About: Original Article: Real time reverse transcription (RRT)‐polymerase chain reaction (PCR) methods for detection of pandemic (H1N1) 2009 influenza virus and European swine influenza A virus infections in pigs

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About: Original Article: Real time reverse transcription (RRT)‐polymerase chain reaction (PCR) methods for detection of pandemic (H1N1) 2009 influenza virus and European swine influenza A virus infections in pigs Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Original Article: Real time reverse transcription (RRT)‐polymerase chain reaction (PCR) methods for detection of pandemic (H1N1) 2009 influenza virus and European swine influenza A virus infections in pigs
Creator	Essen, Steve Gardner, Rebecca Hanna, Amanda Ridgeon, Jonathan Spackman, Erica Brookes, Sharon Brown, Ian Coward, Vivien Densham, Anstice Irvine, Richard Slomka, Marek Suarez, David Marek, Dr Slomka, J Workgroup, Avian
source	Medline; PMC
abstract	Please cite this paper as: Slomka et al. (2010) Real time reverse transcription (RRT)‐polymerase chain reaction (PCR) methods for detection of pandemic (H1N1) 2009 influenza virus and European swine influenza A virus infections in pigs. Influenza and Other Respiratory Viruses 4(5), 277–293. Background There is a requirement to detect and differentiate pandemic (H1N1) 2009 (H1N1v) and established swine influenza A viruses (SIVs) by real time reverse transcription (RRT) PCR methods. Objectives First, modify an existing matrix (M) gene RRT PCR for sensitive generic detection of H1N1v and other European SIVs. Second, design an H1 RRT PCR to specifically detect H1N1v infections. Methods RRT PCR assays were used to test laboratory isolates of SIV (n = 51; 37 European and 14 North American), H1N1v (n = 5) and avian influenza virus (AIV; n = 43). Diagnostic sensitivity and specificity were calculated for swabs (n = 133) and tissues (n = 116) collected from field cases and pigs infected experimentally with SIVs and H1N1v. Results The “perfect match” M gene RRT PCR was the most sensitive variant of this test for detection of established European SIVs and H1N1v. H1 RRT PCR specifically detected H1N1v but not European SIVs. Validation with clinical specimens included comparison with virus isolation (VI) as a “gold standard”, while field infection with H1N1v in swine was independently confirmed by sequencing H1N1v amplified by conventional RT PCR. “Perfect match” M gene RRT PCR had 100% sensitivity and 95·2% specificity for swabs, 93·6% and 98·6% for tissues. H1 RRT PCR demonstrated sensitivity and specificity of 100% and 99·1%, respectively, for the swabs, and 100% and 100% for the tissues. Conclusions Two RRT PCRs for the purposes of (i) generic detection of SIV and H1N1v infection in European pigs, and for (ii) specific detection of H1N1v (pandemic influenza) infection were validated.
has issue date	2010-08-17(xsd:dateTime)
bibo:doi	10.1111/j.1750-2659.2010.00149.x
bibo:pmid	20716157
has license	no-cc
sha1sum (hex)	96cd30cbd4f3cecd2d0add83df270e1ae68670b1
schema:url	https://doi.org/10.1111/j.1750-2659.2010.00149.x
resource representing a document's title	Original Article: Real time reverse transcription (RRT)‐polymerase chain reaction (PCR) methods for detection of pandemic (H1N1) 2009 influenza virus and European swine influenza A virus infections in pigs
has PubMed Central identifier	PMC4634650
has PubMed identifier	20716157
schema:publication	Influenza Other Respir Viruses
resource representing a document's body	covid:96cd30cbd4f3cecd2d0add83df270e1ae68670b1#body_text
is schema:about of	named entity 'METHODS' named entity 'SWINE INFLUENZA' named entity 'DIFFERENTIATE' named entity 'VIRUSES' named entity 'PCR' named entity 'METHODS' named entity 'REVERSE TRANSCRIPTION' named entity 'REQUIREMENT' named entity 'REAL TIME' named entity 'SWINE INFLUENZA' named entity 'EUROPEAN' named entity 'TO DETECT' named entity 'POLYMERASE CHAIN REACTION' named entity 'REVERSE TRANSCRIPTION' named entity 'REAL TIME' named entity 'INFLUENZA VIRUS' named entity 'PANDEMIC' named entity 'PIGS' named entity 'BACKGROUND' named entity 'IS A' named entity 'DETECTION' named entity 'INFLUENZA A VIRUS' named entity 'PANDEMIC' named entity 'INFECTIONS' named entity 'ESTABLISHED' named entity 'INFLUENZA A' covid:arg/96cd30cbd4f3cecd2d0add83df270e1ae68670b1 named entity 'reverse transcription' named entity 'swine influenza' named entity 'PCR' named entity 'H1N1' named entity 'influenza A virus' named entity 'influenza virus' named entity 'polymerase chain reaction' named entity 'virus isolation' named entity 'Avian influenza viruses' named entity 'electrophoresed' named entity 'nucleotide' named entity 'PCR' named entity 'influenza' named entity 'allantoic' named entity 'H1N2' named entity 'gene' named entity 'United Kingdom' named entity 'gene' named entity 'pig' named entity 'neuraminidase' named entity 'H1N1' named entity 'PCR' named entity 'gene' named entity 'influenza' named entity 'PCR' named entity 'agarose' named entity 'PCR' named entity 'influenza A virus' named entity 'OIE' named entity 'neuraminidase' named entity 'gene' named entity 'gene' named entity 'PCR' named entity 'gene' named entity 'centrifugation' named entity 'nucleotide' named entity 'RNA extraction' named entity 'gene' named entity 'gene' named entity 'H1N1'

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