About: Structural Definition of a Neutralization-sensitive Epitope on the MERS-CoV S1-NTD

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About: Structural Definition of a Neutralization-sensitive Epitope on the MERS-CoV S1-NTD Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Structural Definition of a Neutralization-sensitive Epitope on the MERS-CoV S1-NTD
Creator	Zhang, Yi Shi, Wei Pallesen, Jesper Wang, Nianshuang Wang, Lingshu Leung, Kwanyee Denison, Mark Becker, Michelle Bowman, Charles Chappell, James Corbett, Kizzmekia Graham, Barney Kirchdoerfer, Robert Mclellan Correspondence, Jason Mclellan, Jason Rosen, Osnat Stevens, Laura Turner, Hannah Ward, Andrew
Source	Elsevier; Medline; PMC
abstract	Middle East respiratory syndrome coronavirus (MERS-CoV) emerged into the human population in 2012 and has caused substantial morbidity and mortality. Potently neutralizing antibodies targeting the receptor-binding domain (RBD) on MERS-CoV spike (S) protein have been characterized, but much less is known about antibodies targeting non-RBD epitopes. Here we report the structural and functional characterization of G2, a neutralizing antibody targeting MERS-CoV S1 N-terminal domain (S1-NTD). Structures of G2 alone and in complex with the MERS-CoV S1-NTD defined a site of vulnerability comprising two loops, each of which contained a residue mutated in G2-escape variants. Cell-surface binding studies and in vitro competition experiments demonstrated that G2 strongly disrupts the attachment of MERS-CoV S to its receptor DDP4, with the inhibition requiring the native trimeric S conformation. These results advance our understanding of antibody-mediated neutralization of coronaviruses and should facilitate development of immunotherapeutics and vaccines against MERS-CoV.
has issue date	2019-09-01(xsd:dateTime)
bibo:doi	10.1016/j.celrep.2019.08.052
bibo:pmid	31553909
has license	gold-oa
sha1sum (hex)	94c76ab70a539f6445ea88bb88dc3197f82ac84c
schema:url	https://doi.org/10.1016/j.celrep.2019.08.052
resource representing a document's title	Structural Definition of a Neutralization-sensitive Epitope on the MERS-CoV S1-NTD
has PubMed Central identifier	PMC6935267
has PubMed identifier	31553909
schema:publication	Cell Reports
resource representing a document's body	covid:94c76ab70a539f6445ea88bb88dc3197f82ac84c#body_text
is schema:about of	named entity 'DPP4' named entity 'binding' named entity 'functional' named entity 'epitope' named entity 'FAB' named entity 'APEX' named entity 'CHARACTERIZATION' named entity 'S PROTEIN' named entity 'PREVENTING' named entity 'DPP4' named entity 'IGG' named entity 'AUTHOR' named entity 'CONSERVED' named entity '28S' named entity 'BINDING' named entity 'EPITOPE' named entity 'NEUTRALIZING ANTIBODY' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'PROTEIN ' named entity 'REPORT' named entity 'BRIEF' named entity 'NTD' named entity 'STRUCTURAL' named entity 'MERS-COV' named entity 'FUNCTIONAL' covid:arg/94c76ab70a539f6445ea88bb88dc3197f82ac84c named entity 'IgG' named entity 'neutralizing antibody' named entity 'neutralizing antibody' named entity 'epitope' named entity 'Middle East respiratory syndrome coronavirus' named entity 'MERS-CoV' named entity 'MERS-CoV' named entity 'IgG' named entity 'MERS-CoV' named entity 'conformation' named entity 'epitopes' named entity 'BHK-21 cells' named entity 'glycoproteins' named entity 'complementarity-determining regions' named entity 'lentiviral' named entity 'MERS-CoV' named entity 'conformation' named entity 'BCoV' named entity 'electron density' named entity 'diffusion' named entity 'pseudotyped' named entity 'MERS-CoV' named entity 'protein' named entity 'MERS-CoV' named entity 'eukaryotic' named entity 'MERS-CoV' named entity 'NTD' named entity 'Strep-tag' named entity 'virus' named entity 'response curve' named entity 'plasmid' named entity 'MHV' named entity 'S protein' named entity 'animal models' named entity 'imidazole' named entity 'DMEM' named entity 'vaccines'

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