About: High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal

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About: High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal
Creator	Liao, James Baez, Antonino Cho, Kwang-Myung
source	PMC
abstract	Promising approaches to produce higher alcohols, e.g., isobutanol, using Escherichia coli have been developed with successful results. Here, we translated the isobutanol process from shake flasks to a 1-L bioreactor in order to characterize three E. coli strains. With in situ isobutanol removal from the bioreactor using gas stripping, the engineered E. coli strain (JCL260) produced more than 50 g/L in 72 h. In addition, the isobutanol production by the parental strain (JCL16) and the high isobutanol-tolerant mutant (SA481) were compared with JCL260. Interestingly, we found that the isobutanol-tolerant strain in fact produced worse than either JCL16 or JCL260. This result suggests that in situ product removal can properly overcome isobutanol toxicity in E. coli cultures. The isobutanol productivity was approximately twofold and the titer was 9% higher than n-butanol produced by Clostridium in a similar integrated system.
has issue date	2011-03-10(xsd:dateTime)
bibo:doi	10.1007/s00253-011-3173-y
bibo:pmid	21547458
has license	no-cc
sha1sum (hex)	949612898759164b371814f359bc5a6993a1adcc
schema:url	https://doi.org/10.1007/s00253-011-3173-y
resource representing a document's title	High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal
has PubMed Central identifier	PMC3094657
has PubMed identifier	21547458
schema:publication	Appl Microbiol Biotechnol
resource representing a document's body	covid:949612898759164b371814f359bc5a6993a1adcc#body_text
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