About: Gonadotropin Stimulation Increases the Expression of Angiotensin-(1

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About: Gonadotropin Stimulation Increases the Expression of Angiotensin-(1–7) and Mas Receptor in the Rat Ovary Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Gonadotropin Stimulation Increases the Expression of Angiotensin-(1–7) and Mas Receptor in the Rat Ovary
Creator	Santos, Robson Cassali, Geovanni Dos Reis, Adelina Honorato-Sampaio, Kinulpe Pereira, Virgínia Reis, Fernando Santos, Sérgio
topic	covid:98431e213477c506cd63f10992d4f7350efdc9e6#this
Source	PMC
abstract	We have previously shown the presence of immunoreactive angiotensin-(1–7) [Ang-(1–7)] in rat ovary homogenate and its stimulatory effect on estradiol and progesterone production in vitro. In the current study, we investigated the presence and cellular distribution of Ang-(1–7) and the Mas receptor, the expression of Mas and angiotensin-converting enzyme 2 (ACE2) messenger RNA (mRNA), and the enzymatic activity in the rat ovary following gonadotropin stimulation in vivo. Immature female Wistar rats (25 days old) were injected subcutaneously (SC) with equine chorionic gonadotropin (eCG, 20 IU in 0.2 mL) or vehicle 48 hours before euthanasia. Tissue distributions of Ang-(1–7), Mas receptor, and ACE2 were evaluated by immunohistochemistry, along with angiotensin II (Ang II) localization, while the mRNA expression levels of Mas receptor and ACE2 were evaluated by real-time polymerase chain reaction (PCR). In addition, we determined the activity of neutral endopeptidase (NEP), prolyl endopeptidase (PEP), and ACE by fluorometric assays. After eCG treatment, we found strong immunoreactivity for Ang-(1–7) and Mas primarily in the theca-interstitial cells, while Ang II appeared in the granulosa but not in the thecal layer. Equine chorionic gonadotropin treatment increased Mas and ACE2 mRNA expression compared with control animals (3.3- and 2.1-fold increase, respectively; P < .05). Angiotensin-converting enzyme and NEP activities were lower, while PEP activity was higher in the eCG-treated rats (P < .05). These data show gonadotropin-induced changes in the ovarian expression of Ang-(1–7), Mas receptor, and ACE2. These findings suggest that the renin-angiotensin system (RAS) branch formed by ACE2/Ang-(1–7)/Mas, fully expressed in the rat ovary and regulated by gonadotropic hormones, could play a role in the ovarian physiology.
has issue date	2009-12-30(xsd:dateTime)
bibo:doi	10.1177/1933719109343309
bibo:pmid	19703990
has license	no-cc
sha1sum (hex)	98431e213477c506cd63f10992d4f7350efdc9e6
schema:url	https://doi.org/10.1177/1933719109343309
resource representing a document's title	Gonadotropin Stimulation Increases the Expression of Angiotensin-(1–7) and Mas Receptor in the Rat Ovary
has PubMed Central identifier	PMC7101720
has PubMed identifier	19703990
schema:publication	Reprod Sci
resource representing a document's body	covid:98431e213477c506cd63f10992d4f7350efdc9e6#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/98431e213477c506cd63f10992d4f7350efdc9e6
is schema:about of	named entity 'Tissue' named entity 'angiotensin' named entity 'After' named entity 'expression' named entity 'GONADOTROPIC HORMONES' named entity 'HAVE' named entity 'DISTRIBUTIONS' named entity 'gonadotropic' named entity 'days' named entity 'ovary' named entity 'ACE2' named entity 'localization' named entity 'activity' named entity 'homogenate' named entity 'immunoreactivity' named entity 'injected' named entity 'ovarian' named entity 'eCG' named entity 'immunoreactive' named entity 'Mas' named entity 'rats' named entity 'ovary' named entity 'PCR' named entity 'Stimulation' named entity 'Angiotensin' named entity 'mRNA' named entity 'immunoreactivity' named entity 'ovary' named entity 'angiotensin' named entity 'gonadotropic hormones' named entity 'ACE2' named entity 'ACE2' named entity 'rat' named entity 'prolyl endopeptidase' named entity 'subcutaneously' named entity 'ACE2' named entity 'ovary' named entity 'immunohistochemistry' named entity 'Gonadotropin' named entity 'immunohistochemistry' named entity 'methanol' named entity 'rat' named entity 'Ang II' named entity 'endoplasmic reticulum' named entity 'isopropanol' named entity 'eCG' named entity 'liquid nitrogen' named entity 'angiotensinogen' named entity 'ovaries' named entity 'laparotomy' named entity 'serum' named entity 'mol/L' named entity '65 nm' named entity 'rat' named entity 'mmol/L' named entity 'California' named entity 'alcohols' named entity 'androgen' named entity 'RAS' named entity 'ACE2' named entity 'ovary' named entity 'Prorenin' named entity '2.1' named entity 'biologically active' named entity 'antral' named entity 'ovary' named entity 'substrate' named entity 'gonadotropin' named entity 'Negative control' named entity 'mRNA' named entity 'ovary' named entity 'homogenate' named entity 'mmol/L'

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