About: Evaluation of a multiplex immunoassay for bovine respiratory syncytial virus and bovine coronavirus antibodies in bulk tank milk against two indirect ELISAs using latent class analysis

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Evaluation of a multiplex immunoassay for bovine respiratory syncytial virus and bovine coronavirus antibodies in bulk tank milk against two indirect ELISAs using latent class analysis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of a multiplex immunoassay for bovine respiratory syncytial virus and bovine coronavirus antibodies in bulk tank milk against two indirect ELISAs using latent class analysis
Creator	Toft, Nils Stokstad, Maria Nødtvedt, Ane Toftaker, Ingrid Harkiss, Gordon Sølverød, Liv Watt, Neil O' Brien, Amanda
Source	Elsevier; Medline; PMC
abstract	Abstract Bovine respiratory syncytial virus (BRSV) and bovine coronavirus (BCV) are responsible for respiratory disease and diarrhea in cattle worldwide. The Norwegian control program against these infections is based on herd-level diagnosis using a new multiplex immunoassay. The objective of this study was to estimate sensitivity and specificity across different cut-off values for the MVD-Enferplex BCV/BRSV multiplex, by comparing them to a commercially available ELISA, the SVANOVIR® BCV-Ab and SVANOVIR® BRSV-Ab, respectively. We analyzed bulk tank milk samples from 360 herds in a low- and 360 herds in a high-prevalence area. As none of the tests were considered perfect, estimation of test characteristics was performed using Bayesian latent class models. At the manufacturers’ recommended cut-off values, the median sensitivity for the BRSV multiplex and the BRSV ELISA was 94.4 [89.8–98.7 95% Posterior Credibility Interval (PCI)] and 99.8 [98.7–100 95% PCI], respectively. The median specificity for the BRSV multiplex was 90.6 [85.5–94.4 95% PCI], but only 57.4 [50.5–64.4 95% PCI] for the BRSV ELISA. However, increasing the cut-off of the BRSV ELISA increased specificity without compromising sensitivity. For the BCV multiplex we found that by using only one of the three antigens included in the test, the specificity increased, without concurrent loss in sensitivity. At the recommended cut-off this resulted in a sensitivity of 99.9 [99.3–100 95% PCI] and specificity of 93.7 [88.8–97.8 95% PCI] for the multiplex and a sensitivity of 99.5 [98.1–100 95% PCI] and a specificity of 99.6 [97.6–100 95% PCI] for the BCV ELISA.
has issue date	2018-06-01(xsd:dateTime)
bibo:doi	10.1016/j.prevetmed.2018.03.008
bibo:pmid	29685432
has license	els-covid
sha1sum (hex)	9736738f64386aec2f45830b132f6012603a74ba
schema:url	https://doi.org/10.1016/j.prevetmed.2018.03.008
resource representing a document's title	Evaluation of a multiplex immunoassay for bovine respiratory syncytial virus and bovine coronavirus antibodies in bulk tank milk against two indirect ELISAs using latent class analysis
has PubMed Central identifier	PMC7114089
has PubMed identifier	29685432
schema:publication	Preventive Veterinary Medicine
resource representing a document's body	covid:9736738f64386aec2f45830b132f6012603a74ba#body_text
is schema:about of	named entity 'BOVINE RESPIRATORY SYNCYTIAL VIRUS' named entity 'INDIRECT' named entity 'based' named entity 'multiplex' named entity 'multiplex' named entity 'specificity' named entity 'Evaluation' named entity 'BOVINE CORONAVIRUS' named entity 'BULK' named entity 'ONE OF' named entity 'MILK' named entity 'MULTIPLEX' named entity 'CUT' named entity 'CHARACTERISTICS' named entity 'STUDY' named entity 'COMPARING' named entity 'AVAILABLE' named entity 'NORWEGIAN' named entity 'BULK' named entity 'ESTIMATE' named entity 'WORLDWIDE' named entity 'LOW' named entity 'INCREASING' named entity 'SAMPLES' named entity 'ESTIMATION' named entity 'PERFORMED' named entity 'RESPONSIBLE' named entity 'PREVALENCE' named entity 'ACROSS' named entity 'POSTERIOR' named entity 'MVD' named entity 'CONSIDERED' named entity 'DIARRHEA' named entity 'PCI' named entity 'BOVINE RESPIRATORY SYNCYTIAL VIRUS' named entity 'RESPIRATORY DISEASE' named entity 'USING' named entity 'CREDIBILITY' named entity 'BUT' named entity '28I' named entity 'IMMUNOASSAY' named entity 'SENSITIVITY' named entity 'LATENT CLASS MODELS' named entity 'MULTIPLEX' named entity 'BCV' named entity 'IMMUNOASSAY' named entity 'TANK' named entity 'BRSV' named entity 'MILK' named entity 'FOUND' named entity 'CONTROL' named entity 'INTERVAL' named entity 'BOVINE CORONAVIRUS' named entity 'ELISAS' named entity 'LATENT CLASS ANALYSIS' named entity 'USING' named entity 'ANTIBODIES' named entity 'LEVEL' named entity 'CATTLE' named entity 'AREA' named entity 'MEDIAN' named entity 'VETERINARY MEDICINE' named entity 'EVALUATION' named entity 'PREVENTIVE' named entity 'CONCURRENT' named entity '1-8' named entity '99.5' named entity 'RECOMMENDED' named entity 'PROGRAM' named entity 'NEW' named entity '360' named entity 'LOSS' named entity '100' named entity 'THESE'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software