About: SARS-Cov-2RNA Found on Particulate Matter of Bergamo in Northern Italy: First Evidence

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About: SARS-Cov-2RNA Found on Particulate Matter of Bergamo in Northern Italy: First Evidence Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS-Cov-2RNA Found on Particulate Matter of Bergamo in Northern Italy: First Evidence
Creator	Pallavicini, Alberto Barbieri, Pierluigi Borelli, Massimo Clemente, Libera De Gennaro, Gianluigi Fontana, Francesco Miani, Alessandro Palmisani, Jolanda Passarini, Fabrizio Perrone, Maria Piscitelli, Prisco Ruscio, Maurizio Setti, Leonardo Torboli, Valentina Gilio, Alessia Barbieri, G Borelli, M Clemente, F Fontana, V Gennaro, De Gilio, Di Ruscio, A Sars-Cov, A Setti, L Torboli, A
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: The burden of COVID-19 was extremely severe in Northern Italy, an area characterized by high concentrations of particulate matter (PM), which is known to negatively affect human health. Consistently with evidence already available for other viruses, we initially hypothesized the possibility of SARS-CoV-2 presence on PM, and we performed a first experiment specifically aimed at confirming or excluding this research hyphotesys. METHODS: We have colelcted 34 PM10 samples in Bergamo area (the epicenter of the Italian COVID-19 epidemic) by using two air samplers over a continuous 3-weeks period. Filters were properly stored and underwent RNA extraction and amplification according to WHO protocols in two parallel blind analyses performed by two different authorized laboratories. Up to three highly specific molecular marker genes (E, N, and RdRP) were used to test the presence of SARS-CoV-2 RNA on particulate matter. RESULTS: The first test showed positive results for gene E in 15 out of 16 samples, simultaneously displaying positivity also for RdRP gene in 4 samples. The second blind test got 5 additional positive results for at least one ofthe three marker genes. Overall, we tested 34 RNA extractions for the E, N and RdRP genes, reporting 20 positive results for at least one of the three marker genes, with positivity separately confirmed for all the three markers. Control tests to exclude false positivities were successfully accomplished. CONCLUSION: This is the first evidence that SARS-CoV-2 RNA can be present on PM, thus suggesting a possible use as indicator of epidemic recurrence.
has issue date	2020-05-30(xsd:dateTime)
bibo:doi	10.1016/j.envres.2020.109754
bibo:pmid	32526492
has license	no-cc
sha1sum (hex)	9d3fdd7495d14cde6f299157f16e167517015844
schema:url	https://doi.org/10.1016/j.envres.2020.109754
resource representing a document's title	SARS-Cov-2RNA Found on Particulate Matter of Bergamo in Northern Italy: First Evidence
has PubMed Central identifier	PMC7260575
has PubMed identifier	32526492
schema:publication	Environ Res
resource representing a document's body	covid:9d3fdd7495d14cde6f299157f16e167517015844#body_text
is schema:about of	named entity 'RdRP' named entity 'RdRP' named entity 'hypothesized' named entity 'possibility' named entity 'viruses' named entity 'SARS-CoV-2' named entity 'indicator' named entity 'molecular marker' named entity 'protocols' named entity 'specific' named entity 'exclude' named entity 'blind test' named entity 'PM10' named entity 'samples' named entity 'genes' named entity 'Italian' named entity 'severe' named entity 'Bergamo' named entity 'extractions' named entity 'epidemic' named entity 'area' named entity 'SARS-CoV-2' named entity 'RdRP' named entity 'Bergamo' named entity 'Italian' named entity 'particulate matter' named entity 'molecular marker' named entity 'gene' named entity 'COVID' named entity 'epidemic' named entity 'WHO' named entity 'PM10' named entity 'Piedmont' named entity 'viruses' named entity 'dehydration' named entity 'RNA' named entity 'Lombardy' named entity 'PM10' named entity 'lysis buffer' named entity 'SARS-CoV-2' named entity 'environmental pollution' named entity 'particulate matter' named entity 'marker gene' named entity 'RNA' named entity 'diagnostic tests' named entity 'gene' named entity 'submicrometre' named entity 'aerosol' named entity 'DNA polymerases' named entity 'blind test' named entity 'lysis buffer' named entity 'Italy' named entity 'RNA' named entity 'Italian' named entity 'urban area' named entity 'SARS-CoV-2 virus' named entity 'Particulate matter'

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