About: Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2

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About: Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2
Creator	Yuan, Wanzhe Wang, Jinfeng Liu, Libing Wang, Jianchang Geng, Yunyun Li, Ruiwen Shi, Ruihan
Source	Elsevier; Medline; PMC
abstract	Abstract A visible and equipment-free recombinase polymerase amplification assay combined with a lateral flow strip (LFS RPA) was developed to detect canine parvovirus type 2 (CPV-2), which is the etiological agent of canine parvovirus disease. The CPV-2 LFS RPA assay was developed based on the VP2 gene and is performed in a closed fist using body heat for 15 min; the products are visible to the naked eye on the LFS within 5 min. The assay could detect CPV-2a, CPV-2b and CPV-2c, and there was no cross-reaction with the other viruses tested. Using the standard CPV-2 DNA as a template, the analytical sensitivity was 1.0 × 102 copies per reaction, which was the same result as that of a real-time PCR. The assay performance was further evaluated by testing 60 canine fecal samples, and CPV-2 DNA was detected in 46 samples (76.7%, 46/60) by LFS RPA, which was the same result as that of the real-time PCR assay and higher than that of the SNAP method (48.3%, 29/60). The novel CPV-2 LFS RPA assay is an attractive and promising tool for rapid and convenient diagnosis of CPV disease, especially cage side and in underequipped laboratories.
has issue date	2018-06-30(xsd:dateTime)
bibo:doi	10.1016/j.mcp.2018.04.004
bibo:pmid	29705183
has license	els-covid
sha1sum (hex)	97ff9997f9e7ad51bf2a7f0a6c6af01a52ec5007
schema:url	https://doi.org/10.1016/j.mcp.2018.04.004
resource representing a document's title	Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2
has PubMed Central identifier	PMC7127419
has PubMed identifier	29705183
schema:publication	Molecular and Cellular Probes
resource representing a document's body	covid:97ff9997f9e7ad51bf2a7f0a6c6af01a52ec5007#body_text
is schema:about of	named entity 'parvovirus' named entity 'visible' named entity 'DNA' named entity 'body heat' named entity 'assay' named entity 'result' named entity 'testing' named entity 'rapid' named entity 'PERFORMED' named entity 'STANDARD' named entity 'CPV' named entity 'REAL-TIME PCR ' named entity 'CONVENIENT' named entity 'ASSAY' named entity 'COPIES' named entity 'FREE' named entity 'EVALUATED' named entity 'TOOL' named entity 'PRODUCTS' named entity 'DETECT' named entity 'TESTING' named entity 'EYE' named entity 'VP2' named entity 'HIGHER' named entity 'GENE' named entity 'METHOD' named entity 'ANALYTICAL' named entity 'CANINE PARVOVIRUS' named entity 'SAMPLES' named entity 'TEMPLATE' named entity 'THE SNAP' named entity 'RESULT' named entity 'CLOSED' named entity 'DISEASE' named entity 'DIAGNOSIS' named entity 'ATTRACTIVE' named entity 'EQUIPMENT' named entity 'POLYMERASE' named entity 'HEAT' named entity 'FREE' named entity 'BASED' named entity 'NAKED' named entity 'RECOMBINASE' named entity 'VISUAL' named entity 'CANINE PARVOVIRUS' named entity 'DETECTION' named entity 'NEED' named entity 'SENSITIVITY' named entity 'VISIBLE' named entity 'TESTED' named entity 'RAPID' named entity 'BODY' named entity 'FIST' named entity 'HEAT' named entity 'TYPE 2' named entity 'FLOW' named entity 'NOVEL' named entity 'VIRUSES' named entity 'AGENT' named entity 'DNA' named entity 'VISIBLE TO' named entity 'FECAL' named entity 'MIN' named entity 'DETECTED' named entity 'LABORATORIES' named entity 'BODY' named entity 'CANINE' named entity 'RECOMBINASE' named entity 'POLYMERASE' named entity 'CAGE' named entity 'TO DETECT' named entity 'REACTION' named entity 'AMPLIFICATION' named entity 'LATERAL' named entity 'EQUIPMENT'

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