About: Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses

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About: Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses
Creator	Bye, Chris Mercier, Sarah Watson, Sarah Wilkinson, John Harman, Andrew Cunningham, Anthony
Source	Medline; PMC
abstract	The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses can significantly interfere with host cell pathways, the components of which traditional housekeeping genes often encode. In this study we have determined the reliability of 10 housekeeping genes in context of four heavily studied viral infections; human immunodeficiency virus type 1, herpes simplex virus type 1, cytomegalovirus and varicella zoster virus infections using a variety of cell types and virus strains. This provides researchers of these viruses with a shortlist of potential housekeeping genes to use as normalisers for QPCR experiments.
has issue date	2007-12-03(xsd:dateTime)
bibo:doi	10.1186/1743-422x-4-130
bibo:pmid	18053162
has license	cc-by
sha1sum (hex)	910f8cb2d8f7ceb37d996bcbf327b4f5b26ed6d5
schema:url	https://doi.org/10.1186/1743-422x-4-130
resource representing a document's title	Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses
has PubMed Central identifier	PMC2216015
has PubMed identifier	18053162
schema:publication	Virol J
resource representing a document's body	covid:910f8cb2d8f7ceb37d996bcbf327b4f5b26ed6d5#body_text
is schema:about of	named entity 'INFECTED' named entity 'VIROLOGY' named entity 'SUITABLE' named entity 'VIRUSES' named entity 'CONTEXT' named entity 'COMPONENTS' named entity 'USING' named entity 'EXPERIMENTS' named entity 'HAVE' named entity 'PCR ANALYSIS' named entity 'PURPOSES' named entity 'HOUSEKEEPING GENES' named entity 'CHOICE' named entity 'INTERFERE' named entity 'HOUSEKEEPING GENES' named entity 'DETERMINED' named entity 'USE' named entity 'QPCR' named entity 'JOURNAL ' named entity 'HUMAN IMMUNODEFICIENCY VIRUS' named entity 'TO MEASURE' named entity 'CELLULAR' named entity 'CELL TYPES' named entity 'HOST CELL' named entity 'VARIETY' named entity 'QUANTITATIVE REAL TIME PCR' named entity 'DETERMINATION' named entity 'HERPES' named entity 'CELLS' named entity 'HUMAN IMMUNODEFICIENCY VIRUS TYPE 1' named entity 'LEVELS' named entity 'VIRUS' named entity 'PROVIDES' named entity 'TRADITIONAL' named entity 'VIRUSES' named entity 'ESSENTIAL' named entity 'HERPES SIMPLEX VIRUS TYPE 1' named entity 'VARICELLA ZOSTER VIRUS' named entity 'APPROPRIATE' named entity 'HOUSEKEEPING GENE' named entity 'RELIABILITY' named entity 'THESE' named entity 'GENE TRANSCRIPTION' named entity 'INFECTIONS' named entity 'REQUIREMENT' named entity 'PATHWAYS' named entity 'VIRAL INFECTIONS' named entity 'VIRAL AND' named entity 'CYTOMEGALOVIRUS' named entity 'POTENTIAL' named entity 'STRAINS' named entity 'ENCODE' named entity 'PARTICULAR' named entity 'NORMALISATION' named entity 'STUDY' named entity 'RESEARCHERS' named entity 'NORMALISATION' named entity 'human immunodeficiency virus' named entity 'context' named entity 'QPCR' named entity 'encode' named entity 'viral infections' named entity 'context' named entity 'cells' named entity 'herpes' named entity 'housekeeping gene' named entity 'viruses' named entity 'QPCR' named entity 'cell types' named entity 'viral infections' named entity 'QPCR' named entity 'housekeeping genes' named entity 'cytomegalovirus' named entity 'herpes simplex virus type 1' named entity 'real time PCR' named entity 'Burkitt's lymphoma'

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