About: The SARS-CoV-2 Spike protein has a broad tropism for mammalian ACE2 proteins

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: The SARS-CoV-2 Spike protein has a broad tropism for mammalian ACE2 proteins Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	The SARS-CoV-2 Spike protein has a broad tropism for mammalian ACE2 proteins
Creator	Bailey, Dalan Hammond, John Dietrich, Isabelle Shelton, Holly Stevenson-Leggett, Phoebe Graham, Stephen Maier, Helena Bhat, Sushant Bialy, Dagmara Conceicao, Carina Human, Stacey Kelly, James Logan, Leanne Thakur, Nazia Tsirigoti, Christina Varga, Michal Zagrajek, Adrian
source	BioRxiv
abstract	SARS-CoV-2 emerged in late 2019, leading to the COVID-19 pandemic that continues to cause significant global mortality in human populations. Given its sequence similarity to SARS-CoV, as well as related coronaviruses circulating in bats, SARS-CoV-2 is thought to have originated in Chiroptera species in China. However, whether the virus spread directly to humans or through an intermediate host is currently unclear, as is the potential for this virus to infect companion animals, livestock and wildlife that could act as viral reservoirs. Using a combination of surrogate entry assays and live virus we demonstrate that, in addition to human ACE2, the Spike glycoprotein of SARS-CoV-2 has a broad host tropism for mammalian ACE2 receptors, despite divergence in the amino acids at the Spike receptor binding site on these proteins. Of the twenty-two different hosts we investigated, ACE2 proteins from dog, cat and rabbit were the most permissive to SARS-CoV-2, while bat and bird ACE2 proteins were the least efficiently used receptors. The absence of a significant tropism for any of the three genetically distinct bat ACE2 proteins we examined indicates that SARS-CoV-2 receptor usage likely shifted during zoonotic transmission from bats into people, possibly in an intermediate reservoir. Interestingly, while SARS-CoV-2 pseudoparticle entry was inefficient in cells bearing the ACE2 receptor from bats or birds the live virus was still able to enter these cells, albeit with markedly lower efficiency. The apparently broad tropism of SARS-CoV-2 at the point of viral entry confirms the potential risk of infection to a wide range of companion animals, livestock and wildlife.
has issue date	2020-06-18(xsd:dateTime)
bibo:doi	10.1101/2020.06.17.156471
has license	biorxiv
sha1sum (hex)	92afe0816ddc7cf50fbb5d39931ddc5cb010201d
schema:url	https://doi.org/10.1101/2020.06.17.156471
resource representing a document's title	The SARS-CoV-2 Spike protein has a broad tropism for mammalian ACE2 proteins
schema:publication	bioRxiv
resource representing a document's body	covid:92afe0816ddc7cf50fbb5d39931ddc5cb010201d#body_text
is schema:about of	named entity 'bats' named entity '2019' named entity 'continues' named entity 'China' named entity 'human populations' named entity 'ACE2' named entity 'syncytia' named entity 'transfected' named entity 'SARS-CoV-2' named entity 'SARS-CoV' named entity 'closely related' named entity 'fruit bat' named entity 'SARS-CoV-2' named entity 'cell fusion' named entity 'ACE2' named entity 'transfected' named entity 'SARS-CoV-2' named entity 'pangolin' named entity 'SARS-CoV-2' named entity 'biological replicates' named entity 'SARS-CoV' named entity 'ferret' named entity 'coronavirus' named entity 'bat' named entity 'DMEM' named entity 'ACE2' named entity 'ACE2' named entity 'tropism' named entity 'ACE2' named entity 'animal species' named entity 'ACE2' named entity 'glycoprotein' named entity 'PBS' named entity 'brown bats' named entity 'vertebrate species' named entity 'horseshoe bats' named entity 'SARS-CoV-2' named entity 'chicken' named entity 'lentivirus' named entity 'pseudoparticle' named entity 'ACE2' named entity 'infection' named entity 'virus' named entity 'cell culture' named entity 'infection' named entity 'SARS' named entity 'cell fusion' named entity 'DMEM' named entity 'virus particle' named entity 'bat' named entity 'pseudotype' named entity 'bat' named entity 'rat' named entity 'Lentiviral' named entity 'transfection' named entity 'cell fusion' named entity 'SARS-CoV' named entity 'fluorescence' named entity 'virus infections' named entity 'hydrophobic interactions' named entity 'Spike proteins' named entity 'Receptor' named entity 'tropism' named entity '37°C' named entity 'transfection' named entity 'TMPRSS2' named entity 'SARS-CoV-2' named entity 'Spike protein'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software