About: Detection of negative-stranded subgenomic RNAs but not of free leader in LDV-infected macrophages

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Detection of negative-stranded subgenomic RNAs but not of free leader in LDV-infected macrophages Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Detection of negative-stranded subgenomic RNAs but not of free leader in LDV-infected macrophages
Creator	Chen, Zongyu Faaberg, Kay Plagemann, Peter
source	Elsevier; Medline; PMC
abstract	Abstract The mechanism of synthesis of the seven subgenomic mRNAs of lactate dehydrogenase-elevating virus (LDV) was explored. One proposed mechanism, leader-primed transcription, predicts the formation of free 5'-leader in infected cells which then primes reinitiation of transcription at specific complementary sites on the antigenomic template. No free LDV 5'-leader of 156 nucleotides was detected in LDV-infected macrophages. Another mechanism, independent replication of the subgenomic mRNAs, predicts the presence of negative complements to all subgenomic mRNAs in infected cells which might be generated from subgenomic mRNAs in virions. Full-length antigenomic RNA was detected in LDV-infected macrophages by Northern hybridization at a level of < 1% of that of genomic RNA, but no negative polarity subgenomic RNAs. Negative complements to all subgenomic mRNAs, however, were detected by reverse transcription of total RNA from infected macrophages using as primer an oligonucleotide complementary to the antileader followed by polymerase chain reaction amplification using this sense primer in combination with various oligonucleotide primers complementary to a segment downstream of the junction between the 5' leader and the body of each subgenomic RNA. It is unclear whether these minute amounts of negative subgenomic RNAs function in the replication of the subgenomic mRNAs. They could also be by-products of the RNA replication process. Finally, no subgenomic mRNAs were detected in LDV virions.
has issue date	1994-11-30(xsd:dateTime)
bibo:doi	10.1016/0168-1702(94)90098-1
bibo:pmid	7856308
has license	els-covid
sha1sum (hex)	9be2ef59dd6982102f648bde97e9e3f09f20d4a0
schema:url	https://doi.org/10.1016/0168-1702%2894%2990098-1
resource representing a document's title	Detection of negative-stranded subgenomic RNAs but not of free leader in LDV-infected macrophages
has PubMed Central identifier	PMC7133802
has PubMed identifier	7856308
schema:publication	Virus Research
resource representing a document's body	covid:9be2ef59dd6982102f648bde97e9e3f09f20d4a0#body_text
is schema:about of	covid:arg/9be2ef59dd6982102f648bde97e9e3f09f20d4a0 named entity 'lactate' named entity 'mRNAs' named entity 'transcription' named entity 'oligonucleotide primers' named entity 'transcription' named entity 'subgenomic' named entity 'macrophages' named entity 'ions' named entity 'mRNAs' named entity 'primer' named entity 'mRNAs' named entity 'mRNAs' named entity 'macrophages' named entity 'mRNAs' named entity 'denaturation' named entity 'replicase' named entity 'riboprobe' named entity 'nucleotides' named entity 'RNA' named entity 'antisense' named entity '5'-end' named entity 'mRNAs' named entity 'transcription' named entity 'mRNAs' named entity 'PCR' named entity 'genome' named entity 'replicase' named entity 'oligonucleotides' named entity 'coding segments' named entity 'mouse hepatitis virus' named entity 'subgenomic' named entity 'PCR' named entity 'ORFs' named entity '5'-end' named entity 'RNA' named entity 'mRNA' named entity 'ORF' named entity 'primers' named entity 'nucleocapsid' named entity 'coronaviruses' named entity 'ORF' named entity 'genome' named entity 'antisense RNA' named entity 'Southern hybridization' named entity 'Cetus' named entity 'ORF' named entity 'RNA viruses' named entity 'subgenomic mRNA' named entity 'subgenomic' named entity 'mRNAs' named entity 'ultraviolet light' named entity 'antisense RNA' named entity 'hybridize' named entity 'subgenomic' named entity 'oligonucleotide' named entity 'cDNA' named entity 'PCR' named entity 'nucleocapsid' named entity 'subgenomic' named entity 'RNA' named entity 'primer extension' named entity 'PCR' named entity 'non-specific' named entity 'secondary structure' named entity 'Sindbis' named entity 'oligonucleotide' named entity 'mRNA' named entity 'plasma' named entity 'PCR' named entity 'subgenomic' named entity 'coronavirus' named entity 'simian hemorrhagic fever virus' named entity 'RNA' named entity 'Base pairing' named entity 'translation initiation codon' named entity 'electrophoresed' named entity 'mRNAs' named entity 'subgenomic'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software