About: Immunogenicity of a neutralizing epitope from porcine epidemic diarrhea virus: M cell targeting ligand fusion protein expressed in transgenic rice calli

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About: Immunogenicity of a neutralizing epitope from porcine epidemic diarrhea virus: M cell targeting ligand fusion protein expressed in transgenic rice calli Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Immunogenicity of a neutralizing epitope from porcine epidemic diarrhea virus: M cell targeting ligand fusion protein expressed in transgenic rice calli
Creator	Yang, Moon-Sik Kim, Sae-Hae Huy, Nguyen-Xuan Kim, Tae-Geum
Source	Medline; PMC
abstract	To increase immune responses of plant-based vaccines in intestine mucosal immune systems, a synthetic neutralizing epitope (sCOE) gene of porcine epidemic diarrhea virus (PEDV) was fused with M cell-targeting ligand (Co1) and introduced into a plant expression vector under the control of rice amylase 3D promoter. The sCOE–Co1 fusion gene was introduced into rice calli via the particle bombardment-mediated transformation method. The stable integration and transcriptional expression of the sCOE–Co1 fusion gene was confirmed by genomic DNA PCR amplification and Northern blot analysis, respectively. The expression of the COE–Co1 fusion protein was confirmed by immunoblot analysis. The highest expression level of the COE–Co1 fusion protein reached 0.083 % of the total soluble protein according to quantitative densitometry of Western blot analysis. Mice immunized with transgenic rice calli protein extracts induced significant serum IgG and fecal IgA antibody levels against purified bacterial COE. The systemic and mucosal immune responses were confirmed by measuring COE-specific IgG and IgA antibody-secreting cells in the lymphocytes extracted from the spleen and COE-specific IgA antibody-secreting cells in the Peyer’s patches from immunized mice. These results indicated that oral immunization of plant-produced COE–Co1 fusion protein could elicit efficient systemic and mucosal immune responses against the COE antigen. Key message Neutralizing epitope from porcine epidemic diarrhea virus-M cell targeting ligand fusion protein was produced in transgenic rice calli and elicited systemic and mucosal immune responses by oral administration in mice.
has issue date	2012-06-27(xsd:dateTime)
bibo:doi	10.1007/s00299-012-1306-0
bibo:pmid	22736145
has license	no-cc
sha1sum (hex)	9b45430d80c764f7ed43dfb58729b6b3a8c661dc
schema:url	https://doi.org/10.1007/s00299-012-1306-0
resource representing a document's title	Immunogenicity of a neutralizing epitope from porcine epidemic diarrhea virus: M cell targeting ligand fusion protein expressed in transgenic rice calli
has PubMed Central identifier	PMC7080027
has PubMed identifier	22736145
schema:publication	Plant Cell Rep
resource representing a document's body	covid:9b45430d80c764f7ed43dfb58729b6b3a8c661dc#body_text
is schema:about of	named entity 'PROTEIN ' named entity 'cells' named entity 'Northern blot' named entity 'transformation method' named entity 'immunoblot' named entity 'mice' named entity 'integration' named entity 'IMMUNIZED' named entity 'INTRODUCED INTO' named entity 'BASED' named entity 'ANTIGEN' named entity 'EXPRESSION LEVEL' named entity 'FECAL' named entity 'BACTERIAL' named entity 'TRANSCRIPTIONAL' named entity 'INDUCED' named entity 'HIGHEST' named entity 'IMMUNE RESPONSES' named entity 'SPECIFIC' named entity 'MICE' named entity 'RESULTS' named entity 'PLANT' named entity 'QUANTITATIVE' named entity 'ORAL IMMUNIZATION' named entity 'COE' covid:arg/9b45430d80c764f7ed43dfb58729b6b3a8c661dc named entity 'EXPRESSED' named entity 'SECRETING' named entity 'SIGNIFICANT' named entity 'ORAL ADMINISTRATION' named entity 'CONFIRMED BY' named entity 'FUSED WITH' named entity 'M CELL' named entity 'EFFICIENT' named entity 'INTESTINE' named entity 'FUSION GENE' named entity 'TRANSFORMATION' named entity 'NORTHERN BLOT ANALYSIS' named entity 'SERUM' named entity 'PARTICLE' named entity 'PROTEIN ' named entity 'TARGETING LIGAND' named entity 'DENSITOMETRY' named entity 'MUCOSAL IMMUNE SYSTEMS' named entity 'KEY' named entity 'CONTROL' named entity 'M CELL' named entity 'VACCINES' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'SOLUBLE PROTEIN' named entity 'EXTRACTED' named entity 'RICE' named entity 'METHOD' named entity 'TO INCREASE' named entity 'INTEGRATION' named entity 'PROMOTER' named entity 'LYMPHOCYTES' named entity 'LEVELS' named entity 'PLANT-' named entity 'TRANSGENIC RICE' named entity 'IGG' named entity 'AMYLASE' named entity 'MEDIATED' named entity 'FUSION PROTEIN' named entity 'IMMUNOGENICITY' named entity 'FUSION PROTEIN' named entity 'EPITOPE' named entity 'TRANSGENIC RICE' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'MESSAGE' named entity '27S' named entity 'IMMUNOBLOT ANALYSIS' named entity 'WESTERN BLOT ANALYSIS' named entity 'INDICATED' named entity 'THESE' named entity 'IGA ANTIBODY' named entity 'EXPRESSION' named entity 'TOTAL'

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