About: SARS coronavirus 7a protein blocks cell cycle progression at G0/G1 phase via the cyclin D3/pRb pathway

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About: SARS coronavirus 7a protein blocks cell cycle progression at G0/G1 phase via the cyclin D3/pRb pathway Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	SARS coronavirus 7a protein blocks cell cycle progression at G0/G1 phase via the cyclin D3/pRb pathway
Creator	Wu, Jie Chen, Jiapei Cong, Yuwen Shan, Yajun Yuan, Xiaoling Zhao, Zhenhu Yao, Zhenyu Chen, Bo Dong, Bo Wang, Shenqi
source	Elsevier; Medline; PMC
abstract	Abstract The genome of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) contains four structural genes that are homologous to genes found in other coronaviruses, and also contains six subgroup-specific open reading frames (ORFs). Expression of one of these subgroup-specific genes, ORF7a, resulted in apoptosis via a caspase-dependent pathway. Here, we observed that transient expression of ORF7a protein fused with myc or GFP tags at its N or C terminus inhibited cell growth and prevented BrdU incorporation in different cultural cells, suggesting that ORF7a expression may regulate cell cycle progression. Analysis by flow cytometry demonstrated that ORF7a expression was associated with blockage of cell cycle progression at G0/G1 phase in HEK 293 cells after 24 to 60 h post-transfection. Similar results were observed in COS-7 and Vero cells. Mutation analysis of ORF7a revealed that the domain spanning aa 44–82 of 7a protein was essential for its cytoplasmic localization and for induction of the cell cycle arrest. After analyzing the cellular proteins involving in regulation of cell cycle progression, we demonstrated that ORF7a expression was correlated with a significant reduction of cyclin D3 level of mRNA transcription and expression, and phosphorylation of retinoblastoma (Rb) protein at ser795 and ser809/811, not with the expression of cyclin D1, D2, cdk4 and cdk6 in HEK 293 cells. These results suggest that the insufficient expression of cyclin D3 may cause a decreased activity of cyclin D/cdk4/6, resulting in the inhibition of Rb phosphorylation. Accumulation of hypo- or non-phosphorylated pRb thus prevents cell cycle progression at G0/G1 phase.
has issue date	2006-03-01(xsd:dateTime)
bibo:doi	10.1016/j.virol.2005.10.015
bibo:pmid	16303160
has license	els-covid
sha1sum (hex)	8f696d0f29bee5aa7644047bf40bb54d978fc20e
schema:url	https://doi.org/10.1016/j.virol.2005.10.015
resource representing a document's title	SARS coronavirus 7a protein blocks cell cycle progression at G0/G1 phase via the cyclin D3/pRb pathway
has PubMed Central identifier	PMC7111786
has PubMed identifier	16303160
schema:publication	Virology
resource representing a document's body	covid:8f696d0f29bee5aa7644047bf40bb54d978fc20e#body_text
is schema:about of	named entity 'progression' named entity 'cyclin D3' named entity 'cyclin D3' named entity 'cellular proteins' named entity 'spanning' named entity 'protein' named entity 'arrest' named entity 'COS-7' named entity 'SIMILAR' named entity 'DIFFERENT' named entity 'CYTOPLASMIC' named entity 'CELLS' named entity 'INHIBITION' named entity 'REGULATION OF CELL CYCLE PROGRESSION' named entity 'BLOCKAGE' named entity 'POST' named entity 'CELL CYCLE ARREST' named entity 'ASSOCIATED WITH' named entity 'ANALYZING' named entity 'ANALYSIS' named entity 'PHOSPHORYLATION' named entity 'SUGGEST' named entity 'CONTAINS' named entity 'GENOME' named entity 'ACCUMULATION' named entity 'PHASE' named entity 'SARS CORONAVIRUS' named entity 'VERO CELLS' named entity 'BRDU' named entity 'PROTEINS' named entity 'BLOCKS' named entity 'CELL CYCLE PROGRESSION' named entity 'CYCLIN D3' named entity 'SPECIFIC' named entity 'ASSOCIATED' named entity 'HEK 293 CELLS' named entity 'CORONAVIRUS' named entity 'CYCLIN D1' named entity 'SIGNIFICANT' named entity 'PATHWAY' named entity 'PROTEIN ' named entity 'OPEN READING FRAMES' named entity 'INDUCTION' named entity 'ONE OF' named entity 'ITS' named entity 'CAUSE' named entity 'INVOLVING' named entity 'CELL CYCLE PROGRESSION' named entity 'TAGS' named entity 'DOMAIN' named entity 'CASPASE' named entity 'REVEALED' named entity 'TERMINUS' named entity 'MUTATION ANALYSIS' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'PRB' named entity 'GFP' named entity 'HOMOLOGOUS' named entity 'PROTEIN FUSED' named entity 'MRNA TRANSCRIPTION' named entity 'ESSENTIAL' named entity 'PHOSPHORYLATED' named entity 'HYPO' named entity 'FLOW CYTOMETRY' named entity 'RESULTS' named entity 'TRANSFECTION' named entity 'DECREASED ACTIVITY' named entity 'CELLULAR' named entity 'PHASE' named entity 'ORF7A' named entity 'OBSERVED' named entity 'CULTURAL'

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