About: Field evaluation of TaqMan Array Card (TAC) for the simultaneous detection of multiple respiratory viruses in children with acute respiratory infection

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About: Field evaluation of TaqMan Array Card (TAC) for the simultaneous detection of multiple respiratory viruses in children with acute respiratory infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Field evaluation of TaqMan Array Card (TAC) for the simultaneous detection of multiple respiratory viruses in children with acute respiratory infection
Creator	Whitaker, Brett Erdman, Dean Iwane, Marika Prill, Mila Hall, Caroline Schnabel, Kenneth Shelley, Lynne Szilagyi, Peter Weinberg, Geoffrey
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: Multipathogen reverse transcription real-time PCR (RT-qPCR) platforms have proven useful in surveillance for acute respiratory illness (ARI) and study of respiratory outbreaks of unknown etiology. The TaqMan(®) Array Card (TAC, Life Technologies™), can simultaneously test 7 clinical specimens for up to 21 individual pathogens (depending on arrangement of controls and use of duplicate wells) by arrayed singleplex RT-qPCR on a single assay card, using minimal amounts of clinical specimens. A previous study described the development of TAC for the detection of respiratory viral and bacterial pathogens; the assay was evaluated against well-characterized analytical materials and a limited collection of human clinical specimens. OBJECTIVES: We wished to compare TAC assay performance against standard individual RT-qPCR assays for respiratory viral detection, focusing on 10 viruses (adenovirus, human metapneumovirus, human parainfluenza viruses 1–4, influenza viruses A and B, respiratory syncytial virus, and rhinovirus) from a larger collection of human specimens. STUDY DESIGN: We used specimens from 942 children with ARI enrolled systematically in a population-based, ARI surveillance study (New Vaccine Surveillance Network, NVSN). RESULTS: Compared with standard individual RT-qPCR assays, the sensitivity of TAC for the targeted viruses ranged from 54% to 95% (54%, 56%, and 75% for adenovirus, human parainfluenza viruses-1 and -2, respectively, and 82%–95% for the other viruses). Assay specificity was 99%, and coefficients of variation for virus controls ranged from 1.5% to 4.5%. CONCLUSION: The TAC assay should prove useful for multipathogen studies and rapid outbreak response.
has issue date	2013-04-19(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2013.03.016
bibo:pmid	23608639
has license	no-cc
sha1sum (hex)	9d380a825926b91293024e97a338002bce2e0a22
schema:url	https://doi.org/10.1016/j.jcv.2013.03.016
resource representing a document's title	Field evaluation of TaqMan Array Card (TAC) for the simultaneous detection of multiple respiratory viruses in children with acute respiratory infection
has PubMed Central identifier	PMC7108303
has PubMed identifier	23608639
schema:publication	J Clin Virol
resource representing a document's body	covid:9d380a825926b91293024e97a338002bce2e0a22#body_text
is schema:about of	named entity 'Study design' named entity 'development' named entity 'Surveillance' named entity 'single' named entity 'adenovirus' named entity 'detection' named entity 'ARI' named entity 'respiratory' named entity 'wished' named entity 'study' named entity 'Array' named entity 'multiple' named entity 'FIELD' named entity 'ARRAY' named entity 'TECHNOLOGIES' named entity 'USED' named entity 'HUMAN METAPNEUMOVIRUS' named entity 'QPCR' named entity 'TO COMPARE' named entity 'RT-QPCR' named entity 'VARIATION' named entity 'COLLECTION' named entity 'ACUTE RESPIRATORY ILLNESS' named entity 'REVERSE TRANSCRIPTION' named entity 'ANALYTICAL' named entity 'ADENOVIRUS' named entity 'LIFE' named entity 'STUDY' named entity 'SPECIFICITY' named entity 'BACKGROUND' named entity 'TAC' named entity 'CARD' named entity 'EVALUATED' named entity 'RESPIRATORY' named entity 'LIMITED' named entity 'TEST' named entity 'COEFFICIENTS' named entity 'USE OF' named entity 'ARI' named entity 'WELLS' named entity 'HUMAN PARAINFLUENZA VIRUSES' named entity 'VIRAL AND' named entity 'LARGER' named entity 'VIRUSES' named entity 'RESPIRATORY VIRUSES' named entity 'CHARACTERIZED' named entity 'COMPARED' named entity 'CHILDREN' named entity 'REAL-TIME PCR ' named entity 'RHINOVIRUS' named entity 'VIRUS' named entity 'PERFORMANCE' named entity 'TAQMAN' named entity 'SIMULTANEOUSLY' named entity 'MINIMAL' named entity 'TARGETED' named entity 'UP TO' named entity 'BACTERIAL PATHOGENS' named entity 'STANDARD' named entity 'SENSITIVITY' named entity 'DESCRIBED' named entity 'CLINICAL' named entity 'SURVEILLANCE' named entity 'VACCINE' named entity 'ASSAY' named entity 'DEVELOPMENT' named entity 'TAQMAN' named entity 'SIMULTANEOUS' named entity 'MULTIPLE' named entity 'INDIVIDUAL' named entity 'DETECTION' named entity 'STUDY DESIGN' named entity 'HUMAN'

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