About: A Multiple N-Glucosylated Peptide Epitope Efficiently Detecting Antibodies in Multiple Sclerosis

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Multiple N-Glucosylated Peptide Epitope Efficiently Detecting Antibodies in Multiple Sclerosis
Creator	Di Pisa, Margherita Lanzillo, Roberta Lolli, Francesco Morra, Vincenzo Mulinacci, Barbara Nuti, Francesca Paolini, Ilaria Papini, Anna Peroni, Elisa Petruzzo, Martina Real Fernandez, Feliciana Rovero, Paolo Sabatino, Giuseppina Tiberi, Caterina
topic	covid:95c29b58efc1236ba18354ab3b6c04388db6ff0b#this
Source	PMC
abstract	Diagnostics of Multiple Sclerosis (MS) are essentially based on the gold standard magnetic resonance imaging. Few alternative simple assays are available to follow up disease activity. Considering that the disease can remain elusive for years, identification of antibodies fluctuating in biological fluids as relevant biomarkers of immune response is a challenge. In previous studies, we reported that anti-N-glucosylated (N-Glc) peptide antibodies that can be easily detected in Solid-Phase Enzyme-Linked ImmunoSorbent Assays (SP-ELISA) on MS patients’ sera preferentially recognize hyperglucosylated adhesin of non-typeable Haemophilus Influenzae. Since multivalency can be useful for diagnostic purposes to allow an efficient coating in ELISA, we report herein the development of a collection of Multiple N-glucosylated Peptide Epitopes (N-Glc MEPs) to detect anti-N-Glc antibodies in MS. To this aim, a series of N-Glc peptide antigens to be represented in the N-GlcMEPs were tested in competitive ELISA. We confirmed that the epitope recognized by antibodies shall contain at least 5-mer sequences including the fundamental N-Glc moiety. Using a 4-branched dendrimeric lysine scaffold, we selected the N-Glc MEP 24, carrying the minimal epitope Asn(Glc) anchored to a polyethylene glycol-based spacer (PEG) containing a 19-atoms chain, as an efficient multivalent probe to reveal specific and high affinity anti-N-Glc antibodies in MS.
has issue date	2020-07-15(xsd:dateTime)
bibo:doi	10.3390/brainsci10070453
bibo:pmid	32679694
has license	cc-by
sha1sum (hex)	95c29b58efc1236ba18354ab3b6c04388db6ff0b
schema:url	https://doi.org/10.3390/brainsci10070453
resource representing a document's title	A Multiple N-Glucosylated Peptide Epitope Efficiently Detecting Antibodies in Multiple Sclerosis
has PubMed Central identifier	PMC7408607
has PubMed identifier	32679694
schema:publication	Brain Sci
resource representing a document's body	covid:95c29b58efc1236ba18354ab3b6c04388db6ff0b#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/95c29b58efc1236ba18354ab3b6c04388db6ff0b
is schema:about of	named entity 'development' named entity 'Asn' named entity 'immune response' named entity 'EPITOPE' named entity 'assays' named entity 'MEPs' named entity 'high' named entity 'biological fluids' named entity 'Glc' named entity 'antibodies' named entity 'antibodies' named entity 'based' named entity 'Asn' named entity 'Enzyme-Linked ImmunoSorbent Assays' named entity 'glucosylated' named entity 'biomarkers' named entity 'MEP' named entity 'Glc' named entity 'immune response' named entity 'diagnostic purposes' named entity 'Epitopes' named entity 'glucosylated' named entity 'antigens' named entity 'Peptide' named entity 'Antibodies' named entity 'Glucosylated' named entity 'pH 9' named entity 'asparagine' named entity 'Glc' named entity 'moiety' named entity 'antibody' named entity 'Non-parametric' named entity 'IC50' named entity 'MEP' named entity 'FCS' named entity 'glucosylated' named entity 'Lys' named entity 'inflammatory' named entity 'antibodies' named entity 'antibody' named entity 'epitopes' named entity 'glucosylation' named entity 'autoantibody' named entity 'Glc' named entity 'Covid-19' named entity 'antibody' named entity 'IC50' named entity 'Asn' named entity 'multivalent' named entity 'peptide' named entity 'epitope' named entity 'IC50' named entity 'Epitopes' named entity 'lysine' named entity 'statistically significant' named entity 'gold standard' named entity 'multivalent' named entity 'IgM' named entity 'antibodies' named entity 'Peptides' named entity 'epitope' named entity 'IgM antibodies' named entity 'polystyrene' named entity 'multivalent' named entity 'Mann-Whitney' named entity 'inhibitory activity'

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