About: Identification by functional proteomics of a deubiquitinating/deNeddylating enzyme in Plasmodium falciparum

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About: Identification by functional proteomics of a deubiquitinating/deNeddylating enzyme in Plasmodium falciparum Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification by functional proteomics of a deubiquitinating/deNeddylating enzyme in Plasmodium falciparum
Creator	Misaghi, Shahram Spooner, Eric Ploegh, Hidde Artavanis-Tsakonas, Katerina Catic, Andre Comeaux, Christy Duraisingh, Manoj
Source	Medline; PMC
abstract	Ubiquitination is a post‐translational modification implicated in a variety of cellular functions, including transcriptional regulation, protein degradation and membrane protein trafficking. Ubiquitin and the enzymes that act on it, although conserved and essential in eukaryotes, have not been well studied in parasites, despite sequencing of several parasite genomes. Several putative ubiquitin hydrolases have been identified in Plasmodium falciparum based on sequence homology alone, with no evidence of expression or function. Here we identify the first deubiquitinating enzyme in P. falciparum, PfUCH54, by its activity. We show that PfUCH54 also has deNeddylating activity, as assayed by a mammalian Nedd8‐based probe. This activity is absent from mammalian homologues of PfUCH54. Given the importance of parasitic membrane protein trafficking as well as protein degradation in the virulence of this parasite, this family of enzymes may represent a target for pharmacological intervention with this disease.
has issue date	2006-08-02(xsd:dateTime)
bibo:doi	10.1111/j.1365-2958.2006.05307.x
bibo:pmid	16925553
has license	no-cc
sha1sum (hex)	99e63a644c29960da637bec6ecb8bbfc78a98041
schema:url	https://doi.org/10.1111/j.1365-2958.2006.05307.x
resource representing a document's title	Identification by functional proteomics of a deubiquitinating/deNeddylating enzyme in Plasmodium falciparum
has PubMed Central identifier	PMC7168409
has PubMed identifier	16925553
schema:publication	Mol Microbiol
resource representing a document's body	covid:99e63a644c29960da637bec6ecb8bbfc78a98041#body_text
is schema:about of	named entity 'FAMILY' named entity 'UBIQUITIN' named entity 'TARGET' covid:arg/99e63a644c29960da637bec6ecb8bbfc78a98041 named entity 'ubiquitin' named entity 'parasite' named entity 'Plasmodium falciparum' named entity 'post-translational modification' named entity 'parasitic' named entity 'eukaryotes' named entity 'hydrolases' named entity 'Plasmodium falciparum' named entity 'deubiquitinating' named entity 'functional proteomics' named entity 'sequence identity' named entity 'Protein-A' named entity 'parasite' named entity 'enzyme' named entity 'cloning' named entity 'morbidity' named entity 'SUMO' named entity 'cross-reaction' named entity 'protein' named entity 'UCH37' named entity 'peptide' named entity 'flash frozen' named entity 'UCH37' named entity 'parasite' named entity 'trypsinized' named entity 'cysteine' named entity 'Boston, MA' named entity 'immunoblotting' named entity 'UCH37' named entity 'parasites' named entity 'deubiquitinating' named entity 'parasites' named entity 'apoptosis' named entity 'active site' named entity 'ISG15' named entity 'UL36' named entity 'cytoplasm' named entity 'drug target' named entity 'epitope tag' named entity 'reticulocyte' named entity 'MS/MS' named entity 'St Louis' named entity 'deubiquitinating' named entity 'P. falciparum' named entity 'highly conserved sequence' named entity 'polypeptide' named entity 'P. falciparum' named entity 'mechanisms of action' named entity 'transcription' named entity 'ISG15' named entity 'conjugation' named entity 'pH 7' named entity 'adduct' named entity 'cell cycle control' named entity 'cysteine' named entity 'homopolymeric' named entity 'cellular processes' named entity 'lysate' named entity 'Plasmodium' named entity 'DpnI' named entity 'sequence similarity' named entity 'protein' named entity 'Nedd8' named entity 'lysis buffer' named entity 'Nedd8' named entity 'deubiquitinating' named entity 'NaCl' named entity 'protein' named entity 'parasitic' named entity 'cysteine' named entity 'pathogenicity'

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