About: A monoclonal antibody binds to threonine 49 in the non-structural 1 protein of influenza A virus and interferes with its ability to modulate viral replication

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About: A monoclonal antibody binds to threonine 49 in the non-structural 1 protein of influenza A virus and interferes with its ability to modulate viral replication Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A monoclonal antibody binds to threonine 49 in the non-structural 1 protein of influenza A virus and interferes with its ability to modulate viral replication
Creator	Tan, Yee-Joo Song, Jianxing Barnwal, Bhaskar Zeng, Qi Gupta, Garvita Mok, Chee-Keng Wu, Jianping Chow, Kwong Tak, Vincent Kedar Diwakar, Mandakhalikar Yuan, Y
Source	Elsevier; Medline; PMC
abstract	Abstract The emergence of resistant influenza A viruses highlights the continuous requirement of new antiviral drugs that can treat the viral infection. Non-structural 1 (NS1) protein, an indispensable component for efficient virus replication, can be used as a potential target for generating new antiviral agents. Here, we study the interaction of 2H6 monoclonal antibody with NS1 protein and also determine whether influenza virus replication can be inhibited by blocking NS1. The 2H6-antigen binding fragment (Fab) forms a multimeric complex with the NS1 RNA-binding domain (RBD). T49, a residue which forms a direct hydrogen bond with double stranded RNA, in NS1 protein was found to be critical for its interaction with 2H6 antibody. NS1(RBD) has high affinity to 2H6 with KD of 43.5±4.24nM whereas NS1(RBD)-T49A has more than 250 times lower affinity towards 2H6. Interestingly, the intracellular expression of 2H6-single-chain variable fragment (scFv) in mammalian cells caused a reduction in viral growth and the M1 viral protein level was significantly reduced in 2H6-scFv transfected cells in comparison to vector transfected cells at 12h post infection. These results indicate that the tight binding of 2H6 to NS1 could lead to reduction in viral replication and release of progeny virus. In future, 2H6 antibody in combination with other neutralizing antibodies can be used to increase the potency of viral inhibition.
has issue date	2015-04-30(xsd:dateTime)
bibo:doi	10.1016/j.antiviral.2015.01.015
bibo:pmid	25666762
has license	els-covid
sha1sum (hex)	9b2d1c90b6e6dc56b7d95942902cb1e5057d49dc
schema:url	https://doi.org/10.1016/j.antiviral.2015.01.015
resource representing a document's title	A monoclonal antibody binds to threonine 49 in the non-structural 1 protein of influenza A virus and interferes with its ability to modulate viral replication
has PubMed Central identifier	PMC7113856
has PubMed identifier	25666762
schema:publication	Antiviral Research
resource representing a document's body	covid:9b2d1c90b6e6dc56b7d95942902cb1e5057d49dc#body_text
is schema:about of	named entity 'direct' named entity 'scFv' named entity 'binds' named entity 'ANTIGEN BINDING FRAGMENT' named entity 'INHIBITED' named entity 'HERE' named entity 'ANTIBODY' named entity 'INFECTION' named entity 'RESULTS' named entity 'AFFINITY' named entity 'POTENCY' named entity 'VIRAL PROTEIN' named entity 'MONOCLONAL ANTIBODY' named entity 'BINDING' named entity 'INTERACTION' named entity 'INHIBITION' named entity 'LEAD' named entity 'VIRAL REPLICATION' named entity 'TO INCREASE' named entity 'DETERMINE' named entity 'PROTEIN' named entity 'TIMES' named entity 'FORMS' named entity 'MONOCLONAL ANTIBODY' named entity 'EFFICIENT' named entity 'EMERGENCE' named entity 'POST' named entity 'NS1' named entity 'COMPONENT' named entity 'BINDS' named entity 'INTERFERES WITH' named entity 'THREONINE' named entity 'INFLUENZA A VIRUS' named entity 'STRUCTURAL' named entity 'MODULATE' named entity 'NON-' named entity 'ITS' named entity 'NEW' named entity 'INTRACELLULAR' named entity 'VIRUS' named entity 'TIGHT' named entity 'NEUTRALIZING ANTIBODIES' named entity 'CAUSED' named entity 'VIRUS REPLICATION' named entity 'TREAT' named entity 'T49' named entity 'REQUIREMENT' named entity 'VIRUSES' named entity 'EXPRESSION' named entity 'VIRAL INFECTION' named entity 'RESIDUE' named entity 'POTENTIAL' named entity 'PROGENY' named entity 'ANTIVIRAL AGENTS' named entity 'DOUBLE STRANDED RNA' named entity 'PROTEIN ' named entity 'NS1 PROTEIN' named entity 'ITS' named entity '250' named entity 'RESISTANT' named entity 'DIRECT' named entity 'CHAIN' named entity 'STUDY' named entity 'GROWTH' named entity 'INFLUENZA A' named entity 'LOWER' named entity 'THESE' named entity 'VIRAL REPLICATION' named entity 'PROTEIN ' named entity 'VIRAL' named entity 'VARIABLE'

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