About: Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses
Creator	Shadab, Azadeh Yavarian, Jila Naseri, Maryam Eshragian, Mohammadreza Sultani, Mozhdeh Azad, Mokhtari Owrang Eilami, ; Talat, ; Yavarian, ;
source	Medline; PMC
abstract	BACKGROUND: It is often difficult for a physician to distinguish between viral and bacterial causes of respiratory infections and this may result in overuse of antibiotics. In many cases of community-acquired respiratory infections, clinicians treat patients empirically. The development of molecular methods for direct detection of viruses has been progressed recently. OBJECTIVES: The objective of this study was recognizing the panel of respiratory RNA viruses by multiplex SYBR Green real-time polymerase chain reaction (PCR). MATERIALS AND METHODS: Randomized 172 influenza-negative respiratory specimens of all age groups of hospitalized patients were collected. After RNA extraction, cDNA was synthesized. Three SYBR Green multiplex real-time PCR assays were developed for simultaneous detection of 12 respiratory RNA viruses. Each set of multiplex methods detected four viruses, the first set: respiratory syncytial virus, human metapneumovirus, rhinovirus, enterovirus; the second set: parainfluenza viruses 1 - 4 (PIV1-4); the third set: coronaviruses NL63, 229E, severe acute respiratory syndrome (SARS), and OC43. RESULTS: Application of the multiplex SYBR Green real-time PCR in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) PIV3, 9 (5.23%) PIV4, and 1 (0.58%) coronavirus NL63. All the positive samples were detected during December to March (2011 - 2012). CONCLUSIONS: Multiplex SYBR Green real-time PCR is a rapid and relatively inexpensive method for detection of respiratory viruses.
has issue date	2015-08-01(xsd:dateTime)
bibo:doi	10.5812/jjm.19041v2
bibo:pmid	26468358
has license	cc-by-nc
sha1sum (hex)	9a0f63b592a07e5823715e548da6f3605faf2bbb
schema:url	https://doi.org/10.5812/jjm.19041v2
resource representing a document's title	Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses
has PubMed Central identifier	PMC4601230
has PubMed identifier	26468358
schema:publication	Jundishapur J Microbiol
resource representing a document's body	covid:9a0f63b592a07e5823715e548da6f3605faf2bbb#body_text
is schema:about of	named entity 'SYBR Green' named entity 'Viruses' named entity 'GREEN ' named entity 'MULTIPLEX' named entity 'DETECTION' named entity 'SYBR GREEN' named entity 'PCR ASSAY' named entity 'RESPIRATORY VIRUSES' named entity 'REAL-TIME PCR ' named entity 'bronchiolitis' named entity 'coronavirus' named entity 'epidemics' named entity 'gene' named entity 'standard curve' named entity 'reverse transcription' named entity 'real-time PCR' named entity 'PCR' named entity 'RSV' named entity 'HMPV' named entity 'HMPV' named entity 'SYBR Green' named entity 'serology' named entity 'respiratory infections' named entity 'Germany' named entity 'human metapneumovirus' named entity 'antibiotics' named entity 'RSV' named entity 'SARS' named entity 'rhinorrhea' named entity 'primers' named entity 'real-time PCR' named entity 'Real-Time PCR' named entity 'HMPV' named entity 'immunofluorescent' named entity 'cDNA' named entity 'Acute respiratory infections' named entity 'coronavirus' named entity 'bacterial infections' named entity 'temperate climates' named entity 'etiology' named entity 'ARIs' named entity 'Taqman' named entity 'viruses' named entity 'viruses' named entity 'pediatric' named entity 'PCR' named entity 'viruses' named entity 'sensitivity and specificity' named entity 'viral infections' named entity 'HMPV' named entity 'pneumonia' named entity 'respiratory diseases' named entity 'respiratory tract infections' named entity 'cough' named entity 'nmol' named entity 'Picornaviridae' named entity 'Iran' named entity 'ILI' named entity 'coronavirus' named entity 'PCR' named entity 'RSV' named entity 'Reverse transcription' named entity 'denaturation' named entity 'rhinovirus' named entity 'type 68' named entity 'World Health Organization' named entity 'RNA' named entity 'young children' named entity 'viruses' named entity 'influenza viruses' named entity 'viral culture' named entity 'PCR' named entity 'influenza-like'

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