About: Vaccination with single plasmid DNA encoding IL-12 and antigens of severe fever with thrombocytopenia syndrome virus elicits complete protection in IFNAR knockout mice

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About: Vaccination with single plasmid DNA encoding IL-12 and antigens of severe fever with thrombocytopenia syndrome virus elicits complete protection in IFNAR knockout mice Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Vaccination with single plasmid DNA encoding IL-12 and antigens of severe fever with thrombocytopenia syndrome virus elicits complete protection in IFNAR knockout mice
Creator	Cho, Nam-Hyuk Chung, Junho Kim, Yuri Kim, Yang Choi, Jeon, Yoon Kang, Jun-Gu Kim, Hong-Il Ro, Hyo-Jin Seo, Yong Shin, Jua Jeon, Lee Id, Hwa
Source	PMC
abstract	Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease caused by SFTS virus (SFTSV) infection. Despite a gradual increase of SFTS cases and high mortality in endemic regions, no specific viral therapy nor vaccine is available. Here, we developed a single recombinant plasmid DNA encoding SFTSV genes, Gn and Gc together with NP-NS fusion antigen, as a vaccine candidate. The viral antigens were fused with Fms-like tyrosine kinase-3 ligand (Flt3L) and IL-12 gene was incorporated into the plasmid to enhance cell-mediated immunity. Vaccination with the DNA provides complete protection of IFNAR KO mice upon lethal SFTSV challenge, whereas immunization with a plasmid without IL-12 gene resulted in partial protection. Since we failed to detect antibodies against surface glycoproteins, Gn and Gc, in the immunized mice, antigen-specific cellular immunity, as confirmed by enhanced antigen-specific T cell responses, might play major role in protection. Finally, we evaluated the degree of protective immunity provided by protein immunization of the individual glycoprotein, Gn or Gc. Although both protein antigens induced a significant level of neutralizing activity against SFTSV, Gn vaccination resulted in relatively higher neutralizing activity and better protection than Gc vaccination. However, both antigens failed to provide complete protection. Given that DNA vaccines have failed to induce sufficient immunogenicity in human trials when compared to protein vaccines, optimal combinations of DNA and protein elements, proper selection of target antigens, and incorporation of efficient adjuvant, need to be further investigated for SFTSV vaccine development.
has issue date	2020-03-20(xsd:dateTime)
bibo:doi	10.1371/journal.pntd.0007813
bibo:pmid	32196487
has license	cc-by
sha1sum (hex)	909d950f950ddc4254a53b73ad1c5ada5a41c057
schema:url	https://doi.org/10.1371/journal.pntd.0007813
resource representing a document's title	Vaccination with single plasmid DNA encoding IL-12 and antigens of severe fever with thrombocytopenia syndrome virus elicits complete protection in IFNAR knockout mice
has PubMed Central identifier	PMC7112229
has PubMed identifier	32196487
schema:publication	PLoS Negl Trop Dis
resource representing a document's body	covid:909d950f950ddc4254a53b73ad1c5ada5a41c057#body_text
is schema:about of	named entity 'IL-12' named entity 'neutralizing' named entity 'tick-borne disease' named entity 'Since' named entity 'Given' named entity 'candidate' named entity 'knockout mice' covid:arg/909d950f950ddc4254a53b73ad1c5ada5a41c057 named entity 'provide' named entity 'degree' named entity 'confirmed' named entity 'individual' named entity 'induce' named entity 'mice' named entity 'plasmid' named entity 'fused' named entity 'partial' named entity 'DNA' named entity 'recombinant' named entity 'adjuvant' named entity 'surface' named entity 'immunogenicity' named entity 'induced' named entity 'DNA' named entity 'responses' named entity 'cell-mediated immunity' named entity 'infection' named entity 'enhance' named entity 'activity' named entity 'protein' named entity 'Severe fever with thrombocytopenia syndrome' named entity 'ligand' named entity 'antigens' named entity 'protein' named entity 'SFTS' named entity 'immunization' named entity 'gene' named entity 'Vaccination' named entity 'virus' named entity 'plasmid DNA' named entity 'DNA vaccination' named entity 'hematological' named entity 'DNA vaccination' named entity 'plasmids' named entity 'assay' named entity 'BD Biosciences' named entity 'gene' named entity 'rat' named entity 'mice' named entity 'mice' named entity 'vaccine' named entity 'IL-12' named entity 'N-terminus' named entity 'infection' named entity 'fever' named entity 'vesicular stomatitis virus' named entity 'NP-40' named entity 'Fujifilm' named entity 'vaccination' named entity 'KO mice' named entity 'mice' named entity 'lysis' named entity 'SFTS' named entity 'IFNAR' named entity 'immune sera' named entity 'cDNA' named entity 'IFNAR' named entity 'protein' named entity 'antigens'

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