About: Loop‐mediated isothermal amplification (LAMP): a versatile technique for detection of micro‐organisms

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About: Loop‐mediated isothermal amplification (LAMP): a versatile technique for detection of micro‐organisms Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Loop‐mediated isothermal amplification (LAMP): a versatile technique for detection of micro‐organisms
Creator	Chee, Lau, Y.-L Chee, H.-Y Correspondence, Hui-Yee Othman, S Othman, Shuhaidah Radu, S Wong, Y.-P Wong, Yien-Ping
topic	covid:99895776954fb65d8341f3f383e35e9951b4a798#this
Source	Medline; PMC
abstract	Loop‐mediated isothermal amplification (LAMP) amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions by using a DNA polymerase with high displacement strand activity and a set of specifically designed primers to amplify targeted DNA strands. Following its first discovery by Notomi et al. (2000 Nucleic Acids Res 28: E63), LAMP was further developed over the years which involved the combination of this technique with other molecular approaches, such as reverse transcription and multiplex amplification for the detection of infectious diseases caused by micro‐organisms in humans, livestock and plants. In this review, available types of LAMP techniques will be discussed together with their applications in detection of various micro‐organisms. Up to date, there are varieties of LAMP detection methods available including colorimetric and fluorescent detection, real‐time monitoring using turbidity metre and detection using lateral flow device which will also be highlighted in this review. Apart from that, commercialization of LAMP technique had also been reported such as lyophilized form of LAMP reagents kit and LAMP primer sets for detection of pathogenic micro‐organisms. On top of that, advantages and limitations of this molecular detection method are also described together with its future potential as a diagnostic method for infectious disease.
has issue date	2018-02-12(xsd:dateTime)
bibo:doi	10.1111/jam.13647
bibo:pmid	29165905
has license	no-cc
sha1sum (hex)	99895776954fb65d8341f3f383e35e9951b4a798
schema:url	https://doi.org/10.1111/jam.13647
resource representing a document's title	Loop‐mediated isothermal amplification (LAMP): a versatile technique for detection of micro‐organisms
has PubMed Central identifier	PMC7167136
has PubMed identifier	29165905
schema:publication	J Appl Microbiol
resource representing a document's body	covid:99895776954fb65d8341f3f383e35e9951b4a798#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/99895776954fb65d8341f3f383e35e9951b4a798
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