About: A recombinant single chain antibody neutralizes coronavirus infectivity but only slightly delays lethal infection of mice

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A recombinant single chain antibody neutralizes coronavirus infectivity but only slightly delays lethal infection of mice
Creator	Lamarre, Alain Chagnon, Fanny Talbot, Pierre Yu, Mathilde
source	Medline; PMC
abstract	The variable region genes of a murine anti‐coronavirus monoclonal antibody (mAb) were joined by assembly polymerase chain reaction and expressed in Escherichia coli in a single chain variable fragment (scFv) configuration. After induction of expression, the expected 32‐kDa protein was identified by Western immunoblotting with specific rabbit anti‐idiotype antibodies. The scFv fragments were purified from soluble cytoplasmic preparations by affinity chromatography on nickel agarose, which was possible with an N‐terminal but not with a C‐terminal histidine tag. Purified scFv fragments retained the antigen‐binding properties of the parental antibody, could inhibit its binding to viral antigens with apparently higher efficiency than monovalent antigen‐binding (Fab) fragments, but neutralized viral infectivity with lower efficiency (about sevenfold at a molar level). To evaluate the usefulness of these smaller and less immunogenic molecules in the treatment of viral diseases, mice were treated with purified recombinant scFv fragments and challenged with a lethal viral dose. A small delay in mortality was observed for the scFv‐treated animals. Therefore, even though the scFv could neutralize viral infectivity in vitro, the same quantity of fragments that partially protected mice in the form of Fab only slightly delayed virus‐induced lethality when injected as scFv fragments, probably because of a much faster in vivo clearance: the biologic half‐life was estimated to be about 6 min. Since a scFv derived from a highly neutralizing and protective mAb is only marginally effective in the passive protection of mice from lethal viral infection, the use of such reagents for viral immunotherapy will require strategies to overcome stability limitations.
has issue date	2005-12-06(xsd:dateTime)
bibo:doi	10.1002/eji.1830271245
bibo:pmid	9464834
has license	no-cc
sha1sum (hex)	9b4d9173559b08225b6e1e642e54fb2ae50fce17
schema:url	https://doi.org/10.1002/eji.1830271245
resource representing a document's title	A recombinant single chain antibody neutralizes coronavirus infectivity but only slightly delays lethal infection of mice
has PubMed Central identifier	PMC7163705
has PubMed identifier	9464834
schema:publication	Eur J Immunol
resource representing a document's body	covid:9b4d9173559b08225b6e1e642e54fb2ae50fce17#body_text
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