About: CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication

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About: CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication
Creator	Cong, Xiaoyan Du, Yijun Jiang, Dandan Li, Feng Liu, Sidang Qi, Jing Shan, Hu Shang, Yingli Sui, Chao Wang, Jinqiu Wu, Xiangju
source	Medline; PMC
abstract	Ribonuclease L (RNase L) is an important antiviral endoribonuclease regulated by type I IFN. RNase L is activated by viral infection and dsRNA. Because the role of swine RNase L (sRNase L) is not fully understood, in this study, we generated a sRNase L knockout PK-15 (KO-PK) cell line through the CRISPR/Cas9 gene editing system to evaluate the function of sRNase L. After transfection with CRISPR-Cas9 followed by selection using puromycin, sRNase L knockout in PK-15 cells was further validated by agarose gel electrophoresis, DNA sequencing, and Western blotting. The sRNase L KO-PK cells failed to trigger RNA degradation and induced less apoptosis than the parental PK-15 cells after transfected with poly (I: C). Furthermore, the levels of ISGs mRNA in sRNase L KO-PK cells were higher than those in the parental PK-15 cells after treated with poly (I: C). Finally, both wild type and attenuated pseudorabies viruses (PRV) replicated more efficiently in sRNase L KO-PK cells than the parental PK-15 cells. Taken together, these findings suggest that sRNase L has multiple biological functions including cellular single-stranded RNA degradation, induction of apoptosis, downregulation of transcript levels of ISGs, and antiviral activity against PRV. The sRNase L KO-PK cell line will be a valuable tool for studying functions of sRNase L as well as for producing PRV attenuated vaccine.
has issue date	2019-03-03(xsd:dateTime)
bibo:doi	10.1155/2019/7398208
bibo:pmid	30941371
has license	cc-by
sha1sum (hex)	95d1135211f245b882e54f2842ab328b89066452
schema:url	https://doi.org/10.1155/2019/7398208
resource representing a document's title	CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication
has PubMed Central identifier	PMC6421005
has PubMed identifier	30941371
schema:publication	Biomed Res Int
resource representing a document's body	covid:95d1135211f245b882e54f2842ab328b89066452#body_text
is schema:about of	named entity 'validated' named entity 'RNA' named entity 'cells' named entity 'levels' named entity 'dsRNA' named entity 'Ribonuclease L' named entity 'antiviral' named entity 'cells' named entity 'Cellular' covid:arg/95d1135211f245b882e54f2842ab328b89066452 named entity 'CELLULAR FUNCTION' named entity 'FUNCTIONS' named entity 'GENERATED' named entity 'PARENTAL' named entity 'IMPORTANT' named entity 'STUDY' named entity 'SWINE' named entity 'PRV' named entity 'CELL LINE' named entity 'EVALUATE' named entity 'APOPTOSIS' named entity 'REGULATED' named entity 'TYPE I' named entity 'WILD TYPE' named entity 'DNA SEQUENCING' named entity 'AGAROSE GEL ELECTROPHORESIS' named entity 'LEVELS' named entity 'DSRNA' named entity 'CELLULAR' named entity 'DOWNREGULATION' named entity 'SINGLE-STRANDED RNA' named entity '28I' named entity 'VIRUSES' named entity 'THESE' named entity 'ENDORIBONUCLEASE' named entity 'IFN' named entity 'ROLE' named entity 'TRANSCRIPT' named entity 'FAILED' named entity 'INCLUDING' named entity 'TOOL' named entity 'ACTIVATED' named entity 'WESTERN BLOTTING' named entity 'RNASE L' named entity 'HIGHER' named entity 'REPLICATION' named entity 'FINDINGS' named entity 'SUGGEST' named entity 'USING' named entity 'ANTIVIRAL' named entity 'ANTIVIRAL ACTIVITY' named entity 'SELECTION' named entity 'FULLY' named entity 'REPLICATED' named entity 'MEDIATED' named entity 'PRV' named entity 'BIOLOGICAL FUNCTIONS' named entity 'FOLLOWED BY' named entity 'KNOCKOUT' named entity 'CRISPR-CAS9' named entity 'RNASE L' named entity 'CELLS' named entity 'PRODUCING' named entity 'VIRAL INFECTION' named entity 'INDUCTION OF APOPTOSIS' named entity 'RNA DEGRADATION' named entity 'GENE EDITING' named entity 'TREATED WITH' named entity 'RIBONUCLEASE L' named entity 'MRNA' named entity 'PUROMYCIN'

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