About: Identification and genetic characterization of a novel Orthobunyavirus species by a straightforward high-throughput sequencing-based approach

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About: Identification and genetic characterization of a novel Orthobunyavirus species by a straightforward high-throughput sequencing-based approach Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Identification and genetic characterization of a novel Orthobunyavirus species by a straightforward high-throughput sequencing-based approach
Creator	Beth-Din, Adi Cohen-Gihon, Inbar Dorozko, Marina Epstein, Eyal Israeli, Ofir Laskar, Orly Melamed, Sharon Paran, Nir Shifman, Ohad Stein, Dana Wolf, Dana Yitzhaki, Shmuel Zvi, Anat Shapira, Shmuel
source	PMC
abstract	Identification and characterization of novel unknown viruses is of great importance. The introduction of high-throughput sequencing (HTS)-based methods has paved the way for genomics-based detection of pathogens without any prior assumptions about the characteristics of the organisms. However, the use of HTS for the characterization of viral pathogens from clinical samples remains limited. Here, we report the identification of a novel Orthobunyavirus species isolated from horse plasma. The identification was based on a straightforward HTS approach. Following enrichment in cell culture, RNA was extracted from the growth medium and rapid library preparation, HTS and primary bioinformatic analyses were performed in less than 12 hours. Taxonomical profiling of the sequencing reads did not reveal sequence similarities to any known virus. Subsequent application of de novo assembly tools to the sequencing reads produced contigs, of which three showed some similarity to the L, M, and S segments of viruses belonging to the Orthobunyavirus genus. Further refinement of these contigs resulted in high-quality, full-length genomic sequences of the three genomic segments (L, M and S) of a novel Orthobunyavirus. Characterization of the genomic sequence, including the prediction of open reading frames and the inspection of consensus genomic termini and phylogenetic analysis, further confirmed that the novel virus is indeed a new species, which we named Ness Ziona virus.
has issue date	2019-03-04(xsd:dateTime)
bibo:doi	10.1038/s41598-019-40036-4
bibo:pmid	30833612
has license	cc-by
sha1sum (hex)	91c7bd831dbc0f6dbbdb2b6f344419a3cb970794
schema:url	https://doi.org/10.1038/s41598-019-40036-4
resource representing a document's title	Identification and genetic characterization of a novel Orthobunyavirus species by a straightforward high-throughput sequencing-based approach
has PubMed Central identifier	PMC6399452
has PubMed identifier	30833612
schema:publication	Sci Rep
resource representing a document's body	covid:91c7bd831dbc0f6dbbdb2b6f344419a3cb970794#body_text
is schema:about of	named entity 'de novo assembly' named entity 'Orthobunyavirus' named entity 'sequences' named entity 'sequence' named entity 'reveal' named entity 'performed' named entity 'Orthobunyavirus' named entity 'BASED' named entity 'IDENTIFICATION' named entity 'SAMPLES' named entity 'GENUS' named entity 'FOLLOWING' named entity 'HIGH' named entity 'QUALITY' named entity 'GENOMIC' named entity 'APPLICATION' named entity 'TOOLS' named entity 'IDENTIFICATION' named entity 'LIMITED' named entity 'ASSEMBLY' named entity 'ISOLATED' named entity 'ORGANISMS' named entity 'BELONGING' named entity 'VIRUS' named entity '12 HOURS' named entity 'ORTHOBUNYAVIRUS' named entity 'SIMILARITY' named entity 'THESE' named entity 'REFINEMENT' named entity 'SPECIES' named entity 'BIOINFORMATIC' named entity 'PERFORMED' named entity 'DE NOVO' named entity 'PRIOR' named entity 'LIBRARY PREPARATION' named entity 'BASED' named entity 'SEQUENCING READS' named entity 'HIGH-THROUGHPUT SEQUENCING' named entity 'CHARACTERIZATION' named entity 'CHARACTERISTICS' named entity 'REVEAL' named entity 'REPORT' named entity 'ORTHOBUNYAVIRUS' named entity 'APPROACH' named entity 'STRAIGHTFORWARD' named entity 'GENETIC CHARACTERIZATION' named entity 'OPEN' named entity 'VIRUSES' named entity 'KNOWN' named entity 'RNA' named entity 'PLASMA' named entity 'DID' named entity 'SEGMENTS' named entity 'RAPID' named entity 'LESS THAN' named entity 'CLINICAL' named entity 'ENRICHMENT' named entity 'CONTIGS' named entity 'USE OF' named entity 'EXTRACTED' named entity 'SUBSEQUENT' named entity 'ABOUT' named entity 'HORSE' named entity 'SEQUENCE' named entity 'VIRAL PATHOGENS' named entity 'PRIMARY' named entity 'LENGTH' named entity 'NOVEL'

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