About: Novel, Divergent Simian Hemorrhagic Fever Viruses in a Wild Ugandan Red Colobus Monkey Discovered Using Direct Pyrosequencing

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About: Novel, Divergent Simian Hemorrhagic Fever Viruses in a Wild Ugandan Red Colobus Monkey Discovered Using Direct Pyrosequencing Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Novel, Divergent Simian Hemorrhagic Fever Viruses in a Wild Ugandan Red Colobus Monkey Discovered Using Direct Pyrosequencing
Creator	Lauck, Michael Hyeroba, David Tumukunde, Alex Weny, Geoffrey Friedrich, Thomas O'connor, David Chapman, Colin Goldberg, Tony Lank, Simon
source	PMC
abstract	BACKGROUND: Simian hemorrhagic fever virus (SHFV) has caused lethal outbreaks of hemorrhagic disease in captive primates, but its distribution in wild primates has remained obscure. Here, we describe the discovery and genetic characterization by direct pyrosequencing of two novel, divergent SHFV variants co-infecting a single male red colobus monkey from Kibale National Park, Uganda. METHODOLOGY/PRINCIPAL FINDINGS: The viruses were detected directly from blood plasma using pyrosequencing, without prior virus isolation and with minimal PCR amplification. The two new SHFV variants, SHFV-krc1 and SHFV-krc2 are highly divergent from each other (51.9% nucleotide sequence identity) and from the SHFV type strain LVR 42-0/M6941 (52.0% and 51.8% nucleotide sequence identity, respectively) and demonstrate greater phylogenetic diversity within SHFV than has been documented within any other arterivirus. Both new variants nevertheless have the same 3′ genomic architecture as the type strain, containing three open reading frames not present in the other arteriviruses. CONCLUSIONS/SIGNIFICANCE: These results represent the first documentation of SHFV in a wild primate and confirm the unusual 3′ genetic architecture of SHFV relative to the other arteriviruses. They also demonstrate a degree of evolutionary divergence within SHFV that is roughly equivalent to the degree of divergence between other arterivirus species. The presence of two such highly divergent SHFV variants co-infecting a single individual represents a degree of within-host viral diversity that exceeds what has previously been reported for any arterivirus. These results expand our knowledge of the natural history and diversity of the arteriviruses and underscore the importance of wild primates as reservoirs for novel pathogens.
has issue date	2011-04-22(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0019056
bibo:pmid	21544192
has license	cc-by
sha1sum (hex)	91312e3e01c328ebc4c8007586a84e8cfbdf45e9
schema:url	https://doi.org/10.1371/journal.pone.0019056
resource representing a document's title	Novel, Divergent Simian Hemorrhagic Fever Viruses in a Wild Ugandan Red Colobus Monkey Discovered Using Direct Pyrosequencing
has PubMed Central identifier	PMC3081318
has PubMed identifier	21544192
schema:publication	PLoS One
resource representing a document's body	covid:91312e3e01c328ebc4c8007586a84e8cfbdf45e9#body_text
is schema:about of	named entity 'Viruses' named entity 'SIMIAN HEMORRHAGIC FEVER' named entity 'UGANDAN' named entity 'DESCRIBE' named entity 'NOVEL' named entity 'INFECTING' named entity 'HERE' named entity 'DIRECT' named entity 'USING' named entity 'CAUSED' named entity 'PYROSEQUENCING' named entity 'RED COLOBUS' named entity 'DIRECT' named entity 'HEMORRHAGIC DISEASE' named entity 'GENETIC CHARACTERIZATION' named entity 'SIMIAN HEMORRHAGIC FEVER VIRUS' named entity 'NOVEL' named entity 'PYROSEQUENCING' named entity 'LETHAL' named entity 'OUTBREAKS' named entity 'COLOBUS MONKEY' named entity 'KIBALE NATIONAL PARK' named entity 'DISTRIBUTION' named entity 'WILD' named entity 'UGANDA' named entity 'BACKGROUND' named entity 'VARIANTS' named entity 'RED COLOBUS' named entity 'DISCOVERY' named entity 'SINGLE MALE' named entity 'PRIMATES' named entity 'BUT' named entity 'OBSCURE' named entity 'HEMORRHAGIC FEVER VIRUSES' named entity 'COLOBUS MONKEY' named entity 'WILD' named entity 'ITS' covid:arg/91312e3e01c328ebc4c8007586a84e8cfbdf45e9 named entity 'caused' named entity 'genetic' named entity 'disease' named entity 'divergent' named entity 'Wild' named entity 'Kibale National Park' named entity 'Pyrosequencing' named entity 'Viruses' named entity 'African monkeys' named entity 'homologs' named entity 'cDNA' named entity 'Little Chalfont' named entity 'SHFV' named entity 'SHFV' named entity 'SHFV' named entity 'viruses' named entity 'GenBank' named entity 'Yellow fever' named entity 'blastx' named entity 'western Uganda' named entity 'primates' named entity 'Phylogenetic analyses' named entity 'primate' named entity 'SHFV' named entity 'non-human primates' named entity 'ORFs' named entity 'cell culture' named entity 'red colobus' named entity 'arterivirus' named entity 'hemorrhagic disease' named entity 'genome sequences' named entity 'SHFV' named entity 'SHFV' named entity 'contig' named entity 'SHFV'

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