About: Quantitative Analysis and Prognostic Implication of SARS Coronavirus RNA in the Plasma and Serum of Patients with Severe Acute Respiratory Syndrome

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About: Quantitative Analysis and Prognostic Implication of SARS Coronavirus RNA in the Plasma and Serum of Patients with Severe Acute Respiratory Syndrome Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Quantitative Analysis and Prognostic Implication of SARS Coronavirus RNA in the Plasma and Serum of Patients with Severe Acute Respiratory Syndrome
Creator	Lee, Nelson Wu, Alan Hui, David Sung, Joseph Chan, K Chim, Stephen Chiu, Rossa Tam, John Dennis Lo, Y Ng, Enders Hung, Emily Tong, Yu
Source	Medline; PMC
abstract	Background: The availability of an early diagnostic tool for severe acute respiratory syndrome (SARS) would have major public health implications. We investigated whether the SARS coronavirus (SARS-CoV) can be detected in serum and plasma samples during the early stages of SARS and studied the potential prognostic implications of such an approach. Methods: We developed two real-time quantitative reverse transcription-PCR (RT-PCR) assays, one for the polymerase and the other for the nucleocapsid region of the virus genome, for measuring the concentration of SARS-CoV RNA in serum/plasma samples from SARS patients. Plasma samples were obtained from 12 confirmed SARS patients on the day of hospital admission, as well as on days 7 and 14 after fever onset. Serum samples were also obtained from 23 confirmed SARS patients on the day of hospital admission, 11 of whom subsequently required intensive care. Viral RNA was extracted from the plasma/serum samples. The extracted RNA was subjected to analysis by the RT-PCR assays. Results: The RT-PCR system for the polymerase region detected SARS-CoV RNA in 50% of plasma and 78% of serum samples from SARS patients during the first week of illness. The detection rates for plasma dropped to 25% at day 14 after fever onset. The median serum SARS-CoV concentrations in patients who required and did not require intensive care unit admission during the course of hospitalization were 5800 and 140 copies/mL, respectively (Mann–Whitney test, P <0.005). These data were confirmed by the RT-PCR system for the nucleocapsid region, which showed an even higher detection rate of 87%. The correlation between the results obtained by the two RT-PCR systems was high (Pearson correlation analysis, r = 0.998; P <0.001). Conclusion: Plasma/serum SARS-CoV quantification represents a potentially useful early diagnostic and prognostic tool for SARS.
has issue date	2003-12-01(xsd:dateTime)
bibo:doi	10.1373/clinchem.2003.024125
bibo:pmid	14633867
has license	no-cc
sha1sum (hex)	93482179ead7f91fc0853dbf1617feccf6207c67
schema:url	https://doi.org/10.1373/clinchem.2003.024125
resource representing a document's title	Quantitative Analysis and Prognostic Implication of SARS Coronavirus RNA in the Plasma and Serum of Patients with Severe Acute Respiratory Syndrome
has PubMed Central identifier	PMC7108161
has PubMed identifier	14633867
schema:publication	Clin Chem
resource representing a document's body	covid:93482179ead7f91fc0853dbf1617feccf6207c67#body_text
is schema:about of	named entity 'genome' named entity 'RNA' named entity 'serum' named entity 'plasma' named entity 'potential' named entity 'high' named entity 'Severe Acute Respiratory Syndrome' named entity 'Plasma' named entity 'PLASMA' named entity 'DIAGNOSTIC TOOL' named entity 'CONCENTRATION OF' named entity 'PCR' named entity 'DATA' named entity 'QUANTIFICATION' named entity 'DETECTED' named entity 'DAY' named entity 'DAYS' named entity 'RNA' named entity 'OBTAINED BY' named entity 'EXTRACTED' named entity 'INTENSIVE CARE' named entity 'QUANTITATIVE REVERSE TRANSCRIPTION-PCR' named entity 'INTENSIVE CARE UNIT' named entity 'CONCLUSION' named entity 'COPIES' named entity 'TEST' named entity 'POTENTIAL' named entity 'DIAGNOSTIC' named entity 'CONFIRMED BY' named entity 'HIGH' named entity 'WEEK' named entity 'NUCLEOCAPSID' named entity 'COURSE' named entity 'EARLY' named entity 'DETECTION' named entity 'CONFIRMED' named entity 'SARS CORONAVIRUS' named entity 'EARLY STAGES' named entity '80%' named entity 'MEDIAN' named entity 'DROPPED' named entity 'SYSTEMS' named entity 'VIRAL RNA' named entity 'HAVE' named entity 'PROGNOSTIC' named entity 'ILLNESS' named entity 'HOSPITALIZATION' named entity 'PATIENTS' named entity 'REGION' named entity 'REAL-TIME' named entity 'SERUM' named entity 'PUBLIC HEALTH' named entity 'SAMPLES' named entity 'RESULTS' named entity 'HOSPITAL ADMISSION' named entity 'FEVER' named entity 'ADMISSION' named entity 'CORRELATION' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'SYSTEM' named entity 'PLASMA' named entity 'ANALYSIS' named entity 'RATES' named entity 'CONCENTRATIONS' named entity 'MAJOR' named entity 'POLYMERASE' named entity 'PROGNOSTIC' named entity 'PATIENTS' named entity 'THESE' named entity '140'

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