About: Production of high titre disabled infectious single cycle (DISC) HSV from a microcarrier culture

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About: Production of high titre disabled infectious single cycle (DISC) HSV from a microcarrier culture Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Production of high titre disabled infectious single cycle (DISC) HSV from a microcarrier culture
Creator	Smith, R Zecchini, T
source	PMC
abstract	Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactors. The cells are infected at an MOI of 0.01 pfu cell-1 and the culture harvested 60–72 h later. The infected cells are removed from the microcarriers by the addition of a hypotonic saline and the virus released by low-pressure disruption techniques. Virus titres achieved are compared to the standard roller bottle process. The resulting material is the starting point for the purification of the DISC-HSV virus.
has issue date	1999-01-01(xsd:dateTime)
bibo:doi	10.1023/a:1008005200711
bibo:pmid	19003370
has license	green-oa
sha1sum (hex)	915b5377664a21415be31ffc4f7788c59e1695f5
schema:url	https://doi.org/10.1023/a:1008005200711
resource representing a document's title	Production of high titre disabled infectious single cycle (DISC) HSV from a microcarrier culture
has PubMed Central identifier	PMC3449936
has PubMed identifier	19003370
schema:publication	Cytotechnology
resource representing a document's body	covid:915b5377664a21415be31ffc4f7788c59e1695f5#body_text
is schema:about of	named entity 'saline' named entity 'cells' named entity 'virus' named entity 'culture' named entity 'hypotonic' »more»

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