About: Molecular characterization of pathogenic 4/91-like and QX-like infectious bronchitis virus infecting commercial poultry farms in Indonesia

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About: Molecular characterization of pathogenic 4/91-like and QX-like infectious bronchitis virus infecting commercial poultry farms in Indonesia Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Molecular characterization of pathogenic 4/91-like and QX-like infectious bronchitis virus infecting commercial poultry farms in Indonesia
Creator	Asmara, Widya Ginting, Teridah Wibowo, Michael
source	Medline; PMC
abstract	BACKGROUND AND AIM: Existing data on the characteristics of infectious bronchitis virus (IBV) gathered throughout Indonesia have been recognized to indicate variants similar to globally distributed vaccine strains. Despite past and current intensive vaccination programs, IBV infections in the country’s poultry industry have not been effectively controlled. Therefore, this study aimed to investigate the genotype of several isolates based on partial S1 gene sequences. In particular, the investigation is directed to focus on layer chickens in actively vaccinated farms indicating IBV symptoms. MATERIALS AND METHODS: Samples were isolated from ten different layer chicken flocks experiencing respiratory problem, drops in egg production, and a “penguin-like” stance, which were collected from commercial poultry farms in Central Java and Yogyakarta regions, Indonesia, within the periods of 2012-2018. Fragment of the S1 gene of IBV sampled from actively vaccinated commercial poultry farms was amplified using primer 5’-aca tgg taa ttt ttc aga tgg-3’ (forward) and 5’-cag att gct tac aac cac c-3’ (reverse) with the length of polymerase chain reaction (PCR) product at 383 bp. The sequence of samples was then compared with the sequence of reference S1 gene nucleotides of IBV from NCBI GenBank database. The amino acid analysis and multiple alignment sequence were conducted using Mega X. RESULTS: During necropsy, enlargement of the oviduct and swollen kidney were observed. Reverse transcription-PCR diagnosis of their 383 bp S1 gene showed that all samples were IBV positive. Phylogenetic analysis of the S1 gene discovered seven samples to be clustered as 4/91-like strains. Meanwhile, the remaining three samples were grouped in QX-like strain cluster. CONCLUSION: This study is a pioneering report providing molecular evidence of pathogenic QX-like and 4/91-like strains circulating in Indonesia. Findings discovered, in this study, strongly suggested the importance of improving protections by available IBV vaccines through updated circulating strain clusters. It is critical to ensure the delivery of an effective control measurement of and vaccination protocols against IBV infections in the country’s commercial poultry industry in particular and worldwide in general.
has issue date	2019-02-20(xsd:dateTime)
bibo:doi	10.14202/vetworld.2019.277-287
bibo:pmid	31040571
has license	cc-by
sha1sum (hex)	936a08054b4018e58ae70402e8918715904451ad
schema:url	https://doi.org/10.14202/vetworld.2019.277-287
resource representing a document's title	Molecular characterization of pathogenic 4/91-like and QX-like infectious bronchitis virus infecting commercial poultry farms in Indonesia
has PubMed Central identifier	PMC6460877
has PubMed identifier	31040571
schema:publication	Vet World
resource representing a document's body	covid:936a08054b4018e58ae70402e8918715904451ad#body_text
is schema:about of	named entity '282' named entity 'FARMS' named entity 'CHARACTERIZATION' named entity 'WORLD' covid:arg/936a08054b4018e58ae70402e8918715904451ad named entity '277' named entity 'POULTRY' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'INDONESIA' named entity 'PATHOGENIC' named entity 'VETERINARY' named entity 'COMMERCIAL' named entity 'INFECTING' named entity 'FARMS' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'POULTRY' named entity 'MOLECULAR' named entity 'LIKE' named entity 'INFECTING' named entity 'HOW' named entity 'ARTICLE' named entity 'COMMERCIAL' named entity 'PATHOGENIC' named entity '287' named entity 'LIKE' named entity 'ASMARA' named entity 'MOLECULAR' named entity 'INDONESIA' named entity 'CHARACTERIZATION' named entity 'CITE' named entity 'World' named entity '2019' named entity 'Indonesia' named entity 'Asmara' named entity 'poultry farms' named entity 'Coronaviridae' named entity 'Central Java' named entity 'IBV' named entity 'vaccination' named entity 'necropsy' named entity 'virus strain' named entity 'allantoic cavity' named entity 'amino acid' named entity 'virus' named entity 'gene' named entity 'histidine' named entity 'nucleoprotein' named entity 'vaccine' named entity 'allantoic' named entity 'gene' named entity 'IBV' named entity 'nephritis' named entity 'Reverse transcriptase' named entity 'poultry' named entity 'vaccine' named entity 'Infectious bronchitis' named entity 'agarose gel' named entity 'distilled water' named entity 'prevention and control' named entity 'PCR' named entity 'IBV' named entity 'poultry' named entity 'tonsil' named entity 'UTR' named entity 'Yogyakarta' named entity 'IBV' named entity 'membrane-bound' named entity 'amino acid' named entity 'poultry' named entity 'virus' named entity 'live attenuated vaccines' named entity 'vaccination' named entity 'IBV' named entity 'respiratory problems' named entity 'infection' named entity 'specific pathogen free' named entity 'vaccination' named entity 'epitopes' named entity 'vaccination'

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