About: The landscape of SARS-CoV-2 RNA modifications

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	The landscape of SARS-CoV-2 RNA modifications
Creator	Panning, Marcus Kochs, Georg Ruzsics, Zsolt Grüning, Björn Maier, Wolfgang Nekrutenko, Anton Lother, Achim Díaz I Pedrosa, Núria Fuchs, Jonas Gilsbach, Ralf Miladi, Milad Weigang, Sebastian Weiss, Lisa
topic	covid:90f16b9a54141959561f1fce9eff1d70e35fe179#this
source	BioRxiv
abstract	In 2019 the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the first documented cases of severe lung disease COVID-19. Since then, SARS-CoV-2 has been spreading around the globe resulting in a severe pandemic with over 500.000 fatalities and large economical and social disruptions in human societies. Gaining knowledge on how SARS-Cov-2 interacts with its host cells and causes COVID-19 is crucial for the intervention of novel therapeutic strategies. SARS-CoV-2, like other coronaviruses, is a positive-strand RNA virus. The viral RNA is modified by RNA-modifying enzymes provided by the host cell. Direct RNA sequencing (DRS) using nanopores enables unbiased sensing of canonical and modified RNA bases of the viral transcripts. In this work, we used DRS to precisely annotate the open reading frames and the landscape of SARS-CoV-2 RNA modifications. We provide the first DRS data of SARS-CoV-2 in infected human lung epithelial cells. From sequencing three isolates, we derive a robust identification of SARS-CoV-2 modification sites within a physiologically relevant host cell type. A comparison of our data with the DRS data from a previous SARS-CoV-2 isolate, both raised in monkey renal cells, reveals consistent RNA modifications across the viral genome. Conservation of the RNA modification pattern during progression of the current pandemic suggests that this pattern is likely essential for the life cycle of SARS-CoV-2 and represents a possible target for drug interventions.
has issue date	2020-07-18(xsd:dateTime)
bibo:doi	10.1101/2020.07.18.204362
has license	biorxiv
sha1sum (hex)	90f16b9a54141959561f1fce9eff1d70e35fe179
schema:url	https://doi.org/10.1101/2020.07.18.204362
resource representing a document's title	The landscape of SARS-CoV-2 RNA modifications
schema:publication	bioRxiv
resource representing a document's body	covid:90f16b9a54141959561f1fce9eff1d70e35fe179#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/90f16b9a54141959561f1fce9eff1d70e35fe179
is schema:about of	named entity 'reveals' named entity 'therapeutic' named entity 'fatalities' named entity 'positive' named entity 'large' named entity 'transcripts' named entity 'RNA' named entity 'RNA' named entity 'landscape' named entity 'VIRAL' named entity 'SENSING' named entity 'STRATEGIES' named entity 'CORONAVIRUSES' named entity 'INTERVENTION' named entity 'RNA' named entity 'COVID-19' named entity 'RAISED' named entity 'RESULTING IN' named entity 'NOVEL' named entity 'RENAL' named entity 'PROGRESSION' named entity 'HOST CELLS' named entity 'VIRAL GENOME' named entity 'RNA VIRUS' named entity 'STRAND' named entity 'IS A' named entity 'FATALITIES' named entity 'CAUSED' named entity 'PROVIDED' named entity 'THERAPEUTIC' named entity 'MODIFICATION' named entity 'SITES' named entity 'sequencing' named entity 'precisely' named entity 'viral' named entity 'cell' named entity 'open reading frames' named entity 'SARS-CoV-2' named entity 'enables' named entity 'derive' named entity 'genome' named entity 'progression' named entity 'data' named entity 'isolates' named entity 'strand' named entity 'pandemic' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'RNA virus' named entity 'open reading frames' named entity 'RNA' named entity 'severe acute respiratory syndrome coronavirus 2' named entity 'SARS-CoV-2' named entity 'life cycle' named entity 'COVID-19' named entity 'host cell' named entity 'viral genome' named entity 'SARS-Cov-2' named entity 'FASTQ' named entity 'Calu-3' named entity 'sgRNA' named entity 'RNA modification' named entity 'interferon' named entity 'poly-A' named entity 'viral genome' named entity 'significance level' named entity 'p-value' named entity 'COVID-19 pandemic' named entity 'genomes' named entity 'RNA sequencing'

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