About: Design and development of a chimeric vaccine candidate against zoonotic hepatitis E and foot-and-mouth disease

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About: Design and development of a chimeric vaccine candidate against zoonotic hepatitis E and foot-and-mouth disease Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Design and development of a chimeric vaccine candidate against zoonotic hepatitis E and foot-and-mouth disease
Creator	Liu, Zhenzhen Baha, Sarra Behloul, Nouredine Meng, Jihong Shi, Ruihua Rao, Yuliang Wei, Wenjuan Zhu, Yuanyuan
source	PMC
abstract	BACKGROUND: Zoonotic hepatitis E virus (HEV) infection emerged as a serious threat in the industrialized countries. The aim of this study is exploring a new approach for the control of zoonotic HEV in its main host (swine) through the design and development of an economically interesting chimeric vaccine against HEV and against a devastating swine infection: the foot-and-mouth disease virus (FMDV) infection. RESULTS: First, we adopted a computational approach for rational and effective screening of the different HEV-FMDV chimeric proteins. Next, we further expressed and purified the selected chimeric immunogens in Escherichia coli (E. coli) using molecular cloning techniques. Finally, we assessed the antigenicity and immunogenicity profiles of the chimeric vaccine candidates. Following this methodology, we designed and successfully produced an HEV-FMDV chimeric vaccine candidate (Seq 8-P222) that was highly over-expressed in E. coli as a soluble protein and could self-assemble into virus-like particles. Moreover, the vaccine candidate was thermo-stable and exhibited optimal antigenicity and immunogenicity properties. CONCLUSION: This study provides new insights into the vaccine development technology by using bioinformatics for the selection of the best candidates from larger sets prior to experimentation. It also presents the first HEV-FMDV chimeric protein produced in E. coli as a promising chimeric vaccine candidate that could participate in reducing the transmission of zoonotic HEV to humans while preventing the highly contagious foot-and-mouth disease in swine.
has issue date	2020-07-11(xsd:dateTime)
bibo:doi	10.1186/s12934-020-01394-1
has license	cc-by
sha1sum (hex)	95d4bd5a616c426a957d946c64fa2c35889e292b
schema:url	https://doi.org/10.1186/s12934-020-01394-1
resource representing a document's title	Design and development of a chimeric vaccine candidate against zoonotic hepatitis E and foot-and-mouth disease
has PubMed Central identifier	PMC7352093
schema:publication	Microb Cell Fact
resource representing a document's body	covid:95d4bd5a616c426a957d946c64fa2c35889e292b#body_text
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