About: Restricted growth of U‐type infectious haematopoietic necrosis virus (IHNV) in rainbow trout cells may be linked to casein kinase II activity

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About: Restricted growth of U‐type infectious haematopoietic necrosis virus (IHNV) in rainbow trout cells may be linked to casein kinase II activity Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Restricted growth of U‐type infectious haematopoietic necrosis virus (IHNV) in rainbow trout cells may be linked to casein kinase II activity
Creator	Park, J Bremont, M Harmache, A Kurath, G Moon, C Purcell, M Wargo, A
source	Medline; PMC
abstract	Previously, we demonstrated that a representative M genogroup type strain of infectious haematopoietic necrosis virus (IHNV) from rainbow trout grows well in rainbow trout‐derived RTG‐2 cells, but a U genogroup type strain from sockeye salmon has restricted growth, associated with reduced genome replication and mRNA transcription. Here, we analysed further the mechanisms for this growth restriction of U‐type IHNV in RTG‐2 cells, using strategies that assessed differences in viral genes, host immune regulation and phosphorylation. To determine whether the viral glycoprotein (G) or non‐virion (NV) protein was responsible for the growth restriction, four recombinant IHNV viruses were generated in which the G gene of an infectious IHNV clone was replaced by the G gene of U‐ or M‐type IHNV and the NV gene was replaced by NV of U‐ or M‐type IHNV. There was no significant difference in the growth of these recombinants in RTG‐2 cells, indicating that G and NV proteins are not major factors responsible for the differential growth of the U‐ and M‐type strains. Poly I:C pretreatment of RTG‐2 cells suppressed the growth of both U‐ and M‐type IHNV, although the M virus continued to replicate at a reduced level. Both viruses induced type 1 interferon (IFN1) and the IFN1 stimulated gene Mx1, but the expression levels in M‐infected cells were significantly higher than in U‐infected cells and an inhibitor of the IFN1‐inducible protein kinase PKR, 2‐aminopurine (2‐AP), did not affect the growth of U‐ or M‐type IHNV in RTG‐2 cells. These data did not indicate a role for the IFN1 system in the restricted growth of U‐type IHNV in RTG‐2 cells. Prediction of kinase‐specific phosphorylation sites in the viral phosphoprotein (P) using the NetPhosK program revealed differences between U‐ and M‐type P genes at five phosphorylation sites. Pretreatment of RTG‐2 cells with a PKC inhibitor or a p38MAPK inhibitor did not affect the growth of the U‐ and M‐type viruses. However, 100 μm of the casein kinase II (CKII) inhibitor, 5,6‐dichloro‐1‐β‐d‐ribofuranosylbenzimidazole (DRB), reduced the titre of the U type 8.3‐fold at 24 h post‐infection. In contrast, 100 μm of the CKII inhibitor reduced the titre of the M type only 1.3‐fold at 48 h post‐infection. Our data suggest that the different growth of U‐ and M‐type IHNV in RTG‐2 cells may be linked to a differential requirement for cellular protein kinases such as CKII for their growth.
has issue date	2011-01-17(xsd:dateTime)
bibo:doi	10.1111/j.1365-2761.2010.01225.x
bibo:pmid	21241319
has license	no-cc
sha1sum (hex)	9d9c8b8fa31178c514cf5d470ed20ca9a6ebf157
schema:url	https://doi.org/10.1111/j.1365-2761.2010.01225.x
resource representing a document's title	Restricted growth of U‐type infectious haematopoietic necrosis virus (IHNV) in rainbow trout cells may be linked to casein kinase II activity
has PubMed Central identifier	PMC7194290
has PubMed identifier	21241319
schema:publication	J Fish Dis
resource representing a document's body	covid:9d9c8b8fa31178c514cf5d470ed20ca9a6ebf157#body_text
is schema:about of	named entity 'data' named entity 'CKII' named entity 'IHNV' named entity 'inhibitor' named entity 'protein kinases' named entity '3-fold' named entity 'M-type' named entity 'serotypes' named entity 'restricted growth' named entity 'U-type' named entity 'M-type' named entity 'M-type' named entity 'antiviral' named entity 'vesicular stomatitis virus' named entity 'phosphorylation sites' named entity 'RNA synthesis' named entity 'poly I:C' named entity 'kinase' named entity 'rhabdovirus' named entity 'Phylogenetic analysis' named entity 'recombinant' named entity 'M-type' named entity 'rabies virus' named entity 'recombinant' named entity 'host-specific' named entity 'virus infection' named entity 'phosphorylation' named entity 'protein kinase' named entity 'salmonid fish' named entity 'phosphorylation sites' named entity 'swine' named entity 'RNA synthesis' named entity 'recombinant' named entity 'necrosis' named entity 'spike protein' named entity 'antiviral' named entity '2.2' named entity 'VSV' named entity 'plaque assay' named entity 'M-type' named entity 'rainbow trout' named entity 'Assay' named entity 'glycoprotein' named entity 'Poly I:C' named entity 'incubation' named entity 'PKR' named entity 'bisindolylmaleimide' named entity 'nucleotides' named entity 'nucleocapsid' named entity 'Mx1' named entity 'ISGs' named entity 'viral proteins' named entity 'transfected' named entity 'luciferase' named entity 'FBS' named entity 'protein' named entity 'poly I:C' named entity 'phosphorylation' named entity 'VSV' named entity 'rainbow trout' named entity 'supernatants' named entity 'phosphoprotein' named entity 'vaccinia virus' named entity 'trout' named entity 'protein' named entity 'M type' named entity 'supernatants' named entity 'infection' named entity 'Qanungo' named entity 'M-type' named entity '3.5' named entity 'virus particle' named entity 'haematopoietic' named entity 'M-type' named entity 'protein kinase'

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