synthetic controls using this approach does not require cloning or special equipment or facilities beyond that found in any laboratory performing molecular diagnostics. The approach significantly reduces the possibility of contamination or erroneously reporting false-positive reactions due to contamination from positive control material. Synthetic controls produced using this approach have been employed in all molecular diagnostic tests performed in our laboratory and are used irrespective of whether we possess the organism or not.
has issue date	2006-05-04(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2006.03.007
bibo:pmid	16677717
has license	no-cc
sha1sum (hex)	8f49e76027cbc70800e90e07cd272d52ac403602
schema:url	https://doi.org/10.1016/j.jviromet.2006.03.007
resource representing a document's title	A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents
has PubMed Central identifier	PMC7112797
has PubMed identifier	16677717
schema:publication	J Virol Methods
resource representing a document's body	covid:8f49e76027cbc70800e90e07cd272d52ac403602#body_text
is schema:about of	named entity 'REAL-TIME PCR ' named entity 'employed' named entity 'veterinary' named entity 'possibility' named entity 'assays' named entity 'molecular diagnostics' named entity 'positive control' named entity 'reactions' named entity 'describe' named entity 'SYNTHETIC' named entity 'SIMPLE' named entity 'BEING' named entity 'HAVE' named entity 'DUE TO' named entity 'FACILITIES' named entity 'POSITIVE CONTROL' named entity 'SYNTHETIC DNA' named entity 'PCR' named entity 'LEAD' named entity 'IMPORT AND EXPORT' named entity 'DOES NOT' named entity 'PERFORMING' named entity 'NEEDED' named entity 'PERFORMED' named entity 'REPORTING' named entity 'PUBLIC HEALTH' named entity 'TERRORISM' named entity 'PRODUCTION' named entity 'TAQMAN' named entity 'DIAGNOSTIC TESTS' named entity 'BEYOND' named entity 'EITHER' named entity 'CLONING' named entity 'ACCESS' named entity 'DESCRIBE' named entity 'MOLECULAR DIAGNOSTICS' named entity 'GAINING' covid:arg/8f49e76027cbc70800e90e07cd272d52ac403602 named entity 'DISEASE' named entity 'CONCERNS' named entity 'RNA' named entity 'FOLLOWING' named entity 'FEARS' named entity 'CONTAMINATION' named entity 'DISEASES' named entity 'TIGHTENING' named entity 'MEDICAL' named entity 'POSSIBILITY OF' named entity 'DEMAND' named entity 'OUTSIDE' named entity 'DIAGNOSTIC' named entity 'CONVENTIONAL' named entity 'ENDEMIC' named entity 'WIDE' named entity 'HERE' named entity 'ORGANISM' named entity 'LABORATORIES' named entity 'CONTROL' named entity 'SPECIAL EQUIPMENT' named entity 'INCREASING' named entity 'EXOTIC' named entity 'METHOD' named entity 'ENDEMIC' named entity 'PREPARING' named entity 'CONVENTIONAL' named entity 'IDENTIFICATION' named entity 'POSSESS' named entity 'UNITED STATES' named entity 'MATERIAL' named entity 'ACCELERATED' named entity 'INTRODUCE' named entity 'GENERIC' named entity 'FOUND' named entity 'MUCH' named entity 'WORLD' named entity 'EXOTIC' named entity 'SYNTHETIC'

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