About: Production of virus-specific antiserum corresponding to sequences in the lactate dehydrogenase-elevating virus (LDV) ORF6 protein

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About: Production of virus-specific antiserum corresponding to sequences in the lactate dehydrogenase-elevating virus (LDV) ORF6 protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Production of virus-specific antiserum corresponding to sequences in the lactate dehydrogenase-elevating virus (LDV) ORF6 protein
Creator	Inada, T Kameoka, Y Matsushita, S Omoe, K Sakaguchi, M Takahashi-Omoe, H
Source	Elsevier; Medline; PMC
abstract	Abstract The elucidation of the antigenic structure of the envelope proteins of Arteriviridae which includes lactate dehydrogenase-elevating virus (LDV) will provide further understanding of a mechanism of strict host cell specificity. To analyze the linkage between LDV envelope proteins, M/VP-2 and VP-3, which may play an important role in viral infectivity, we generated specific antibody against M/VP-2 that has not been reported in previous studies. A synthetic polypeptide corresponding to the C-terminal region of LDV strain C (LDV-C) ORF6, which encodes M/VP-2, was chemically synthesized and coupled to keyhole limpet hemocyanin (KLH). The peptide was immunogenic in rabbits and induced antibody specific for viral protein. Western blotting and immunofluorescence analysis of virion M/VP-2 in infected macrophages showed that the antibody was able to react specifically with authentic virion protein. The immunoreactive antibody against LDV M/VP-2 described in this study will be useful for further studies of the specific roles of the envelope proteins in arterivirus assembly and infectivity.
has issue date	2004-01-31(xsd:dateTime)
bibo:doi	10.1016/s0147-9571(03)00035-3
bibo:pmid	14656541
has license	els-covid
sha1sum (hex)	96f8b2bdde4ca628a9915beee09b1ee3d5e35440
schema:url	https://doi.org/10.1016/s0147-9571%2803%2900035-3
resource representing a document's title	Production of virus-specific antiserum corresponding to sequences in the lactate dehydrogenase-elevating virus (LDV) ORF6 protein
has PubMed Central identifier	PMC7172777
has PubMed identifier	14656541
schema:publication	Comparative Immunology, Microbiology and Infectious Diseases
resource representing a document's body	covid:96f8b2bdde4ca628a9915beee09b1ee3d5e35440#body_text
is schema:about of	named entity 'infectivity' named entity 'les' named entity 'dans' named entity 'LDV' named entity 'immunofluorescence' named entity 'dans' named entity 'C-terminal' named entity 'virus' named entity 'virus' named entity 'specific' named entity 'protein' named entity 'cette' named entity 'VP-3' named entity 'specificity' named entity 'virus' named entity 'LDV' named entity 'play' named entity 'generated' named entity 'The' named entity 'Antiserum' named entity 'virus-specific' named entity 'virus' named entity 'infectivity' named entity 'immunogenic' named entity 'keyhole limpet hemocyanin' named entity 'KLH' named entity 'Western blotting' named entity 'arterivirus' named entity 'Arteriviridae' named entity 'infectivity' named entity 'lactate dehydrogenase-elevating virus' named entity 'KLH' named entity 'envelope proteins' named entity 'polypeptide' named entity 'antigenic' named entity 'virion' named entity 'antibody' named entity 'VP-2' named entity 'antibody' named entity 'chemically synthesized' named entity 'virus' named entity 'virus' named entity 'Arteriviridae' named entity 'lactate dehydrogenase-elevating virus' named entity 'Virus' named entity 'son' named entity 'molecular mass' named entity 'NaCl' named entity 'Buckinghamshire' named entity 'protein' named entity 'positive-stranded' named entity 'receptor' named entity 'mice' named entity 'recombinant' named entity 'amino acids' named entity 'immunization' named entity 'animal pathogens' named entity 'C-terminal' named entity 'virion' named entity 'PBS' named entity 'envelope protein' named entity 'kDa' named entity 'Roche Diagnostics' named entity 'paraformaldehyde' named entity 'cell lines' named entity 'protease inhibitor' named entity 'lactate dehydrogenase-elevating virus' named entity 'envelope proteins' named entity 'Carl Zeiss' named entity 'antibodies' named entity 'N-terminal end' named entity 'protein' named entity 'BSA' named entity 'antibodies' named entity 'mice' named entity 'antibody'

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