About: Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner

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About: Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner
Creator	Herrler, Georg Huang, Yao-Wei Meng, Fandan Tang, Yan-Dong Yang, Yue-Lin
Source	Medline; PMC
abstract	Porcine deltacoronavirus (PDCoV) is a newly emerging threat to the global porcine industry. PDCoV has been successfully isolated using various medium additives including trypsin, and although we know it is important for viral replication, the mechanism has not been fully elucidated. Here, we systematically investigated the role of trypsin in PDCoV replication including cell entry, cell-to-cell membrane fusion and virus release. Using pseudovirus entry assays, we demonstrated that PDCoV entry is not trypsin dependent. Furthermore, unlike porcine epidemic diarrhea virus (PEDV), in which trypsin is important for the release of virus from infected cells, PDCoV release was not affected by trypsin. We also demonstrated that trypsin promotes PDCoV replication by enhancing cell-to-cell membrane fusion. Most importantly, our study illustrates two distinct spreading patterns from infected cells to uninfected cells during PDCoV transmission, and the role of trypsin in PDCoV replication in cells with different virus spreading types. Overall, these results clarify that trypsin promotes PDCoV replication by mediating cell-to-cell fusion transmission but is not crucial for viral entry. This knowledge can potentially contribute to improvement of virus production efficiency in culture, not only for vaccine preparation but also to develop antiviral treatments.
has issue date	2020-02-24(xsd:dateTime)
bibo:doi	10.1080/22221751.2020.1730245
bibo:pmid	32090689
has license	cc-by
sha1sum (hex)	98a53ab5c12da91e1a7ff02c6df62ac9fc85ce41
schema:url	https://doi.org/10.1080/22221751.2020.1730245
resource representing a document's title	Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner
has PubMed Central identifier	PMC7054919
has PubMed identifier	32090689
schema:publication	Emerg Microbes Infect
resource representing a document's body	covid:98a53ab5c12da91e1a7ff02c6df62ac9fc85ce41#body_text
is schema:about of	named entity 'CELL TYPE' named entity 'This' named entity 'clarify' named entity 'deltacoronavirus' named entity 'A CELL' named entity 'DEPENDENT' named entity 'FULLY' named entity 'VIRUS RELEASE' covid:arg/98a53ab5c12da91e1a7ff02c6df62ac9fc85ce41 named entity 'THESE' named entity 'VIRUS PRODUCTION' named entity 'CULTURE' named entity 'CELL' named entity 'KNOWLEDGE' named entity 'CELL' named entity 'DEPENDENT' named entity 'UNINFECTED' named entity 'ANTIVIRAL' named entity 'THREAT' named entity 'EFFICIENCY' named entity 'VIRUS' named entity 'INCLUDING' named entity 'MEDIUM' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'PATTERNS' named entity 'MEDIATING' named entity 'PORCINE DELTACORONAVIRUS' named entity 'CELL FUSION' named entity 'ENHANCING' named entity 'VARIOUS' named entity 'PSEUDOVIRUS' named entity 'TRYPSIN' named entity 'ENTRY' named entity '2C CELL' named entity 'TRANSMISSION' named entity 'DISTINCT' named entity 'DEMONSTRATED' named entity 'REPLICATION' named entity 'VIRAL ENTRY' named entity 'INFECTED' named entity 'GLOBAL' named entity 'ISOLATED' named entity 'IS A' named entity 'USING' named entity 'ROLE' named entity 'STUDY' named entity 'CELL FUSION' named entity 'SPREADING' named entity 'DIFFERENT' named entity 'OUR' named entity 'AFFECTED' named entity 'MECHANISM' named entity 'RELEASE' named entity 'IMPORTANT' named entity 'CELL MEMBRANE' named entity 'PORCINE DELTACORONAVIRUS' named entity 'IMPROVEMENT' named entity 'NEWLY' named entity 'CELLS' named entity 'HERE' named entity 'MEMBRANE FUSION' named entity 'ADDITIVES' named entity 'MEDIATING' named entity 'CLARIFY' named entity 'RESULTS' named entity 'TREATMENTS' named entity 'TYPES' named entity 'VACCINE PREPARATION' named entity 'INDUSTRY' named entity 'VIRAL REPLICATION' named entity 'BUT' named entity 'PORCINE' named entity 'INVESTIGATED' named entity 'TRYPSIN' named entity 'CONTRIBUTE ' named entity 'OVERALL' named entity 'types'

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