About: A field‐deployable insulated isothermal RT‐PCR assay for identification of influenza A (H7N9) shows good performance in the laboratory

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About: A field‐deployable insulated isothermal RT‐PCR assay for identification of influenza A (H7N9) shows good performance in the laboratory Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A field‐deployable insulated isothermal RT‐PCR assay for identification of influenza A (H7N9) shows good performance in the laboratory
Creator	Guan, Yi Nguyen, Tung Inui, Ken Padungtod, Pawin Tsai, Yun-Long Chung, Simon Claes, Filip Kalpravidh, Wantanee Chuanfu, \| Huachen Zhu, \| Tsai, Mark Tseng, Hsin-Jou
topic	covid:96f0cf86f03bfe173c1b161ae5936757fb6a9b0e#this
Source	Medline; PMC
abstract	BACKGROUND: Avian influenza A (H7N9) remains circulating in China. For countries at risk of introduction of H7N9, such as Vietnam, early detection of H7N9 virus is essential for the early containment of the virus. Insulated isothermal reverse transcriptase PCR (iiRT‐PCR) is a portable PCR system that can be deployed under field conditions to identify pathogens at the sampling site. Applying PCR at the sampling site will greatly reduce the time to obtain a diagnostic result which allows the veterinary authority to take immediate action to contain disease spreading. OBJECTIVE: To determine analytical and diagnostic sensitivity and specificity of the portable iiRT‐PCR for H7N9 virus detection. METHODS: A panel of 59 virus isolates, including H7N9, avian influenza viruses of subtype H1 to H13, swine and human influenza viruses, Newcastle disease virus, and infectious bursal disease virus, were tested by H7 and N9 iiRT‐PCR reagents, using probes and primers specific to H7 or N9, in comparison with laboratory‐based real‐time RT‐PCR assays to determine analytical sensitivity and specificity. Fifty oropharyngeal samples from experimentally infected chicken and ducks with H7N9 and 50 non‐infected control swabs were tested by the H7 iiRT‐PCR to determine diagnostic sensitivity and specificity. RESULTS: The H7 and N9 iiRT‐PCR reagents yielded comparable levels of analytical sensitivity and specificity with real‐time RT‐PCR for the detection of H7N9 virus. Diagnostic sensitivity and specificity of H7 iiRT‐PCR were 98% and 100%, respectively. CONCLUSION: The observed high sensitivity and specificity of iiRT‐PCR for H7N9 detection show its potential for early detection of H7N9 in risk‐based surveillance.
has issue date	2019-09-05(xsd:dateTime)
bibo:doi	10.1111/irv.12646
bibo:pmid	31487118
has license	cc-by
sha1sum (hex)	96f0cf86f03bfe173c1b161ae5936757fb6a9b0e
schema:url	https://doi.org/10.1111/irv.12646
resource representing a document's title	A field‐deployable insulated isothermal RT‐PCR assay for identification of influenza A (H7N9) shows good performance in the laboratory
has PubMed Central identifier	PMC6800302
has PubMed identifier	31487118
schema:publication	Influenza Other Respir Viruses
resource representing a document's body	covid:96f0cf86f03bfe173c1b161ae5936757fb6a9b0e#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/96f0cf86f03bfe173c1b161ae5936757fb6a9b0e
is schema:about of	named entity 'performance' named entity 'REMAINS' named entity 'GOOD' named entity 'RT-PCR ASSAY' named entity 'ESSENTIAL' named entity 'SPREADING' named entity 'INFLUENZA A' named entity 'TO CONTAIN' named entity 'IDENTIFICATION' named entity 'H7N9' named entity 'APPLYING' named entity 'AT RISK' named entity 'GREATLY' named entity 'TO TAKE' named entity 'SYSTEM' named entity 'SAMPLING SITE' named entity 'DEPLOYED' named entity 'PATHOGENS' named entity 'COUNTRIES' named entity 'CONTAINMENT' named entity 'FIELD' named entity 'EARLY DETECTION' named entity 'TO IDENTIFY' named entity 'VETERINARY' named entity 'AVIAN INFLUENZA A' named entity 'PORTABLE' named entity 'PERFORMANCE' named entity 'DISEASE' named entity 'CHINA' named entity 'PCR' named entity 'FIELD' named entity 'ALLOWS' named entity 'VIRUS' named entity 'VIETNAM' named entity 'AUTHORITY' named entity 'IS A' named entity 'OBTAIN' named entity 'REDUCE' named entity 'TIME' named entity 'ACTION' named entity 'CONDITIONS' named entity 'CIRCULATING' named entity 'RISK OF' named entity 'BACKGROUND' named entity 'IMMEDIATE' named entity 'REVERSE TRANSCRIPTASE PCR' named entity 'INTRODUCTION' named entity 'LABORATORY' named entity 'EARLY' named entity 'site' named entity 'system' named entity 'PCR' named entity 'reverse transcriptase PCR' named entity 'H7N9' named entity 'pathogens' named entity 'China' named entity 'Avian influenza' named entity 'influenza' named entity 'Newcastle disease' named entity 'virus isolation' named entity 'nucleic acid' named entity 'PCR' named entity 'SPSS' named entity 'swine' named entity 'virus' named entity 'H7N9' named entity 'PCR' named entity 'sensitivity and specificity'

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