About: A Reverse Genetics Platform That Spans the Zika Virus Family Tree

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Reverse Genetics Platform That Spans the Zika Virus Family Tree
Creator	Baric, Ralph Yount, Boyd Gallichotte, Emily Swanstrom, Jesica Heise, Mark Plante, Jessica Young, Ellen Carbaugh, Derek Lazear, Helen Peck, Kayla Plante, Kenneth Widman, Douglas
Source	Medline; PMC
abstract	Zika virus (ZIKV), a mosquito-borne flavivirus discovered in 1947, has only recently caused large outbreaks and emerged as a significant human pathogen. In 2015, ZIKV was detected in Brazil, and the resulting epidemic has spread throughout the Western Hemisphere. Severe complications from ZIKV infection include neurological disorders such as Guillain-Barré syndrome in adults and a variety of fetal abnormalities, including microcephaly, blindness, placental insufficiency, and fetal demise. There is an urgent need for tools and reagents to study the pathogenesis of epidemic ZIKV and for testing vaccines and antivirals. Using a reverse genetics platform, we generated six ZIKV infectious clones and derivative viruses representing diverse temporal and geographic origins. These include three versions of MR766, the prototype 1947 strain (with and without a glycosylation site in the envelope protein), and H/PF/2013, a 2013 human isolate from French Polynesia representative of the virus introduced to Brazil. In the course of synthesizing a clone of a circulating Brazilian strain, phylogenetic studies identified two distinct ZIKV clades in Brazil. We reconstructed viable clones of strains SPH2015 and BeH819015, representing ancestral members of each clade. We assessed recombinant virus replication, binding to monoclonal antibodies, and virulence in mice. This panel of molecular clones and recombinant virus isolates will enable targeted studies of viral determinants of pathogenesis, adaptation, and evolution, as well as the rational attenuation of contemporary outbreak strains to facilitate the design of vaccines and therapeutics.
has issue date	2017-03-07(xsd:dateTime)
bibo:doi	10.1128/mbio.02014-16
bibo:pmid	28270583
has license	cc-by
sha1sum (hex)	99f6ca41bcf6a11e4a3c722d5e00a9bfec79ca19
schema:url	https://doi.org/10.1128/mbio.02014-16
resource representing a document's title	A Reverse Genetics Platform That Spans the Zika Virus Family Tree
has PubMed Central identifier	PMC5340872
has PubMed identifier	28270583
schema:publication	mBio
resource representing a document's body	covid:99f6ca41bcf6a11e4a3c722d5e00a9bfec79ca19#body_text
is schema:about of	named entity 'antivirals' named entity 'recombinant virus' named entity 'targeted' named entity 'Brazilian' named entity 'identified' named entity 'large' named entity 'ZIKA VIRUS' named entity 'DERIVATIVE' named entity 'EPIDEMIC' named entity 'REAGENTS' named entity 'STUDIES' named entity 'PLATFORM' named entity 'DETECTED' named entity 'ASSESSED' named entity 'TEMPORAL' named entity 'ENABLE' named entity 'RECOMBINANT VIRUS' named entity 'BINDING' named entity 'MOLECULAR' named entity 'MICE' named entity 'GENERATED' named entity 'SEVERE' named entity 'VIRULENCE' named entity 'ENVELOPE PROTEIN' named entity 'GEOGRAPHIC' named entity 'BLINDNESS' named entity 'ANTIVIRALS' named entity 'URGENT' named entity 'ADAPTATION' named entity 'REVERSE GENETICS' named entity 'HUMAN' named entity '2015' named entity 'CLONE' named entity 'RESULTING' named entity 'PROTOTYPE' named entity 'DESIGN' named entity 'PLATFORM' named entity 'THESE' named entity 'ADULTS' named entity 'CLADE' named entity 'EVOLUTION' named entity 'ZIKA VIRUS' named entity 'INCLUDE' named entity 'VACCINES' named entity 'USING' named entity 'FETAL DEMISE' named entity 'FETAL ABNORMALITIES' named entity 'VIRUS REPLICATION' named entity 'REPRESENTATIVE' named entity 'LARGE' named entity 'COURSE' named entity 'THERAPEUTICS' named entity 'MOSQUITO' named entity 'FRENCH POLYNESIA' named entity 'DISTINCT' named entity 'VIABLE' named entity 'TOOLS' named entity 'VIRAL' named entity 'BARR' named entity 'OUTBREAK' named entity 'PATHOGEN' named entity 'NEUROLOGICAL DISORDERS' named entity 'ORIGINS' named entity 'PLACENTAL INSUFFICIENCY' named entity 'INFECTIOUS' named entity 'COMPLICATIONS' named entity 'INTRODUCED' named entity 'IDENTIFIED' named entity 'SYNDROME' named entity 'PANEL'

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