About: A G-quadruplex-binding macrodomain within the “SARS-unique domain” is essential for the activity of the SARS-coronavirus replication

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About: A G-quadruplex-binding macrodomain within the “SARS-unique domain” is essential for the activity of the SARS-coronavirus replication–transcription complex Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A G-quadruplex-binding macrodomain within the “SARS-unique domain” is essential for the activity of the SARS-coronavirus replication–transcription complex
Creator	Hilgenfeld, Rolf Enjuanes, Luis Kusov, Yuri Tan, Jinzhi Alvarez, Enrique
Source	Elsevier; Medline; PMC
abstract	Abstract The multi-domain non-structural protein 3 of SARS-coronavirus is a component of the viral replication/transcription complex (RTC). Among other domains, it contains three sequentially arranged macrodomains: the X domain and subdomains SUD-N as well as SUD-M within the “SARS-unique domain”. The X domain was proposed to be an ADP-ribose-1”-phosphatase or a poly(ADP-ribose)-binding protein, whereas SUD-NM binds oligo(G)-nucleotides capable of forming G-quadruplexes. Here, we describe the application of a reverse genetic approach to assess the importance of these macrodomains for the activity of the SARS-CoV RTC. To this end, Renilla luciferase-encoding SARS-CoV replicons with selectively deleted macrodomains were constructed and their ability to modulate the RTC activity was examined. While the SUD-N and the X domains were found to be dispensable, the SUD-M domain was crucial for viral genome replication/transcription. Moreover, alanine replacement of charged amino-acid residues of the SUD-M domain, which are likely involved in G-quadruplex-binding, caused abrogation of RTC activity.
has issue date	2015-10-31(xsd:dateTime)
bibo:doi	10.1016/j.virol.2015.06.016
bibo:pmid	26149721
has license	els-covid
sha1sum (hex)	92c57d948da77033283198d299eb4642349a6bee
schema:url	https://doi.org/10.1016/j.virol.2015.06.016
resource representing a document's title	A G-quadruplex-binding macrodomain within the “SARS-unique domain” is essential for the activity of the SARS-coronavirus replication–transcription complex
has PubMed Central identifier	PMC4567502
has PubMed identifier	26149721
schema:publication	Virology
resource representing a document's body	covid:92c57d948da77033283198d299eb4642349a6bee#body_text
is schema:about of	named entity 'BINDING' named entity 'NON-STRUCTURAL PROTEIN 3' named entity 'DELETED' named entity 'COMPLEX' named entity 'THEIR' named entity 'CONTAINS' named entity 'REVERSE' named entity 'RIBOSE' named entity 'CONSTRUCTED' named entity 'CAPABLE' named entity 'G-QUADRUPLEX' named entity 'BINDING' named entity 'GENETIC' named entity 'CAUSED' named entity 'TRANSCRIPTION' named entity 'CHARGED' named entity 'replicons' named entity 'complex' named entity 'arranged' named entity 'involved' named entity 'SARS' named entity 'ESSENTIAL' covid:arg/92c57d948da77033283198d299eb4642349a6bee named entity 'ACTIVITY' named entity 'CORONAVIRUS' named entity 'ACTIVITY' named entity 'ADP' named entity 'UNIQUE' named entity 'COMPLEX' named entity 'LIKELY' named entity 'VIRAL GENOME REPLICATION' named entity 'INVOLVED' named entity 'SUD' named entity 'THESE' named entity 'MULTI' named entity 'ALANINE' named entity 'DOMAIN' named entity 'DESCRIBE' named entity 'FOUND' named entity 'PHOSPHATASE' named entity 'DOMAIN' named entity 'G-QUADRUPLEX' named entity 'TRANSCRIPTION' named entity 'UNIQUE' named entity 'EXAMINED' named entity 'AMINO-ACID RESIDUES' named entity 'BINDING PROTEIN' named entity 'SARS' named entity 'ASSESS' named entity 'NUCLEOTIDES' named entity 'RENILLA LUCIFERASE' named entity 'HERE' named entity 'VIRAL REPLICATION' named entity 'COMPONENT' named entity '28G' named entity 'APPLICATION' named entity 'SARS-COV' named entity 'FORMING' named entity 'IS A' named entity 'OLIGO' named entity 'G-QUADRUPLEXES' named entity 'BINDS' named entity 'APPROACH' named entity 'ENCODING' named entity 'A POLY' named entity 'MODULATE' named entity 'PROPOSED' named entity 'REPLACEMENT' named entity 'END' named entity 'ability' named entity 'domains' named entity 'ADP-ribose' named entity 'RTC' named entity 'The X' named entity 'abrogation' named entity 'alanine' named entity 'domain'

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