About: Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye

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About: Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye
Creator	Silva-Frade, Camila Cardoso, Tereza Carolina, Ana Andrade, Alexandre Bregano, Lívia Ferrari, Heitor Rosa, G
Source	Elsevier; Medline; PMC
abstract	Abstract A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid visual detection of turkey coronavirus (TCoV) infection. The reaction is performed in one step in a single tube at 65 °C for 45 min, with hydroxynaphthol blue (HNB) dye added prior to amplification. The detection limit of the RT-LAMP assay was approximately 102 EID50/50 μl TCoV genome, and no cross-reaction with other avian viruses was observed. The assay was evaluated further in tissue suspensions prepared from the ileum and ileum–caecal junctions of infected turkey embryos; 100% of these samples were positive in the RT-LAMP assay. All individual feces samples collected in the field were considered positive by both conventional RT-PCR and RT-LAMP. In conclusion, RT-LAMP with HNB dye was shown to be a sensitive, simple assay for the rapid diagnosis of TCoV infection, either directly from feces or in association with virus isolation methods.
has issue date	2010-12-31(xsd:dateTime)
bibo:doi	10.1016/j.mcp.2010.08.003
bibo:pmid	20732411
has license	els-covid
sha1sum (hex)	90531cf70a42f755a3a3b4dab604f6e39969285b
schema:url	https://doi.org/10.1016/j.mcp.2010.08.003
resource representing a document's title	Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye
has PubMed Central identifier	PMC7125592
has PubMed identifier	20732411
schema:publication	Molecular and Cellular Probes
resource representing a document's body	covid:90531cf70a42f755a3a3b4dab604f6e39969285b#body_text
is schema:about of	named entity 'assay' named entity 'EID' named entity 'FECES' named entity 'DIRECTLY' named entity 'BLUE' named entity 'EVALUATED' named entity 'VISUAL' named entity 'ASSAY' named entity 'CONSIDERED' named entity 'COLLECTED' named entity 'VIRUSES' named entity 'PREPARED' named entity 'REVERSE-TRANSCRIPTION' named entity 'EMBRYOS' named entity 'SAMPLES' named entity 'AVIAN' named entity 'PERFORMED' named entity 'INFECTED' named entity 'GENOME' named entity 'VIRUS ISOLATION' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'TURKEY CORONAVIRUS' named entity 'TISSUES' named entity 'BLUE DYE' named entity 'ASSOCIATION' named entity 'SINGLE' named entity 'APPROXIMATELY' named entity '50%' named entity 'POSITIVE' named entity 'REACTION' named entity 'ONE STEP' named entity 'MIN' named entity 'ADDED' named entity 'LAMP' named entity 'LAMP' named entity 'FECES' named entity 'REVERSE-TRANSCRIPTION' named entity 'TURKEY CORONAVIRUS' named entity 'IN THE FIELD' named entity 'TUBE' named entity 'CONVENTIONAL' named entity '100%' named entity 'TURKEY' named entity 'EITHER' named entity 'OBSERVED' named entity 'THESE' named entity 'DETECTION LIMIT' named entity 'INDIVIDUAL' named entity 'DYE' named entity 'METHODS' named entity 'SIMPLE' named entity 'SENSITIVE' named entity 'RAPID' named entity 'RT-PCR' named entity 'SUSPENSIONS' named entity 'TISSUE' named entity 'DETECTION' named entity 'ILEUM' named entity 'PRIOR TO' named entity 'RAPID DIAGNOSIS' named entity 'CONCLUSION' named entity 'DETECTION' named entity 'INFECTION' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'VISUAL' named entity 'RT-LAMP ASSAY' named entity 'AMPLIFICATION' covid:arg/90531cf70a42f755a3a3b4dab604f6e39969285b named entity 'rapid' named entity 'virus isolation' named entity 'infection' named entity 'TCoV' named entity 'feces' named entity 'RNA' named entity 'reverse-transcription'

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