About: Evaluation of a specialized filter-paper matrix for transportation of extended bovine semen to screen for bovine herpesvirus-1 by real-time PCR

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About: Evaluation of a specialized filter-paper matrix for transportation of extended bovine semen to screen for bovine herpesvirus-1 by real-time PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Evaluation of a specialized filter-paper matrix for transportation of extended bovine semen to screen for bovine herpesvirus-1 by real-time PCR
Creator	Kumar Rana, Samir Kumar Sharma, Girish Laxmi, Narayan Muthappa, Nadikerianda Putla, Bajibabu Reddy, Chandrasekhar Sarangi, Sri, Kota Srinivasan, Villuppanoor Surendra, Naga Thodangala, Naveena Venkata, Rachamreddy
source	Elsevier; Medline; PMC
abstract	The extended frozen semen (EFS) batches produced from infectious bovine rhinotracheitis (IBR) sero-positive cattle and buffalo bulls housed in various semen stations in India are transported to the testing laboratory in liquid nitrogen (LN(2)) for screening bovine herpesvirus-1 (BoHV-1). This procedure is laborious and poses LN(2) related hazards. An alternative logistics for transportation of samples was investigated. Use of Flinders Technology Associates (FTA(®)) elute card was evaluated for transportation of extended bovine semen to screen BoHV-1 DNA by real-time PCR targeting gB gene and the method was compared with the OIE approved Chelex resin based method. A protocol for extraction of BoHV-1 DNA from FTA(®) card spotted with extended semen was optimized. The viral DNA was found to be stable on FTA(®) card for at least 28 days when the cards are stored at 4°–37 °C. The analytical sensitivity for the assay was determined using variable dilutions of BoHV-1 spiked semen and positive plasmid harbouring gB gene (97bp) spotted onto FTA(®) card and it was found to be 10(0.8) TCID(50)/ml or 100 copies respectively in real-time PCR. The test could detect as low as 10(0.008) TCID(50)/ml or 1 copy of positive plasmid when more number of replicates (n = 6) of the same sample were tested. This sensitivity was found to be comparable to Chelex method and both the methods demonstrated a very strong correlation (r = 0.9774; 95% CI: 0.9620–0.9860) in terms of Ct value (p < 0.0001). The diagnostic sensitivity and specificity of the FTA method in comparison to the Chelex method was 83.08% (95% CI: 71.73%–91.24%) and 93.23% (95% CI: 89.38%–96.01%) respectively when 316 samples were screened by both the methods. The degree of agreement between these two tests was good (Kappa value: 0.738; 95% CI: 0.646–0.829). The method was found to be robust and highly repeatable in inter-assay and intra-assay precision testing. The result suggests that the FTA(®) card holds promise as an alternative system for transportation of EFS for downstream screening of BoHV-1 DNA.
has issue date	2018-03-26(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2018.03.009
bibo:pmid	29588253
has license	no-cc
sha1sum (hex)	9b888ba75d7dbaaabcf5beaa505595e7f63f0341
schema:url	https://doi.org/10.1016/j.jviromet.2018.03.009
resource representing a document's title	Evaluation of a specialized filter-paper matrix for transportation of extended bovine semen to screen for bovine herpesvirus-1 by real-time PCR
has PubMed Central identifier	PMC7119822
has PubMed identifier	29588253
schema:publication	J Virol Methods
resource representing a document's body	covid:9b888ba75d7dbaaabcf5beaa505595e7f63f0341#body_text
is schema:about of	named entity 'EVALUATION' named entity 'EFS' named entity 'harbouring' named entity 'based' named entity 'FTA' named entity 'DNA' named entity 'FTA' named entity 'transportation' named entity 'optimized' named entity 'method' named entity 'logistics' named entity '95% CI' named entity 'MATRIX' named entity 'REAL-TIME PCR' named entity 'BOVINE HERPESVIRUS-1' named entity 'TRANSPORTATION' named entity 'SCREEN' named entity 'STABLE' named entity 'INDIA' named entity 'PLASMID' named entity 'METHODS' named entity 'RESULT' named entity 'LABORATORY' named entity 'INTRA' named entity 'EVALUATED' named entity '100' named entity 'PROMISE' named entity 'TRANSPORTATION' named entity '008' named entity '316' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'TCID' named entity 'SCREENING' named entity 'REAL-TIME PCR ' named entity 'TESTING' named entity '646' named entity 'AGREEMENT' named entity 'TARGETING' named entity 'CATTLE' named entity 'APPROVED' named entity 'BUFFALO' named entity 'ALTERNATIVE' covid:arg/9b888ba75d7dbaaabcf5beaa505595e7f63f0341 named entity 'FROZEN SEMEN' named entity 'RESIN' named entity 'VALUE' named entity 'METHOD' named entity 'CT VALUE' named entity 'LOW' named entity 'DOWNSTREAM' named entity 'TEST' named entity '0.8' named entity 'EXTRACTION' named entity 'SYSTEM' named entity 'SAMPLE' named entity 'PAPER' named entity 'INVESTIGATED' named entity 'PROTOCOL' named entity 'BOVINE' named entity 'EXTENDED' named entity 'VERY STRONG' named entity 'VIRAL DNA' named entity 'USE OF' named entity 'VARIOUS' named entity 'POSITIVE' named entity 'GOOD' named entity 'COPY' named entity 'TESTS' named entity 'USING' named entity 'DNA'

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