About: Effects of ribavirin on the replication and genetic stability of porcine reproductive and respiratory syndrome virus

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About: Effects of ribavirin on the replication and genetic stability of porcine reproductive and respiratory syndrome virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Effects of ribavirin on the replication and genetic stability of porcine reproductive and respiratory syndrome virus
Creator	Yoon, Kyoung-Jin Khatun, Amina Kim, Won-Il Shabir, Nadeem
topic	covid:9718efa7298b28b740938eeca415b1703ec6f689#this
Source	Medline; PMC
abstract	BACKGROUND: Although modified live virus (MLV) vaccines are commonly used for porcine reproductive and respiratory syndrome virus (PRRSV) control, there have been safety concerns due to the quick reversion of MLV to virulence during replication in pigs. Previous studies have demonstrated that mutant viruses emerged from lethal mutagenesis driven by antiviral mutagens and that those viruses had higher genetic stability compared to their parental strains because they acquired resistance to random mutation. Thus, this strategy was explored to stabilize the PRRSV genome in the current study. RESULTS: Four antiviral mutagens (ribavirin, 5-fluorouracil, 5-azacytidine, and amiloride) were evaluated for their antiviral effects against VR2332, a prototype of type 2 PRRSV. Among the mutagens, ribavirin and 5-fluorouracil had significant antiviral effects against VR2332. Consequently, VR2332 was serially passaged in MARC-145 cells in the presence of ribavirin at several concentrations to facilitate the emergence of ribavirin-resistant mutants. Two ribavirin-resistant mutants, RVRp13 and RVRp22, emerged from serial passages in the presence of 0.1 and 0.2 mM ribavirin, respectively. The genetic stability of these resistant mutants was evaluated in MARC-145 cells and compared with VR2332. As expected, the ribavirin-resistant mutants exhibited higher genetic stability compared to their parental virus. CONCLUSIONS: In summary, ribavirin and 5-fluorouracil effectively suppressed PRRSV replication in MARC-145 cells. However, ribavirin-resistant mutants emerged when treated with low concentrations (≤0.2 mM) of ribavirin, and those mutants were genetically more stable during serial passages in cell culture.
has issue date	2015-02-07(xsd:dateTime)
bibo:doi	10.1186/s12917-015-0330-z
bibo:pmid	25890207
has license	cc-by
sha1sum (hex)	9718efa7298b28b740938eeca415b1703ec6f689
schema:url	https://doi.org/10.1186/s12917-015-0330-z
resource representing a document's title	Effects of ribavirin on the replication and genetic stability of porcine reproductive and respiratory syndrome virus
has PubMed Central identifier	PMC4344762
has PubMed identifier	25890207
schema:publication	BMC Vet Res
resource representing a document's body	covid:9718efa7298b28b740938eeca415b1703ec6f689#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/9718efa7298b28b740938eeca415b1703ec6f689
is schema:about of	named entity 'genome' named entity 'fluorouracil' named entity 'viruses' named entity 'fluorouracil' covid:arg/9718efa7298b28b740938eeca415b1703ec6f689 named entity 'AMILORIDE' named entity 'VACCINES' named entity 'EMERGENCE' named entity 'USED' named entity 'RESISTANT' named entity 'MARC' named entity 'SUMMARY' named entity 'TREATED WITH' named entity '0.2' named entity 'RESULTS' named entity 'TYPE 2' named entity 'GENOME' named entity 'PROTOTYPE' named entity '5-AZACYTIDINE' named entity '5-FLUOROURACIL' named entity 'CURRENT' named entity 'TO STABILIZE' named entity 'A40' named entity '145' named entity 'SERIAL' named entity 'ACQUIRED' named entity 'DUE TO' named entity 'REVERSION' named entity 'STRATEGY' named entity 'MUTATION' named entity 'COMPARED' named entity 'LETHAL' named entity 'STRAINS' named entity 'STABLE' named entity 'HAVE' named entity 'CONCENTRATIONS' named entity 'EVALUATED' named entity 'SIGNIFICANT' named entity 'SUPPRESSED' named entity 'CELL CULTURE' named entity 'LOW' named entity 'CONCERNS' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'RANDOM' named entity 'VIRUS' named entity 'DEMONSTRATED' named entity 'ANTIVIRAL' named entity 'LIVE' named entity 'HIGHER' named entity '2 MM' named entity 'GENETIC STABILITY' named entity 'EFFECTS' named entity 'REPLICATION' named entity 'CONCLUSIONS' named entity 'VIRULENCE' named entity 'MUTANT' named entity 'QUICK' named entity 'SAFETY' named entity 'RIBAVIRIN' named entity 'CELLS' named entity 'STUDY' named entity 'DRIVEN' named entity 'EFFECTS' named entity 'MLV' named entity 'MUTAGENS' named entity 'STUDIES' named entity 'RESISTANCE TO' named entity 'AS EXPECTED' named entity 'PRESENCE OF' named entity 'PARENTAL' named entity 'THEIR' named entity 'THESE' named entity 'RIBAVIRIN' named entity 'MODIFIED' named entity 'PIGS' named entity 'PREVIOUS'

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